FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314499
Sequences flagged as poor quality0
Sequence length15
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN3254910.349476468923589No Hit
AGGCATGCCTTACGA57241.8200375835853215No Hit
TCTCGAAATGCCGTT50651.610497966607207No Hit
GGCACTTTTTTTAGT50211.5965074610730081No Hit
TCACTGAGTGTGCAT37581.194916359034528No Hit
TCACTGAGGGACTCT29880.9500825121860482No Hit
CTAGTGCAAGGGGGG29600.94117946320974No Hit
GGACTAAAGACGGTA26340.8375225358427213No Hit
ACTGCAGGGCACAGT24610.7825144118105304No Hit
TCAACGTCTGGTGAA24400.7758371250782992No Hit
GTAAGTCATGTTTGT23370.7430866234868791No Hit
TGCGTATGCTAAAGA23270.7399069631381976No Hit
CGGATTAGACGTTTT23010.7316398462316256No Hit
GGTTCAAAGGGATAG21240.675359858059962No Hit
GCATACTTTTTTCAT21000.6677286732231262No Hit
GTCATGCCGTGCATG19470.6190798698882986No Hit
CAGTTCAAGTGAGGG19120.6079510586679131No Hit
TGTCTCAGGAGGAGA18980.6034995341797589No Hit
TCAACGTGTAAGCAT18980.6034995341797589No Hit
GAGCTGAGGTGAGAT18050.5739286929370204No Hit
TAGACCATATATTTT17190.5465836139383592No Hit
TGCTACTTACCTGTC17010.5408602253107323No Hit
TACGACTGGACTATC16850.5357727687528419No Hit
AGCAGGTGTACCATT15070.47917481454631017No Hit
TACGGTCTTAGGGAT12360.3930060190970401No Hit
ATCGCCATGATCTTT12320.39173415495756747No Hit
TCGCCAAGTTCAGGT12130.38569280029507247No Hit
GCATCCATTTTTGGT11950.3799694116674457No Hit
CAGTTCATTCTTTAT11050.3513524685293117No Hit
GAGCTAGAGAATTTT10690.3399056912740581No Hit
GGACATTGCGTGTAT9870.31383247641486933No Hit
TACGACTGCGACAGT9860.3135145103800012No Hit
GGTTCAAGTCTTGCT9390.2985701067411979No Hit
GTTCAACATCTCCAT8790.2794921446491086No Hit
CTTCGAACGTAGGAA7550.24006435632545733No Hit
CGGTTATTTACGCGC7500.23847452615111653No Hit
CGCTTGATTGCATGT5700.18124063987484856No Hit
TCACTGAGGGTGCAT5400.1717016588288039No Hit
TCACTGAGTGGGCAT5260.16725013434064973No Hit
AACATCGTTAACTAT5220.1659782702011771No Hit
GGCGATTTAATAAGC5010.15930098346894583No Hit
ACAATCGAAGGCTCA4990.15866505139920953No Hit
GCACTGAGTGTGCAT4820.1532596288064509No Hit
CAATCGATGTCTGGA4540.14435657983014252No Hit
TTACTGCGCGACTTT4370.1389511572373839No Hit
GTCCATAGGCGGTTT4210.1338637006794934No Hit
AATGCCGGTAGACCC4050.12877624412160293No Hit
GTCGAGACGCGTCCG3970.12623251584265768No Hit
CATGTGCTGGACACT3920.12464268566831692No Hit
GCACTGAGGGACTCT3870.12305285549397613No Hit
TCACTGAAGGGGGTG3820.12146302531963536No Hit
CGTTACGGATCTACT3780.12019116118016274No Hit
TTAGCGTCTAACTAT3720.1182833649709538No Hit
TGCACGTAGGCAAAC3680.11701150083148118No Hit
AGCAGGTCCTCACGT3570.11351387444793147No Hit
CTGATCATGTCGTGA3520.1119240442735907No Hit
CATGCTGGTACAGGG3520.1119240442735907No Hit
GGACGATCGTTATTT3480.11065218013411808No Hit
CTAGAGCAAGGGGGG3470.11033421409924991No Hit
CCATGACGAGCTGCG3460.11001624806438177No Hit
AGTGTACGCTTGTTA3440.10938031599464546No Hit
CATTGTCCCCATAGT3300.10492879150649129No Hit
TCACTTGTTATGATG3280.10429285943675495No Hit
CTAGTGCAAGGGGGT3260.10365692736701865No Hit
GCAGTAGTTGTTACC3220.10238506322754602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)