FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311223
Sequences flagged as poor quality0
Sequence length15
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN5554217.846367395725895No Hit
TAGGTGCGTTAGATT25260.8116366720968566No Hit
GATGCTCTTATTTCG21460.68953772696748No Hit
GCGTTAGTGGGGTTA18350.58960937976949No Hit
TACTAGCTCATTCCT17500.5622977736221294No Hit
TAGGTGCTATTTGTT13400.43055943808780206No Hit
GTCACACATGCATTT11070.3556935059426842No Hit
TTGCCAGGTTTTTAT10760.34573280252423505No Hit
AACTCGTTCAGAGGC10500.33737866417327766No Hit
TAGGTGCTATGATGT10120.32516876966034003No Hit
AAGACTCAAATGCTG9750.3132801881609007No Hit
AGGCATGGAATGAAT9480.30460473679644495No Hit
GAGACTAAGGCGATT9140.29368009433750075No Hit
TATGTCCGGCGTGCT8810.2830767648920549No Hit
CGTTACGCAGGCCCG8450.27150949640611394No Hit
GCATGGACGACGAAG8210.2637979840821533No Hit
ACGGTAGGGTCTGGG8170.2625127320281599No Hit
GCTACCAAGAGCTGT8050.2586569758661796No Hit
GTCACTAATTAGGTA7900.25383728066370415No Hit
TGCCAGAAGCTTCAC7790.2503028375152222No Hit
TACGACTCGGTTATC7370.2368076909482911No Hit
TCACAGTGTGTGACA7240.23263062177281243No Hit
ATGACGTGTTGGTTT7230.23230930875931408No Hit
TGGACACCTCTATTT6820.2191354752058813No Hit
ATTGACGCGGGATTA6620.2127092149359141No Hit
GCATCCAATGCAAAT6210.19953538138248136No Hit
CATGCTGCTTGGGGG6040.19407306015300926No Hit
ATGCGCACTGGGTTC6030.1937517471395109No Hit
GAATAGCCCTGAAGT5430.17447296632960932No Hit
CGAAGGTAGAGCTGT5000.16065650674917986No Hit
CAGCGTAATAATTTG4860.1561581245602028No Hit
TCGAAGGATATCGGA4840.1555154985332061No Hit
CTACGATACGTTTTT4750.15262368141172086No Hit
CTACGTAGCGTCGGT4680.15037449031723235No Hit
TAGGCGCGTTAGATT4650.14941055127673725No Hit
ATAGGTCGGTATAGA4550.14619742114175366No Hit
CAGCGTAGTCATGCT3810.12242025814287504No Hit
TCGAAGGGATGGAGT3670.117921875953898No Hit
TAGTGGCTAAACCCC3570.11470874581891441No Hit
CAACTGATGTGCCTG3460.11117430267043246No Hit
GGACATTCGGTACGT3440.11053167664343574No Hit
GCTACCATTCCGTTT3220.10346279034647182No Hit
TGACACATTAGATAT3140.10089228623848495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)