FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232484
Sequences flagged as poor quality0
Sequence length15
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN4071817.514323566352953No Hit
GTAGTCTCGGTGGGC151076.498081588410385No Hit
ACGTGGTTTGAGGGA39161.6844169921370933No Hit
CTACAAGAGAGTAGT26481.1390031141928045No Hit
TTCCTGATCGGGGAT22340.9609263433182499No Hit
TCGACTCAGCAGCCG21800.9376989384215687No Hit
TACCAGTTAGGATGT19950.8581235697940504No Hit
GAAGTCCCTTTATCT19010.8176906797887167No Hit
CTGTGAGTTGTCAGC17820.7665043615904751No Hit
CCATGGTCACTCAGG16780.7217701003079782No Hit
AAGGTACTTAGTGGC14960.6434851430636087No Hit
GCAATTGCCGACGAT14580.6271399322103887No Hit
CTAAGCGAAGCAATG13680.5884275907159202No Hit
TGGATGCTCGGCTAC12150.5226166101753239No Hit
GCTACTCTGTAGACG11730.5045508508112386No Hit
CGTATGATCTAGTTA11380.4894960513411675No Hit
GAGCTAGGACGAGCT10990.47272070336023125No Hit
GTAGTCTCGGTGGTC10830.4658385093167702No Hit
TTGGTCAATGAGGAG10180.43787959601520965No Hit
TGGATGCGTGACGTG8950.3849727293061028No Hit
GCATACTGGTAGCCA8910.38325218079523754No Hit
TGAGACCGGTATTTG8800.378520672390358No Hit
CACAGATGGGAGTTT8630.37120834121918067No Hit
CCGAAGTGAAAAAAG8540.3673371070697338No Hit
GTAGTCTCGGGGGGC8200.3527124447273791No Hit
ACGTTGGGCCTTGGA7990.3436795650453365No Hit
GACGTCTCTGATTGT7970.3428192907899038No Hit
CTGTGAGGGTGCTAG7920.34066860515132225No Hit
TGCCTAAGGTTGAGC7800.33550695961872645No Hit
CTACGATAGCTTAGT7620.32776449131983276No Hit
CGTAACTCCGAGCAT7390.3178713373823575No Hit
ATCTACGACGCTGCT7240.31141928046661277No Hit
TCACTGAGGTGAAAT6790.29206310971937854No Hit
TCGTGAGGCGCGCTT6250.2688357048226975No Hit
CGTCAGATGGTGTTT6180.26582474492868324No Hit
CTCATGAGCTGACAC6150.26453433354553435No Hit
TAGGTGCGGGAGGTT6100.26238364790695273No Hit
AACGTTCCCTTGGTT6080.2615233736515201No Hit
TTCAGGAACGAGAGT6030.25937268801293856No Hit
AACTCGTTAAAATAG5880.25292063109719376No Hit
TGACGGAGTGCTACA5550.23872610588255538No Hit
CCTGATGGGTCGGGT5240.22539185492334957No Hit
TAGACCAGTCGTCGT4840.20818636981469693No Hit
TAGCGTGGTTAGCGG4520.1944219817277748No Hit
CGTAACTATGCCTAT4490.19313157034462586No Hit
GAGACTACACGATCG4400.189260336195179No Hit
CTAGCTCGAAGGCTG4300.18495896491801586No Hit
CGGTATTAGGCGTAC3490.15011785757299426No Hit
TCCTGTAGGACCGGG3490.15011785757299426No Hit
TCACTGAGTGTCCCC3410.14667676055126372No Hit
ACACGTGTGAAACTT3380.1453863491681148No Hit
TCGAAGGGGCTGTTG3380.1453863491681148No Hit
AGGATCCGAGGTGAT3350.14409593778496585No Hit
GTCACTAGATGTAGC2980.12818086405946216No Hit
ATCTACGAGGCGAGT2910.12516990416544793No Hit
GAAGACCCTTTATCT2730.11742743586655424No Hit
CGACGTTTCGAGGGT2660.11441647597254005No Hit
GCAGTAGTGGAAGGG2520.10839455618451162No Hit
GACCAGTTAGGATGT2490.10710414480136267No Hit
CGTTACGTAAGGTTT2480.10667400767364636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)