FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002271296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200848
Sequences flagged as poor quality0
Sequence length15-160
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG132306.587070819724369No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG73813.674918346212061No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA67603.3657293077352026No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC64623.2173584003823787No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCC63313.1521349478212377No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA63153.1441687246076633No Hit
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA61013.037620489126105No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA53612.6691826654982873No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA49212.45011152712499No Hit
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC43412.1613359356329163No Hit
CGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCA28931.4403927348044292No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG25951.292021827451605No Hit
TCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT25041.2467139329244006No Hit
CAGACCCCAGGACCGGCCAGACTGTCCAGGCTACCGTCGGCGACAAGTGC22761.1331952521309647No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG21111.0510435752409781No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATC18890.9405122281526328No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC17480.8703098860830081No Hit
CTGCAGAGAGGCCTAAGGGATCCATCTCCACTCTGACGATCCAGCGCACA14780.7358798693539393No Hit
CTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTGTATC13980.6960487532860671No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAACCTGCAAA13370.6656775272843145No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA11390.5670955150163307No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGG9980.496893172946706No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC9790.4874332828805863No Hit
CAGACCCCAGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG9230.4595515016330758No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG8680.43216760933641357No Hit
CAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC8620.42918027563132316No Hit
TCTCCACTCTGAAGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT8300.41324782920417436No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC7670.3818808253007249No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA7570.3769019357922409No Hit
CGGTTCTTTGCAGTCAGGCCTGACGAGTGCTGACAGCTATGAAGAAGACC7350.36594837887357606No Hit
TCTCACCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAG7100.35350115510236596No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT6730.3350792639209751No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG6710.33408348601927823No Hit
CAGACCCCAGGACCGGCTTACACCCACGTATCAAGGTTGACCTCGGATCA6390.31815103959212937No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA6350.31615948378873576No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC6300.31367003903449375No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA6170.30719748267346453No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG6020.2997291484107385No Hit
GATAACTTCCAATCCAGGAGGCCGGCACGGAGGTGGTCAAGGCTAAAGCC5930.2952481478531028No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC5500.27383892296662155No Hit
GTTTCCCATCAGCCGCCCAAACCGTGGCAGCAGGACCGACAAGTCAAGTG5490.27334103401577314No Hit
TCTCAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT5360.2668684776547439No Hit
CAGACCCCAGGACCGGCTGAGAAGCTTTTGCAGCAAGCTGATAAAGCATT5070.2524296980801402No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC4920.24496136381741418No Hit
GGGGTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG4880.24296980801402054No Hit
CTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACA4630.2305225842428105No Hit
CGATTCTCAGGGCGCTCTCCCAGCAAAGCAGTGGCCGCCCGGGCGGCGGG4590.2285310284394169No Hit
GATCCATCTCCACTCTGACGATTCAGCGCACAGAGCAGCGGGACTCAGCC4570.22753525053772006No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG4460.22205847207838764No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT4160.20712180355293555No Hit
TCTCACCTAAATCTCCAGACAACCTTAGCCAAACCATTTACCCAAATAAA4120.20513024774954197No Hit
CAGACCCCAGGACCTCCCTGACCCCCAACCAGGCCAGCGGAAGCCTGCCA4090.20363658089699674No Hit
CAGACCCCAGGACCGGCCCCATCATTGAGGAGGTTGATTGAATGGCCCTT3890.19367880188002867No Hit
CAGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT3860.19218513502748347No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC3800.18919780132239306No Hit
GGGGAACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG3720.1852146897156058No Hit
CAGACCCCAGGACCAGCACCCGCAGGGCCTGCCGATCGTCGAACCCGACG3410.16978013223930535No Hit
CAGATGGCTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT3150.15683501951724688No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCA2890.1438899067951884No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG2860.1423962399426432No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA2790.13891101728670435No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA2590.12895323826973631No Hit
CCTGAATGCCCCAACAGCCCTCCTGTGCCTGCTTCCCTGTGTGTCGGGGT2560.1274595714171911No Hit
CCTGAATGCCCCAACAGCTCTTACTTATTCCTTCACCTACACACCCTGCA2560.1274595714171911No Hit
CAGATGGCTATAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT2440.12148490400701027No Hit
GGGGGACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG2440.12148490400701027No Hit
TCTCCACTCTGAAGATCCAGCGCACAGAGCGGGGGGACTCAGCCGTGTAT2420.12048912610531347No Hit
GCTGAAAGGACTGGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA2360.11750179240022306No Hit
GTTTCCCACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC2350.11700390344937465No Hit
CAGACCCCAGGACCAGCTGACAGCACTCACTGGGCGGATCCAGGAGGCCG2250.11202501394089062No Hit
GTTTCCCATCAGCCGCCCAAACCTCACTAGACATCAGATAATCCATACTG2180.10853979128495181No Hit
GTTTCCCATCAGCCGCCCAAATCTAACATTCTCAACTCTGACTGTGAGCA2140.10654823548155819No Hit
GTTTCCCATCAGCCGCCCAAACCTTAGAAAGCTGGATGATACAGATGAGC2120.10555245757986138No Hit
CAGACCCCAGGACCGGCCCATGTCCCCCGGAGACTGTCCTCCGGAAACAA2080.10356090177646778No Hit
CGGTTCTTTGCAGTCAGGCCTGACTGGCATTGTATTAGCAAACTCATCAC2040.10156934597307415No Hit
TCTCACCTAAATCTCCAGACATAGCTCACTTAAATCTTCACATCAATTCC2020.10057356807137735No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)