FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222538
Sequences flagged as poor quality0
Sequence length15
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN199848.980039364063666No Hit
TTCAGTCTGCGTTGG153796.910729852879059No Hit
AACATCGGGTGCTTG16900.7594208629537427No Hit
CAGCACTGTTATTTT14800.6650549569062363No Hit
ATGTCGCTTCGATAT14550.6538209204720093No Hit
AGCCTTCGAAACGAA14070.6322515705182935No Hit
GAGCTGATAGGTTTA10990.4938482416486173No Hit
CAGCACTTTTGAAAT10940.4916014343617719No Hit
TTCGCGAAACAGTTT10430.4686840000359489No Hit
TAGTGGCTTTTGAAT9520.4277921074153627No Hit
GCCGTAACGCACTCG9030.40577339600427786No Hit
AGCTCAAGACCACTG8580.3855521304226694No Hit
AGATCACATCGACTC8520.3828559616784549No Hit
TTCATGGTGTGGGGG8510.38240660022108586No Hit
TGACATGGATAGTTT8280.37207128670159706No Hit
ATCAGGCTGGTTGAA8250.3707232023294898No Hit
GAGCTGAAGCGTGAT8200.3684763950426444No Hit
CATGTGCTGCCGCGG7500.3370210930268089No Hit
TTCAGTCAACCAGGT7440.3343249242825944No Hit
CGACACTTGACATGG7200.32354024930573655No Hit
TATAGGCAACCCGCT7130.32039471910415296No Hit
TGCCTAACGTAGTGC6180.27770538065409056No Hit
GAGTTCTGTGTTGTT5590.2511930546693149No Hit
GACGTCTTCCTGAAT5110.22962370471559915No Hit
ACGATGCGACGCGAA5050.22692753597138465No Hit
CTAGACGCGTGTTTT4960.22288328285506295No Hit
CTACCGATCGGACGG4520.2031113787308235No Hit
GACGTCTTTCTCAAT4450.19996584852923996No Hit
AACATCGGTGAAACG4410.19816840269976363No Hit
CTGATCATCGTTCGC4320.19412414958344193No Hit
CCTGATGTGGATAGC4240.1905292579244893No Hit
TAGTGGCGGGCTATA4010.1801939444050005No Hit
GCGTTAGAATTGTTG3790.17030799234288077No Hit
GTGTCTACCCAATTT3700.16626373922655907No Hit
TATAGGCGGCTACTC3690.16581437776918997No Hit
TGCACAATATCATTG3610.16221948611023737No Hit
CTAGAGTGATCACCG3500.1572765100791775No Hit
GCAATTGCCGACTTT3370.15143481113337945No Hit
CATGAGGGACTAAAG3330.14963736530390315No Hit
CAGCACTGGTTTGGG3190.14334630490073605No Hit
CTGGAAGTAGGGTTG3050.13705524449756895No Hit
AGCCTTCCTTCTGTG3050.13705524449756895No Hit
TTAGGCAAACTCAAG3020.13570716012546172No Hit
ATGACGTCCAAAACG2930.13166290700914002No Hit
CTTCAGTCTGTGGAA2840.1276186538928183No Hit
TAGTGGCTTTAATAC2780.12492248514860384No Hit
GGAGACTGGGGCGGC2530.11368844871437687No Hit
TCGATACCGGGGTTT2520.11323908725700779No Hit
GTTCATGTCTGTTTA2500.11234036434226964No Hit
ACTCGGTTGCCGCCT2450.11009355705542424No Hit
AGCCTTCGGTAATCA2350.10559994248173345No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)