FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190636
Sequences flagged as poor quality0
Sequence length15
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN171949.019282821712583No Hit
ACACGTGGTTTACGC118706.226525944732369No Hit
TACTGACCCACCCAC33291.7462598879540066No Hit
CGTAACTGGGAATGC27861.461423865376949No Hit
AGCTGGTTGTCAGAT18410.9657147653119033No Hit
CACGTATCTGCGTGG12710.6667156255901299No Hit
TAGCCGAAGTGAGTC8760.4595144673618834No Hit
CTTAGTGATGTGCTC8160.4280408737069598No Hit
TACGAGGTGATGCAC6710.3519796890408947No Hit
CCATGGTGCGCGGGG6220.3262762542227072No Hit
ATCTACGAAAGTGGT6150.3226043349629661No Hit
ACACGTGTTTTACGC6100.3199815354917224No Hit
GTGTCTATAAGGTTA5560.2916553012022913No Hit
CTTGTACCGCGGCTG5540.2906061814137938No Hit
AAGGTACAACCAAGC5390.2827377830000629No Hit
ACACGTTGTTTACGC5380.2822132231058142No Hit
TGCTGGTTGTCAGAT4520.23710107220042384No Hit
ATCAGGCGGTGTAAT4490.23552739251767765No Hit
GCTTGTAGGCGTGAG4070.2134958769592312No Hit
AGGTCTGTTGTTGGC4070.2134958769592312No Hit
ACACGTGGGTTACGC3940.2066765983339978No Hit
CATGAACGCCTGGTC3830.2009064394972618No Hit
TCTTCGAGAATTTAT3690.19356260097777964No Hit
CTACAAGATTCCTTT3480.1825468431985564No Hit
CGTGCATACTCGGTT3420.17939948383306406No Hit
CGCTTGATGGGTGTA3120.1636626870056023No Hit
TTGAAACCATTTCTT2680.1405820516586584No Hit
TCACGTGGTTTACGC2680.1405820516586584No Hit
ATCTGCATCCAATAT2560.13428733292767367No Hit
TTAGCGTAGTAATCT2530.1327136532449275No Hit
AGTACCTGCGCAGAG2450.1285171740909377No Hit
TAGCCGAATTATGCT2430.12746805430244026No Hit
TACGTACGGCCGGGG2330.12222245535995299No Hit
CTTGTACTGAACTTT2300.12064877567720682No Hit
ACACGCGGTTTACGC2270.11907509599446064No Hit
CGATATCGGCGCGCG2220.11645229652321704No Hit
GATAGTCGGGAAAAG2200.11540317673471957No Hit
ACACGTGGTTTACTC2140.11225581736922723No Hit
ACACGTGGATTACGC2090.10963301789798359No Hit
AGGTCTGTTATTAGT2000.10491197884974506No Hit
CAGCACTTCAGAATG1930.101240059590004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)