FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200848
Sequences flagged as poor quality0
Sequence length15
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN2632313.105930853182507No Hit
CGCTACAGGTGCTGT40352.008981916673305No Hit
TCCTGTAAGCCGGCG36561.8202820043017605No Hit
CTATCGTAGAAGCGT25931.2910260495499084No Hit
TCTAACGGAGCAGAG18660.9290607822831195No Hit
CAATGCGGTCGTAAA15530.7732215406675695No Hit
TTCAGTCGGTGCAGG12670.6308253007249264No Hit
ATGCGCAGCGCACTC11430.5690870708197243No Hit
CGAGTGAGCATGATG11120.5536525133434239No Hit
CAGGAATTGTGAGCT7640.3803871584481797No Hit
TTCAGTCAGGCAGTA7160.35648848880745637No Hit
ATCTGCACGGACGTC5820.2897713693937704No Hit
GCATCCAGCACCGGG5590.27831992352425716No Hit
CTGTATGGTCTTTTG5560.27682625667171196No Hit
CAGCGATTAGATGTT5440.2708515892615311No Hit
AGTGAGCATGTCTTT5400.26886003345813747No Hit
GCTTGTACCGCCTTC5310.26437903290050185No Hit
GTCGAGAATATCCTT5290.2633832549988051No Hit
TCGAAGGGAGACGGG4760.23699514060383972No Hit
ATCGCCAAAGGAGGA4310.21459013781566158No Hit
CATGCTGTTACTAGT4150.20662391460208718No Hit
GTCCATATGCGTAGG4090.20363658089699674No Hit
ACTACTGGGGAGGCT4030.20064924719190633No Hit
GCTAGAACACCATCT3770.18770413446984785No Hit
AGATGCGTTAGTGGT3530.17575479964948618No Hit
ACTACTGCTGCATTT3470.17276746594439576No Hit
ATAGCGAGATTGCTT3370.16778857643591172No Hit
CATGAACAGTAACGC3350.16679279853421491No Hit
CTCGATCCGGGGATG3330.16579702063251814No Hit
GTCCATATATTGTGT3230.1608181311240341No Hit
ATGGTCGGACACTTT3150.15683501951724688No Hit
ACACTGGGAGATGGT3070.15285190791045963No Hit
AGGCTAGGGCGTGGT3070.15285190791045963No Hit
GTTAGGCTGGAGTAA2890.1438899067951884No Hit
ACGCTATGTTTGGTG2860.1423962399426432No Hit
ACGTTGGACCTTTTG2720.13542579463076554No Hit
TACCAGTCGTTGCCG2490.12397434876125228No Hit
CGTTACGGGGTGCGT2470.12297857085955548No Hit
CTACGATTGCTTGGG2440.12148490400701027No Hit
TACCAGTGTGTTTAT2310.11501234764598105No Hit
AATGCCGGGGATCTT2240.1115271249900422No Hit
TACTGACGTTTACAA2170.10804190233410339No Hit
AGATACCATAATCGC2100.10455667967816458No Hit
GTTCGGACTCGCTGG2090.10405879072731616No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)