FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325687
Sequences flagged as poor quality0
Sequence length15
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN4211612.931434168388666No Hit
GTTAGGCCTGTCGTA198296.08836091093596No Hit
GGACTAATCTAGAAG138154.241802712420207No Hit
ACAGCTCGGTGTAAG108463.330191257250059No Hit
GCATCCAACGATTTC107233.292424935597675No Hit
ATTCCGTGCCGAGCC106133.2586501763963565No Hit
AGTTGCAGGTAGGGG99003.039728328118715No Hit
AGTGTACGACGAGGC92432.8380009027072006No Hit
ACAGCTCTGGCTAAG92212.8312459508669363No Hit
CAGCACTGCGATGAG78672.4155093694252456No Hit
GGTCTAGATATTTAT53571.6448307731042382No Hit
CTGTATGTTCATTAT27020.8296309032905828No Hit
ACGCTACCGGACAGT24590.7550193897822143No Hit
AGCACTGGGGCGCTT23390.7181741979262298No Hit
AACAGCTGTCGCGAA19000.5833822043864201No Hit
AGTGTACACAACATT14970.4596437684034057No Hit
ACGTCAAAACAAGTT12930.39700694224823224No Hit
TAGCCGAGGGGACCT10590.3251588181290626No Hit
TCGACTCTATCACGC9510.2919981454586766No Hit
GTTAGGCCTGTCGGA6720.20633307439351278No Hit
GATAGGCCTGTCGTA6510.19988516581871552No Hit
TGGAGCATCAATGCG6150.1888316082619202No Hit
GTTAGGCCTGGCGTA5320.1633470172281976No Hit
AGTTGCAGGGAGGGG5250.16119771436993185No Hit
GGACTACTCTAGAAG5200.1596624980425992No Hit
TCTGGAGTTATACAC4810.14768781068940426No Hit
GTTAGGCCTGTCGTG4780.14676668089300463No Hit
GTTAGGCCTGTCGTT4550.13970468578727427No Hit
GTTAGGCCTGTAGTA4500.1381694694599416No Hit
ATTCCGTGCCGCGCC4270.13110747435421127No Hit
AGCTGTTCAAAGCGT4250.13049338782327818No Hit
TGTGTACGACGAGGC4160.12772999843407934No Hit
GGACTAATCTAGCAG4100.12588773884128013No Hit
GGACTAATCTAGACG3990.12251026292114822No Hit
TGTTGCAGGTAGGGG3970.12189617639021513No Hit
TTTCCGTGCCGAGCC3840.11790461393915017No Hit
ACAGCTCGGGGTAAG3780.11606235434635094No Hit
TAGCACTGCGATGAG3730.11452713801901825No Hit
GGACTAATATAGAAG3640.11176374862981943No Hit
ATTCCGTGACGAGCC3560.10930740250608714No Hit
TCAGCTCGGTGTAAG3550.10900035924062058No Hit
GTTAGGCCTGTCGTC3540.10869331597515405No Hit
GTTCGGCCTGTCGTA3410.10470175352408909No Hit
ACAGATCCACAGTGA3360.10316653719675639No Hit
GGACTCATCTAGAAG3300.10132427760395717No Hit
CTTCCGTGCCGAGCC3300.10132427760395717No Hit
GCATCCAACGATTTT3280.1007101910730241No Hit
CAGCACTGCGATGCG3270.10040314780755757No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)