FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002271288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253226
Sequences flagged as poor quality0
Sequence length15-160
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC2536510.016743936246673No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA199367.872809269190367No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG147735.8339191078325285No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA112494.442276859406222No Hit
CAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCCTTCTCA90033.55532212332067No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA72662.8693736030265455No Hit
GATAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACAT58442.3078198921121844No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC46991.855654632620663No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC38621.5251198534115769No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA30841.2178844194513991No Hit
CTCAGCAGAGATGCCTGATGCAACTTTAGCCACTCTGAAGATCCAGCCCT23450.9260502476049063No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC20370.8044197673224708No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA18590.7341268274189854No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA17980.710037673856555No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG17530.692266986802303No Hit
CGGTTCTTTGCAGTCAGGCCTGACGAGTGCTGACAGCTATGAAGAAGACC16410.6480377212450538No Hit
CAGACCCCAGGACCGGCCCGTGGTACCCAGCAGGTGGCACTGCAGCTCCC16370.6464581046180092No Hit
CCTGAATGCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCAC15990.6314517466610854No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG15330.6053880723148491No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT15100.5963052767093426No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC14520.5734008356171957No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG13390.5287766659031853No Hit
CGATTCTCAGGGCTGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGC12230.4829677837188915No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG11230.44347736804277604No Hit
CCTGAATGCCCCAACAGCAGCAACCCTATCATCGTCTCCTGTGGCTGGGA10290.40635637730722757No Hit
CGATTCTCAGGGCGCTCTCCCAGCAAAGCAGTGGCCGCCCGGGCGGCGGG10030.3960888692314375No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT9520.3759487572366187No Hit
GTTTCCCATCAGCCGCCCAAACCGTGGCAGCAGGACCGACAAGTCAAGTG8490.3352736290902198No Hit
CTCAGCAGAGATGGAGAGCTAAAGTCCAAGAGAGGATCCGAGAACGCTCT8080.3190825586630125No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA8030.3171080378792067No Hit
GCTGAAAGGACTGGAGGGACTGTCCCTGGATCTGCGGAGGTAGACAGTTT7960.3143437087818786No Hit
CGATTCTCAGGGCGCCCAGTGAAGAAATCTATACGAGATACTCCAGCCAA6730.26577049750025666No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA6550.25866222267855593No Hit
CAGACCCCAGGACCCATTAAGCCACCCCCGTGTTCCTGCCGTCAGTGCCA6020.23773230237021475No Hit
CGGTTCTTTGCAGTCAGGCCTGACTGGCATTGTATTAGCAAACTCATCAC5590.2207514236294851No Hit
CAGATGGCTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT5590.2207514236294851No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC5430.21443295712130667No Hit
GCAGAGAGGCTCAAAGGAGTCAACATTTAGATCTGGAAGGGACAAGTCAT5370.21206353218073973No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA5210.2057450656725613No Hit
CGATTCTCAGGGCGCCCAACGATCTATAAACCTAGCCATGGCCATCCCCT5200.2053501615158001No Hit
CAGATGGCTATAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT4970.19626736591029356No Hit
CAGACCCCAGGACCTCACCAGGTGGTTGTGAATGTCAAAAGGTAAAACCC4920.19429284512648778No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG4700.1856049536777424No Hit
CAGACCCCAGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG4580.18086610379660856No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT4560.18007629548308626No Hit
CGATTCTCAGGGCGCCCACGATGCTCTTAGCTGAGTGTCCCGCGGGGCCC4250.16783426662349046No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG4170.16467503336940126No Hit
GGAACGATTTTCTGCTGAATTTCCCAACCCACCCCCAAACTAAACACTAA4050.15993618348826738No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA4040.15954127933150625No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAT4000.15796166270446163No Hit
CGGTTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC3810.1504584837259997No Hit
CAGACCCCAGTCCACCATGATCCATCTGGGTCACATCCTCTTCCTGCTTT3670.14492982553134354No Hit
GATAACTTCCAATCCAGGAGGCAGTGGAGGAAGTGGGGGTTATGGTGGGA3670.14492982553134354No Hit
GTTTCCCATCAGCCGCCCAAACCTCGCAGGGCTCCAGCAGGCCAACCGGC3470.13703174239612048No Hit
CAGACCCCAGGACCAGCTCAGGACAGATGCCACACAAGGATAGATGCTGG3350.1322928925149866No Hit
TCTCAGCTCAACAGTTCAGTGACTCTAATACCACAGTTCTTTTGCTGTCC3220.12715913847709162No Hit
CAGACCCCAGGACCGACCAAAGCCCGCGCGCCGCTGCATCCCGCGTCCAG3180.125579521850047No Hit
CAGACCCCAGGACCCTGAGAGGCAGAGTGTGGTATAGGGAAAAGGCTCCA3110.12281519275271892No Hit
CGGTTCTTTGCAGTCAGGCCTGAGCCGCCTGGATACCGCAGCTAGGAATA2990.11807634287158505No Hit
GGGGTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG2980.11768143871482391No Hit
CGATTCTCAGGGCGCCATCGCCAAGTAAGAAAGTGAAGCAAATCAGAAAC2950.11649672624454044No Hit
NNNNNNNNNNNNNNN2860.11294258883369007No Hit
CTGCAGAGAGGCCTGAGTGAATTTCACTCCCTGGCTCCAGCCTCATCTGC2630.1038597932281835No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT2540.10030565581733314No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)