FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002271320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190636
Sequences flagged as poor quality0
Sequence length15-160
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA2716814.251243206949368No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG147217.722046203235486No Hit
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC121536.374976394804759No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG46562.442350867622065No Hit
GTTTCCCATCAGCCGCCCAAACCTTAGAAAGCTGGATGATACAGACCCTG40342.1160746133993578No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA39832.089322058792673No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG32431.7011477370486163No Hit
CAGACCCCAGGACCGGAGGACGAGGACTCCAGCCTGGATGAATCTGACCT27681.4519817872804717No Hit
GCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCTGCAGA27581.4467361883379846No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC22921.2022912776180785No Hit
GATCCATCTCCACTCTGACGATTCAGCGCACAGAGCAGCGGGACTCAGCC22141.161375605866678No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC21881.1477370486162108No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC14720.7721521643341236No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG10420.5465914098071718No Hit
CGATTCTCAGGGCGCTCTCCCAGCAAAGCAGTGGCCGCCCGGGCGGCGGG9280.48679158186281707No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA8940.4689565454583604No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC8860.46476006630437067No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA8820.46266182672737577No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG8210.43066367317820353No Hit
CAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCCTTCTCA8040.4217461549759751No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA7040.3692901655511026No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA6910.3624708869258692No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA6390.3351937724249355No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG6370.334144652636438No Hit
CGGTTCTTTGCAGTCAGGCCTGTTATAACCGCTGAGCAGCCACACTCGCA6230.3268008141169559No Hit
CCTGAATGCCCCAACAGCCCTCCTGTGCCTGCTTCCCTGTGTGTCGGGGT6020.31578505633773263No Hit
TCTCAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT5930.3110640172894941No Hit
CCTGAATGCCCCAACAGCTTCATCCTGGGTCTGAAGCAGCAGATTGAAGA5860.30739209802975304No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC5770.30267105898151453No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG5750.30162193919301705No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT5570.29217986109654No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC5380.2822132231058142No Hit
CAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC5160.2706729054323423No Hit
GTTTCCCATCAGCCGCCCAAACCGTGGCAGCAGGACCGACAAGTCAAGTG5080.26647642627835244No Hit
CCTGAATGCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCAC5020.2633290669128601No Hit
CGATTCTCAGGGCGCCCAACGATAGAAGATTCCTACAGAAAGCAAGTTGA4990.26175538723011393No Hit
TCTCACCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAG4920.25808346797037285No Hit
GCAGAGAGGCTCAAAGGAGTCAACATTTAGATCTGGAAGGGACAAGTCAT4910.25755890807612414No Hit
CAGACCCCAGGACCCATTAAGCCACCCCCGTGTTCCTGCCGTCAGTGCCA4710.24706771019114962No Hit
CGATTCTCAGGGCGCCGCAGCCTGACGAGCTGCCCGAGGTGGATAACCTG4520.23710107220042384No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC4400.23080635346943915No Hit
CGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCA4390.23028179357519044No Hit
CCTGAATGCCCCAACAGCAGCAACCCTATCATCGTCTCCTGTGGCTGGGA4100.21506955664197738No Hit
CAGACCCCAGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG3980.20877483791099266No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC3810.19985731970876436No Hit
CAGACCCCAGGACCGGCCGAACGCAGAGGTTGATTCTTCACCACACTGAA3800.19933275981451562No Hit
GATCCATCTCCACTCTGACAGTGACCAGTGCCCATCCTGAAGACAGCAGC3430.17992404372731277No Hit
CAGATGGCTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT3360.1762521244675717No Hit
CTGCAGAGAGGCCTAAGGGATCCATCTCCACTCTGACGATTCAGCGCACA3330.17467844478482553No Hit
CAGATGGCTATAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT3240.169957405736587No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC3170.1662854864768459No Hit
GATAACTTCCAATCCAGGAGGCCGGCACGGAGGTGGTCAAGGCTAAAGCC3110.1631381271113536No Hit
GGACAAGTTTCTCATCAACAACCGACTAATCACCACCCAACAATGACTAA3090.16208900732285614No Hit
CAGACCCCAGGACCAGCGAGCGCAGGGGGCAGTGCTGAGGCCCTGCTGAG2980.15631884848612013No Hit
CGATTCTCAGGGCTGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGC2890.15159780943788162No Hit
GTTTCCCATCAGCCGCCCAAACCAGGGCTATGAAGCAGGTAGCAGGTACC2730.143204851129902No Hit
CAGACCCCAGGACCGCGCCCCCAGAGATTTCTGCGTCAAGTTCCCTGAGG2670.14005749176440968No Hit
TCTCACCTAAATCTCCAGACAACCTTAGCCAAACCATTTACCCAAATAAA2650.1390083719759122No Hit
GGGGAACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG2530.1327136532449275No Hit
CAGACCCCAGTCCACCATGATCCATCTGGGTCACATCCTCTTCCTGCTTT2450.1285171740909377No Hit
CTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATTCAGCGCACA2440.12799261419668898No Hit
CGATTCTCAGGGCGCCAGTTCCCGAACAAAGATGCCCTAGTCAGGCACCA2360.12379613504269918No Hit
GTTTCCCACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC2260.11855053610021192No Hit
NNNNNNNNNNNNNNN2240.11750141631171446No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGC2210.11592773662896831No Hit
CGGTTCTTTGCAGTCAGGCCTGACTGGCATTGTATTAGCAAACTCATCAC2200.11540317673471957No Hit
GGTACAGCGTCTCTCGGGACAACTGGCACAAGCGCCGCAAAACCGGGGGC2190.11487861684047085No Hit
CAGACCCCAGGACCAGCTGACAGCACTCACTGGGCGGATCCAGGAGGCCG2180.11435405694622212No Hit
CGATTCTCAGGGCGCCCAGTGAAGAAATCTATACGAGATACTCCAGCCAA2140.11225581736922723No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTG2130.1117312574749785No Hit
CGATTCTCAGGGCGCCCACGATGCTCTTAGCTGAGTGTCCCGCGGGGCCC2010.10543653874399378No Hit
CGATTCTCAGGGCGCCAGTCAGCTTTAAGAAAAAGTAGAGATCACTTCTG2010.10543653874399378No Hit
TCTCCACTCTGACGATTCAGCGCACAGAGCAGCGGGACTCAGCCATGTAT1960.10281373927275017No Hit
CCTGACTGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA1930.101240059590004No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA1930.101240059590004No Hit
TGGCTACAATGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG1910.10019093980150653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)