FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367236
Sequences flagged as poor quality0
Sequence length15
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGTAGTCATCAT286597.803973466653597No Hit
CTGATGGGGATTTTG161974.410515308956638No Hit
NNNNNNNNNNNNNNN159504.343256107788997No Hit
GCATCCACCCTTGGG144763.9418793364484963No Hit
CTAAGCGATGAGCGG141363.849295820671176No Hit
CTATTCGTCGGTTGT138083.7599799583918783No Hit
GTTAGGCTCTATGGG135743.6962607151804288No Hit
GTAAGTCTGGAAGAC108252.947695759674977No Hit
ACAGCTCGGTTGTTG94922.584713916936248No Hit
GTTCATGATACGAAT91862.5013887527366596No Hit
TGCCTAAGAGGGGAT87842.391922360552887No Hit
CCTGACTGAAAAACG86472.3546166497837904No Hit
ACACTGGCTACATCG82522.2470563888071977No Hit
GGACTTCTTCCTCGT66841.8200829983988496No Hit
AGCTAGTTTCGCTCG65631.7871341589604506No Hit
TGTGACTATTTATTC63191.720691871167315No Hit
CTGATGGGAGGATCT60271.6411789693820868No Hit
CAGTCAACTCTATAT29690.8084719363025411No Hit
CCTGACTGGGGGCAC25320.6894748880828676No Hit
ACCGATCACCCTTGC22160.6034266793015935No Hit
ACAATCGACGGCGTC21220.5778300602337462No Hit
CGATGATGGAAGGTA13400.36488797394590944No Hit
GTGATGGGGATTTTG11610.316145475933732No Hit
TAGTGGCAATGCCCC11050.300896426276291No Hit
TCCGCATACCCTCCT10240.27883976516463527No Hit
GCTTGTAGGCATCAT9860.2684921957542289No Hit
TGCACGTCGTTAGTC8340.22710191811260333No Hit
ATTCAGCTTTTCTGG8040.21893278436754568No Hit
AGATGCGGGCAGGTG7660.20858521495713928No Hit
GGCCTAAGAGGGGAT7510.20450064808461044No Hit
ACATGCTCGGGGTAT7160.19496999204870982No Hit
CTGATGGGGGTTTTG6620.18026555130760602No Hit
CTGCTGGGGATTTTG6310.17182411310437976No Hit
CTAAGCGGTGAGCGG6300.17155180864621117No Hit
GTAAGCGATGAGCGG6100.16610571948283936No Hit
GCATCCAGCCTTGGG5910.16093193477763618No Hit
CTGATGGGGTTTTTG5790.1576642812796131No Hit
GCTGACTGAAAAACG5370.14622749403653237No Hit
TGATGACATCGGAGC5330.145138276203858No Hit
CTGATGGGGCTTTTG5100.13887527366598046No Hit
GCAGCTCGGTTGTTG4690.1277107908810683No Hit
CATGTGCGGTGTTCT4440.12090317942685357No Hit
GTTAGGCGCTATGGG4350.11845243930333628No Hit
GCATCCACGCTTGGG4260.11600169917981897No Hit
ACAGCTCGGGTGTTG4220.1149124813471446No Hit
GGCTAGTTTCGCTCG4070.11082791447461578No Hit
CTGATGGGGAGTTTG3950.10756026097659271No Hit
GGCGATTGCAACGAT3940.10728795651842411No Hit
CTAAGCGATGGGCGG3920.10674334760208694No Hit
GCATCAACCCTTGGG3860.1051095208530754No Hit
ATGATGGGGATTTTG3690.10048034506420939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)