FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155901
Sequences flagged as poor quality0
Sequence length15
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN1567810.056381934689322No Hit
ACCAGTGATGTTTGT76104.881302878108543No Hit
TAGCGCACCGCGGGT17141.0994156548065759No Hit
GCGTTAGACGACATG16551.0615711252653928No Hit
GTAACTGCTTTCGGC12930.8293724863855909No Hit
TCGACTCAATAGGGC11620.7453448021500825No Hit
TCGACTCGTTATTCA10890.6985202147516694No Hit
CGTTGAAGCAGGCGT8230.527899115464301No Hit
CATTGGATCTAAGCC8170.524050519239774No Hit
TTCAGTCGTGGTTTG8140.5221262211275104No Hit
CCCAGTGATGTTTGT7760.4977517783721721No Hit
TATCCGCAATGGGGG5830.3739552664832169No Hit
TCGACTCTCTAAAGT5690.364975208625987No Hit
TCTAGTCGATGGTAG5530.35471228536058136No Hit
CAGCGATAGCGGGTG4630.29698334199267484No Hit
CAAGTTGGCGACGGA4460.2860789860231814No Hit
ACCCGTGATGTTTGT4350.279023226278215No Hit
ACCAGTGATGGTTGT3500.2245014464307477No Hit
ACTACTGTGGGCGTC3450.22129428291030848No Hit
GCGACATGGCTCTAT3360.2155213885735178No Hit
GGACTTCTGAGAAGA3300.2116727923489907No Hit
TTACTGCATACCATT2940.18858121500182806No Hit
ACCAGTGATGTTTGG2870.18409118607321315No Hit
TAGCGCACCGCGGGG2790.17895972444051034No Hit
ACCAGTGATGTTTTT2640.16933823387919258No Hit
TCTCGAAGGAGGTAT2590.16613107035875332No Hit
TGACGTCTCATGGGA2580.16548963765466546No Hit
GAAACTGCTTTCGGC2470.1584338779096991No Hit
CAGTCAAGGACTCTC2240.14368092571567856No Hit
CGAGTGCTTGTTCAA2170.1391908967870636No Hit
TACGGTCCCTTACGT2140.13726659867480004No Hit
CATTGGCTCTAAGCC2130.13662516597071217No Hit
ACCAGTGCTGTTTGT2030.13021083892983368No Hit
CAGCGCACCGCGGGT1990.12764510811348226No Hit
CATTGGAGCTAAGCC1910.12251364648077946No Hit
CTCATGAATAAGTTT1900.1218722137766916No Hit
ACCAGTGACGCTTTT1880.12058934836851593No Hit
ACCAGTGGTGTTTGT1880.12058934836851593No Hit
TACTAGGCGGCAAGT1850.11866505025625237No Hit
ACCAGTGAGGTTTGT1850.11866505025625237No Hit
GGTCTTAGCGTACAC1840.1180236175521645No Hit
CTCGATCATGGGTGG1820.11674075214398881No Hit
CCGACTCGTTATTCA1720.1103264251031103No Hit
ATGGCTCGCCAATGG1630.10455353076631965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)