FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002271308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282141
Sequences flagged as poor quality0
Sequence length15-160
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA248208.797019929751436No Hit
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA181796.443232284566937No Hit
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC140084.9648934398049205No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA111433.949443717857384No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC108953.8615444051024137No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG91283.235261801723252No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG82922.9389560538879493No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA47251.6746945676098122No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA44761.5864408221421205No Hit
GCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCTGCAGA41631.4755033830602429No Hit
CAGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT33041.1710456828323426No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC31811.1274504591675794No Hit
CAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCCTTCTCA31651.1217795357640328No Hit
CAGACCCCAGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG27220.9647658440283405No Hit
CCTGAATGCCCCAACAGCCCTCCTGTGCCTGCTTCCCTGTGTGTCGGGGT23870.8460308852665867No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA22870.8105876139944213No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC20250.717726243261348No Hit
GTTTCCCATCAGCCGCCCAAACCTTAGAAAGCTGGATGATACAGACCCTG19900.7053210983160901No Hit
CAGACCCCAGGACCGGCCAGACTGTCCAGGCTACCGTCGGCGACAAGTGC18020.6386877483244193No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA17390.6163584874229552No Hit
CAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC17220.6103331313066871No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG15910.5639024459401505No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC15370.5447630794531813No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAGA15190.5383832906241914No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG14010.4965602305230364No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC13590.4816740565887269No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA12950.45899036297454116No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG11710.41504070659705605No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA11260.3990912345245816No Hit
CAGATGGCTACAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT11260.3990912345245816No Hit
CAACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAGTCT10760.38136959888849903No Hit
CGGTTCTTTGCAGTCAGGCCTGACGAGTGCTGACAGCTATGAAGAAGACC10280.3643568286778596No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC9540.33812880793645733No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA9280.32891355740569433No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA8680.3076475946423951No Hit
CCTGAATGCCCCAACAGCTCTTACTTATTCCTTCACCTACACACCCTGCA7860.2785841121992195No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA7500.26582453454124No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC7230.2562548512977554No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA7050.24987506246876562No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG6500.23038126326907468No Hit
TCTCAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT6010.21301406034571366No Hit
CAGACCCCAGGACCGGCTTACACCCACGTATCAAGGTTGACCTCGGATCA5890.20876086779305383No Hit
TCTCACCTAAATCTCCAGACAACCTTAGCCAAACCATTTACCCAAATAAA5800.20557097337855892No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG5760.20415324252767236No Hit
CGATTCTCAGGGCGCTCTCCCAGCAAAGCAGTGGCCGCCCGGGCGGCGGG5660.20060891540045583No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC5610.19883675183684754No Hit
CGATTCTCAGGGCGCCCAACGTCGCTCTTAGCTGAGTGTCCCGCGGGGCC5350.18962150130608454No Hit
GCTGAAAGGACTGGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA5320.1885582031679196No Hit
CTGCAGAGAGGCCTAAGGGATTCTTCTCCACCTTGGAGATCCAGCGCACA5120.18146954891348652No Hit
TCTCACCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAG5020.17792522178627No Hit
GATAACTTCCAATCCAGGAGGCCGGCACGGAGGTGGTCAAGGCTAAAGCC4810.17048213481911526No Hit
TCTCAGCTCAACAGTTCAGTGACTTGCACTCTGAACTAAACCTGAGCTCT4620.16374791327740384No Hit
CGGTTCTTTGCAGTCAGGCCTGACTGGCATTGTATTAGCAAACTCATCAC4590.1626846151392389No Hit
GTTTCCCATCAGCCGCCCAAACCGTGGCAGCAGGACCGACAAGTCAAGTG4320.15311493189575426No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGT4310.15276049918303258No Hit
CCTGAATGCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCAC4260.15098833561942432No Hit
GTTTCCCACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC4110.14567184492859953No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG3990.1414186523759397No Hit
GGGGTACAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG3850.13645659439783653No Hit
CAGACCCCAGGACCAGCGAGCGCAGGGGGCAGTGCTGAGGCCCTGCTGAG3790.13432999812150662No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTG3770.13362113269606332No Hit
CGATTCTCAGGGCTGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGC3730.1322034018451767No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG3680.13043123828156844No Hit
GTTTCCCATCAGCCGCCCAAACCCGGGGGCTGCTCAGTCACGAGCCTTTC3660.12972237285612515No Hit
TCTCAGCTCAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCT3580.12688691115435188No Hit
CTCAGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA3570.12653247844163024No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCA3550.12582361301618694No Hit
CTCAGCAGAGATGCCTGATGGTTCAAGTAGAGGCCCAGTACATCTTGATC3540.1254691803034653No Hit
CAGACCCCAGGACCGGCCGAACGCAGAGGTTGATTCTTCACCACACTGAA3370.11944382418719718No Hit
CGGTTCTTTGCAGTCAGGCCTGAGCCGCCTGGATACCGCAGCTAGGAATA3360.11908939147447553No Hit
CGATTCTCAGGGCGCCCAACGATCTCAACCTATTCTCAAACTTTAAATGG3170.1123551699327641No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG3140.11129187179459915No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT3040.10774754466738262No Hit
GCAGAGAGGCTCAAAGGAGTCAACATTTAGATCTGGAAGGGACAAGTCAT2990.10597538110377436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)