FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407716
Sequences flagged as poor quality0
Sequence length15
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN383039.394529525453994No Hit
ACGTCAATACGTGGG123053.018032159640534No Hit
AACTCGTCGGTTTTG44151.0828615997409963No Hit
GCCATAGGGATCGCC26840.6583013666375614No Hit
AGATCACCTGTGGCT20920.5131022574537178No Hit
TCACTGAGGCTCGTG20030.4912733373230386No Hit
AACATCGCATGCTTG19850.48685849954380006No Hit
GTTCATGGACAGGAC18930.46429377311658115No Hit
AAGACTCGCGTAGCG15430.3784497051869438No Hit
ATGTCGCCATACCAC15170.37207271728359936No Hit
GCAGTAGCGTTCTTT14370.3524512160425394No Hit
CGCATATATAGGTCG13260.3252263830705687No Hit
CTGATCAAGCTGGGT13100.3213020828223567No Hit
ACCATGTACTTGATT12540.30756703195361473No Hit
GGCACTTTCAGCTTT11220.27519155490586583No Hit
AGATCACCAGTTTTT10850.26611661058187563No Hit
GAGACTACAATGATT10070.24698564687184216No Hit
TACGAGGTTCCGTAG9870.24208027156157716No Hit
TCGACTCCGGTCATT9170.2249114579756497No Hit
CGCTACAATAAAATT8470.20774264438972226No Hit
AGCCGATCTAGACAT8200.20112038772086452No Hit
GCTCATATGGAAGAT7800.19130963710033455No Hit
GATGCTCATAGTGGT7490.1837063053694238No Hit
GGTCTTAGATTAGCC7140.1751218985764601No Hit
CACAGATAGCAAGGT7010.17193340462478784No Hit
TACCATGCGTTATCT6610.16212265400425788No Hit
TCTCATGAAGAGTGG6590.16163211647323136No Hit
ATGTCGCCATTAAAT6100.14961394696308214No Hit
AACAGCTGGTCAGAG5890.1444633028873039No Hit
CTTGTACGTGCAGAC5820.14274642152871114No Hit
GCTCTAAGAAGTGGC5820.14274642152871114No Hit
AGTACCTCGAAGTAT5740.14078427140460517No Hit
GTGTCTAGCCCTGTT5500.13489782103228717No Hit
AGTCAGTGCCGGTCC5320.1304829832530487No Hit
ACGATGCAAACCGGA5310.13023771448753546No Hit
ATTCCAGGTTGGGCC5300.1299924457220222No Hit
CCTGATGTAGTCACT5270.12925663942548246No Hit
GATGCTCATGTTTTG4900.12018169510149221No Hit
AGTCAGTGTTTCGCC4880.11969115757046571No Hit
TCGTAGCAGTAGTGA4470.10963513818442248No Hit
GGTCTTAGGTAATAG4210.103258150281078No Hit
GGTCTTATTAGTGAT4130.101296000156972No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)