FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002271284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314377
Sequences flagged as poor quality0
Sequence length15-160
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA5904418.78127216685699No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG5337216.97706893315987No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA275558.764954179217947No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG120513.8332956927510597No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC100753.204750983691555No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA98733.140496919303893No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA73192.3280965210559295No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA70222.233623961040407No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC69452.2091310751104567No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC57581.831558924476027No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG41121.3079837265448808No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA34961.112040639105278No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG33691.071643281792243No Hit
CAGATGGCTATAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACT27700.8811077146228891No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC26640.8473902352907496No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG25610.8146270242415953No Hit
CAGATGGCTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACT23520.7481463338603015No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC22590.7185640170877641No Hit
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC22010.700114830283386No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA20400.6489024324298533No Hit
CCTGAATGCCCCAACAGCTCCATCTTCTGCTCTGTGACTTCTCTCTAGAC17810.5665172706654749No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC16830.535344506754629No Hit
CAGATGGCTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT13250.42146849165174294No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC10720.3409918664533347No Hit
CAGATGGCTATAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT10470.3330396307617923No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGC9100.28946137917214043No Hit
GGGGTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGG8350.2656046720975135No Hit
GGGGGACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG7670.2439745910165184No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT7530.23952133902925468No Hit
CGGTTCTTTGCAGTCAGGCCTGACGAGTGCTGACAGCTATGAAGAAGACC6410.20389532313114508No Hit
GGGGAACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG6410.20389532313114508No Hit
TCTCCACTCTGACGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC6060.19276219316298585No Hit
GGGGCACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG5840.1857642257544286No Hit
GTCTCTAGATTAAACACAGAGGACCTCCCCCTCACTCTGGAGTCTGCTGC5660.18003861605651814No Hit
CGGTTCTTTGCAGTCAGGCCTGACTGGCATTGTATTAGCAAACTCATCAC5480.17431300635860766No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGC5030.15999898211383148No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG5020.1596808926861698No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGG4970.15809044554786134No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGACTCCTTTCCTCTCACTGTGACA4760.15141056756696578No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA4690.14918394157333392No Hit
GGGACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA4580.1456849578690553No Hit
CCCTGATGGCTACAATGTCTCTAGATCAAACACAGAGGACCTCCCCCTCA4380.13932316931582145No Hit
GTGTCTCCAGAGCAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCT4240.13486991732855777No Hit
GGTACAGCGTCTCTCGGGAGACGAAGGAATCCTTTCCTCTCACTGTGACA4080.12978048648597068No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTGTATC3960.12596341335403036No Hit
CAGATGGCTATAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG3950.12564532392636865No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG3780.12023780365611987No Hit
CAGATGGCTACAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG3660.11642073052417956No Hit
TCTCCACTCTGAAGATCCAGCCTGCAAAGCTTGAGGACTCGGCCGTGTAT3510.11164938910925419No Hit
GGGGTACAGTGTCTCTAGAGAGAAGACGGAGCGCTTCTCCCTGATTCTGG3410.10846849483263725No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGGGACA3340.10624186883900541No Hit
CAGACCCCAGGACCCATTAAGCCACCCCCGTGTTCCTGCCGTCAGTGCCA3290.10465142170069694No Hit
TACAAAGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATC3190.10147052742408001No Hit
CCTGAATGCCCCAACAGCTCAGTGCTTTCCTGACACTCCAGTGTCTGGGG3170.10083434856875662No Hit
CAGACCCCAGGACCGGCCGAACGCAGAGGTTGATTCTTCACCACACTGAA3160.10051625914109492No Hit
GGTACAGCGTCTCTCGGGCGAAGAAGGAATCCTTTCCTCTCACTGTGACA3150.10019816971343323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)