FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352532
Sequences flagged as poor quality0
Sequence length15
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN5734616.266892083555533No Hit
GGTCTTATTGGAGGA197655.606583232160485No Hit
CTACGATCGTCAGAT162654.613765558871251No Hit
CATGAGGGGACATGG122293.468904950472581No Hit
CGATGATTTCCCATC117313.3276411786731415No Hit
TCGAAGGCTGTTTTG102352.9032825388900863No Hit
AGCTGTTGGGCCTTT101742.885979145155617No Hit
GCATCGTTATCAGAA83542.3697139550452158No Hit
AGCCTTCAAGATTGT57621.6344615524264465No Hit
AGCCGATGGCGCTCA54191.5371654204441014No Hit
TTGCACCTTTGCGTG43771.2415894159962784No Hit
GGTATCAAGTTATAT28020.7948214630161234No Hit
TCTACAGTGCTTCGC26850.7616329865090261No Hit
GCTGAATAGGCGGCG20180.572430304199335No Hit
ATACGATCGTCAGAT15760.4470516151725234No Hit
GGTCTTATTGGAGGG11080.31429770914413446No Hit
AGATGATTTCCCATC8530.2419638500902046No Hit
AATGAGGGGACATGG8320.2360069440504692No Hit
GGTCTTCTTGGAGGA5920.1679280178820646No Hit
AGGCATGTCAGCGGC5370.15232659730180523No Hit
GCTTCATCGGGGCGG5180.14693701564680653No Hit
TAGACGCAGAGGTTC5090.14438405591549136No Hit
TTGCCTAAAGGGGCG4950.1404127852223344No Hit
AGCTGGAGGGATATT4720.13388855479786232No Hit
GACCTAAAAAATAGC4540.128782635335232No Hit
TCGCAGGCTGTTTTG4510.1279316487581269No Hit
CTCATGAACAAGGTT4300.12197474271839151No Hit
AACGACTTCTCAGTA4130.11715248544812953No Hit
GGTCTTATTGGAGGT4100.11630149887102448No Hit
CTACGATCGTCAGCT3800.1077916330999739No Hit
TCGAAGGCTGGTTTG3800.1077916330999739No Hit
AGGTCTATGCCGGAA3750.10637332213813214No Hit
AGGACATGTCCTTTT3710.10523867336865873No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)