FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002271300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283102
Sequences flagged as poor quality0
Sequence length15-160
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA4342415.338641196459227No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG128474.537940388976412No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG98303.47224675205403No Hit
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC80522.8442045623132297No Hit
CAGACCCCAGGACCGGCCAGACTGTCCAGGCTACCGTCGGCGACAAGTGC70252.481437785674421No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG68372.4150306250044156No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA66332.3429717910858985No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG55791.9706678158402273No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA50141.7710931042521776No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA42941.5167678080691764No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCC33191.172368969488029No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAGA32901.1621253117251027No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC28361.001759083298599No Hit
GTTTCCCATCAGCCGCCCAAACCTTAGAAAGCTGGATGATACAGACCCTG27150.9590183043567336No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC23320.8237313759704983No Hit
CAGACCCCAGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG23310.8233781463924663No Hit
TCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCTCC23110.8163135548318274No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGG22410.7915874843695911No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC16460.5814158854405832No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC14620.5164216430827052No Hit
TCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT13340.47120825709461606No Hit
CGGTTCTTTGCAGTCAGGCCTGTTATAACCGCTGAGCAGCCACACTCGCA12970.45813876270743403No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG12850.4539000077710507No Hit
CAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC11120.392791290771524No Hit
CGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCA11100.3920848316154602No Hit
CCTGAATGCCCCAACAGCCCTCCTGTGCCTGCTTCCCTGTGTGTCGGGGT10590.37407012313583093No Hit
CGGTTCTTTGCAGTCAGGCCTGACGAGTGCTGACAGCTATGAAGAAGACC9890.3493440526735947No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA9820.34687144562737104No Hit
GGGGTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG9230.3260309005234862No Hit
CTGCAGAGAGGCCTAAGGGATCCATCTCCACTCTGACGATCCAGCGCACA8980.3172001610726876No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG8480.2995386821710903No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA8370.29565315681273885No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT8310.29353377934454716No Hit
TCTCACCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAG8290.2928273201884833No Hit
GATAACTTCCAATCCAGGAGGCCGGCACGGAGGTGGTCAAGGCTAAAGCC7700.27198677508459845No Hit
CCTGAATGCCCCAACAGCTCTTCCTTAAACCTTCACCTACACGCCCTGCA7550.2666883314141193No Hit
CAGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT7510.26527541310199154No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA6700.23666381728140387No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA6330.22359432289422188No Hit
TCTCAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT5990.21158451724113572No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT5840.20628607357065654No Hit
GTTTCCCATCAGCCGCCCAAACCCACCTTCAGGTCCTACCTCCTGCCACC5820.2055796144145926No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG5790.20451992568049676No Hit
TCTCACCTAAATCTCCAGACAACCTTAGCCAAACCATTTACCCAAATAAA5530.19533595665166617No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG5480.19356980876150645No Hit
CAGACCCCAGGACCGGCTTACACCCACGTATCAAGGTTGACCTCGGATCA5400.19074397213725086No Hit
CCTGAATGCCCCAACAGCTCTTACTTAAACCTTCACCTACACGCCCTGCA5250.1854455284667717No Hit
CGATTCTCAGGGCGCTCTCCCAGCAAAGCAGTGGCCGCCCGGGCGGCGGG5150.18191323268645224No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC5090.17979385521826055No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC5030.1776744777500689No Hit
GCTGAAAGGACTGGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA5020.17732124817203693No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACTCCCTGCA4690.1656646720969827No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT4440.15683393264618406No Hit
CAGACCCCAGGACCGGCCACAGCAGAGAAAGTGTCGGTAGCCGTGCTGGC4250.1501225706635771No Hit
GTTTCCCATCAGCCGCCCAAACCTTTGCCTGTCAGATCGGAGCACGCCCC4120.14553058614916178No Hit
CTCCGCACAACAGTTCCCTGACTTAAGACTCATCCAGTATGATTGCGGAA4120.14553058614916178No Hit
TCTCCACTCTGAAGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT3880.13705307627639507No Hit
CGATTCTCAGGGCGCCAGAGAGCTCTGGTGTTGCCCAGCAAATGAACGAT3860.1363466171203312No Hit
CGGTTCTTTGCAGTCAGGCCTGACTGGCATTGTATTAGCAAACTCATCAC3850.13599338754229923No Hit
TCTCCACTCTGACGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC3840.1356401579642673No Hit
CAGACCCCAGGACCAGCGAGCGCAGGGGGCAGTGCTGAGGCCCTGCTGAG3770.1331675509180437No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACACCCTGCA3730.13175463260591588No Hit
CAGACCCCAGGACCGGCCCATGTCCCCCGGAGACTGTCCTCCGGAAACAA3650.12892879598166032No Hit
GGTACAGCGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAG3550.12539650020134085No Hit
GCAGAGAGGCTCAAAGGAGTCAACATTTAGATCTGGAAGGGACAAGTCAT3550.12539650020134085No Hit
CCTGAATGCCCCAACAGCTCTTTCTTAAACCTTCACCTACACGCCCTGCA3510.12398358188921306No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAACCCTTCACCTACACGCCCTGCA3500.12363035231118112No Hit
CGGTTCTTTGCAGTCAGGCCTGAGCCGCCTGGATACCGCAGCTAGGAATA3490.12327712273314918No Hit
CTCAGCAGAGATGCCTGATGGTTCAAGTAGAGGCCCAGTACATCTTGATC3440.12151097484298945No Hit
GTTTCCCATCAGCCGCCCAAACCTGGAGACCTGATTGAGATTTTCCGCCT3180.11232700581415886No Hit
GGGGAACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG3000.10596887340958382No Hit
GTTTCCCACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC2960.10455595509745604No Hit
CGATTCTCAGGGCGCCCAGTGAAGAAATCTATACGAGATACTCCAGCCAA2950.1042027255194241No Hit
CGATTCTCAGGGCGCCCAACGTCGCTCTTAGCTGAGTGTCCCGCGGGGCC2910.1027898072072963No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)