FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002271292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222538
Sequences flagged as poor quality0
Sequence length15-160
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA3461315.553748123915915No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG114555.147435494162795No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA98694.434748222775435No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA82883.7243077586749234No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA79623.5778159235726035No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC60602.723130431656616No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA55592.498000341514708No Hit
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC48762.191086466131627No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA44852.015386136300317No Hit
GCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCTGCAGA27341.2285542244470606No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAACCTGCAAA26991.212826573439143No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAGA25051.1256504507095417No Hit
CAGACCCCAGGACCGGCCAGACTGTCCAGGCTACCGTCGGCGACAAGTGC22971.0321832675767735No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG22781.023645399886761No Hit
CTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTGTATC22511.0115126405377957No Hit
TCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCTCC21570.9692726635451023No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG21540.9679245791729952No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG19580.8798497335286558No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC18060.8115467920085557No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCA17340.7791927670779822No Hit
CAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCCTTCTCA16630.7472881036047776No Hit
CCTGAATGCCCCAACAGCTCTTACTTATTCCTTCACCTACACACCCTGCA14660.6587638965030691No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC14040.6309034861461863No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA13870.6232643413709119No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT13690.6151758351382686No Hit
CCTGAATGCCCCAACAGCTCAGGTCGCCCCGGAGTCGGCTCCTGCTGGGG12150.5459741707034305No Hit
CGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCA11750.5279997124086673No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC10740.4826142052143903No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA10720.4817154822996522No Hit
CTGCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGG9430.42374785429904105No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC8730.39229255228320553No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG8500.38195723876371673No Hit
GTTTCCCATCAGCCGCCCAAACCGTGGCAGCAGGACCGACAAGTCAAGTG7130.32039471910415296No Hit
CAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC6500.29208494728990103No Hit
TCTCACCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAG6190.2781547421114596No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG5860.26332581401828004No Hit
CGGTTCTTTGCAGTCAGGCCTGACGAGTGCTGACAGCTATGAAGAAGACC5560.24984497029720767No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT5120.23007306617296824No Hit
TCTCACCTAAATCTCCAGACAACCTTAGCCAAACCATTTACCCAAATAAA4820.21659222245189583No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA4530.2035607401881926No Hit
TCTCCACTCTGACGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC4470.2008645714439781No Hit
CTGCAGAGAGGCCTAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGG4170.18738372772290576No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT4110.1846875589786913No Hit
TCTCAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT3780.16985863088551167No Hit
CCTGAATGCCCCAACAGCTAATCAGTAGAGACCTGGCGAGCATTTTACTT3770.1694092694281426No Hit
GGTACAGCGTCTCTCGGGAGATGAAGGAATCCTTTCCTCTCACTGTGACA3650.16401693193971367No Hit
GTTTCCCATCAGCCGCCCAAATCTAACATTCTCAACTCTGACTGTGAGCA3650.16401693193971367No Hit
CCTGAATGCCCCAACAGCTCTCACTTAATCCTTCACCTACACACCCTGCA3390.1523335340481176No Hit
GATAACTTCCAATCCAGGAGGCCGGCACGGAGGTGGTCAAGGCTAAAGCC3380.15188417259074855No Hit
CGATTCTCAGGGCGCTCTCCCAGCAAAGCAGTGGCCGCCCGGGCGGCGGG3360.15098544967601038No Hit
CGGTTCTTTGCAGTCAGGCCTGTTATAACCGCTGAGCAGCCACACTCGCA3190.14334630490073605No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAACCTGCA2970.13346035283861635No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG2970.13346035283861635No Hit
CAGATGGCTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT2950.13256162992387818No Hit
CAGACCCCAGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG2930.13166290700914002No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC2910.13076418409440185No Hit
GGGGAACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG2880.12941609972229462No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA2590.11638461745859136No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC2490.11189100288490056No Hit
GTTTCCCATCAGCCGCCCAAACCTTAGAAAGCTGGATGATACAGACCCTG2450.11009355705542424No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG2380.10694802685384068No Hit
CGGTTCTTTGCAGTCAGGCCTGACTGGCATTGTATTAGCAAACTCATCAC2330.10470121956699531No Hit
CAGATGGCTATAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT2280.10245441228014991No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)