FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002271304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314499
Sequences flagged as poor quality0
Sequence length15-160
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA238867.59493670886076No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG188245.985392640358158No Hit
CAGACCCCAGGACCGGCCAGACTGTCCAGGCTACCGTCGGCGACAAGTGC107683.423858263460297No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA95583.039119361269829No Hit
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC90412.874730921242993No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG79522.5284659092715716No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA77842.4750476154137213No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA52101.6566030416630895No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATC49891.5863325479572272No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA47481.5097027335540019No Hit
GTTTCCCATCAGCCGCCCAAACCTCGACTGCGGTAGCAGACACAAGTGCG45961.4613718962540423No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC44281.407953602396192No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA41861.3310058219580985No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGG36531.1615299253733715No Hit
GCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCTGCAGA33191.0553292697274077No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT31691.0076343644971846No Hit
CGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCA27000.8585082941440195No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC26400.8394303320519303No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC26030.8276655887618084No Hit
CAGACCCCAGGACCGGCTTACACCCACGTATCAAGGTTGACCTCGGATCA24410.7761550911131673No Hit
CAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCCTTCTCA24390.7755191590434309No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC21340.6785395184086436No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG20170.6413374923290694No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG19840.6308446131784203No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC17330.5510351384265133No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC17030.5414961573804686No Hit
GTTTCCCATCAGCCGCCCAAACCTTAGAAAGCTGGATGATACAGACCCTG17000.5405422592758642No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC14910.47408735798841967No Hit
CGGTTCTTTGCAGTCAGGCCTGACGAGTGCTGACAGCTATGAAGAAGACC14570.4632765128029024No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA11440.36375314388916974No Hit
CAGACCCCAGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG11420.3631172118194334No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA11170.35516806094772957No Hit
CAGACCCCAGGACCGGCCGAACGCAGAGGTTGATTCTTCACCACACTGAA10450.3322745064372224No Hit
CCTGAATGCCCCAACAGCTCTTACTTATTCCTTCACCTACACACCCTGCA10390.33036671022801345No Hit
CAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC9790.3112887481359241No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA9500.3020677331247476No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT9330.296662310531989No Hit
TCTCACCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAG8720.2772663824050315No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA8590.2731328239517455No Hit
GATCCATCTCCACTCTGACGATTCAGCGCACAGAGCAGCGGGACTCAGCC8440.26836333342872315No Hit
CCTGAATGCCCCAACAGCTCAGGTCGCCCCGGAGTCGGCTCCTGCTGGGG8410.2674094353241187No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG8320.2645477410103053No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG8070.25659859013860137No Hit
CAGACCCCAGGACCGGCCCATGTCCCCCGGAGACTGTCCTCCGGAAACAA7390.2349768997675668No Hit
CAGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT7040.2238480885471814No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA6000.19077962092089323No Hit
CAGACCCCAGGACCGACCAAAGCCCGCGCGCCGCTGCATCCCGCGTCCAG5630.17901487763077148No Hit
TCTCACCTAAATCTCCAGACAACCTTAGCCAAACCATTTACCCAAATAAA5590.17774301349129884No Hit
GTTTCCCATCAGCCGCCCAAACCGTGGCAGCAGGACCGACAAGTCAAGTG5570.17710708142156256No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG5510.17519928521235362No Hit
CGGTTCTTTGCAGTCAGGCCTGACTGGCATTGTATTAGCAAACTCATCAC5090.1618447117478911No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG5060.16089081364328664No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT4970.15802911932947322No Hit
TCTCAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT4880.15516742501565983No Hit
CCTGAATGCCCCAACAGCCCTCCTGTGCCTGCTTCCCTGTGTGTCGGGGT4800.15262369673671458No Hit
CGATTCTCAGGGCGCTCTCCCAGCAAAGCAGTGGCCGCCCGGGCGGCGGG4500.14308471569066994No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG4040.1284582780867348No Hit
TCTCCACTCTGACGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC4020.12782234601699846No Hit
GATAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACAT3960.12591454980778952No Hit
GTTTCCCATCAGCCGCCCAAACCTGGAGACCTGATTGAGATTTTCCGCCT3870.12305285549397613No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC3730.11860133100582196No Hit
GGTACAGCGTCTCTCGGAACCCGTGCCAACGCTGCTGGTGATGCTATGAG3700.1176474329012175No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC3630.1154216706571404No Hit
CGGTTCTTTGCAGTCAGGCCTGTTATAACCGCTGAGCAGCCACACTCGCA3620.11510370462227223No Hit
CCTGAATGCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCAC3550.11287794237819516No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG3540.11255997634332701No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG3510.11160607823872254No Hit
CGATTCTCAGGGCGCCAGAGAGCTCTGGTGTTGCCCAGCAAATGAACGAT3440.10938031599464546No Hit
GCTGAAAGGACTGGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA3400.10810845185517282No Hit
GTTTCCCATCAGCCGCCCAAATCTAACATTCTCAACTCTGACTGTGAGCA3350.10651862168083205No Hit
CCTGAATGCCCCAACAGCAGCAACCCTATCATCGTCTCCTGTGGCTGGGA3290.10461082547162312No Hit
CGATTCTCAGGGCGCCCAACGTCGCTCTTAGCTGAGTGTCCCGCGGGGCC3260.10365692736701865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)