FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175615
Sequences flagged as poor quality0
Sequence length15
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN2297413.08202602283404No Hit
TAGCGCAGTCTGGGT143908.194060871793411No Hit
CGGTATTGACGACTT37442.131936338012129No Hit
CAGCGTAGTTGTTTT22941.3062665489849956No Hit
ATAACCGTGGATACT17821.0147196993423113No Hit
AGATCGAGATTATCA12740.7254505594624605No Hit
CTGGAAGGATTAGCT9240.5261509552145317No Hit
GTAAGTCAACATGAA7480.4259317256498591No Hit
GGTATCAATCTTGTT6930.39461321641089886No Hit
CTGGAAGTCACGGTA5800.3302679156108533No Hit
TCACAAGATTGCGTG5570.3171710844745608No Hit
GCTGACATATAGCTT5560.31660165703385246No Hit
GGTATGCTTGTCGTT5340.3040742533382684No Hit
TAGCGCAGTCTGGGG4980.2835748654727671No Hit
TGCGTATGTAGAGAT3710.21125758050280444No Hit
CATGGATTTAGACGC3370.19189704751871994No Hit
GACCTAAGATGTAAG3240.18449449078951113No Hit
TGCTGAGGGAATTGA3160.1799390712638442No Hit
TGCGTATGTGGGGGG3110.17709193406030235No Hit
AGATCGACACTGGTT2860.1628562480425932No Hit
CAATCGAGAGCGTGT2780.15830082851692623No Hit
GACCTGTGTCTAAGC2690.15317598155055093No Hit
CATCTGGCGGAACCG2660.15146769922842582No Hit
GTCCAAGAGCTAATT2650.15089827178771745No Hit
GACATCCTAGAGCGT2560.14577342482134215No Hit
GTAATCCAAGAGAGT2350.1338154485664664No Hit
CTATGCCGGTTCCCG2330.1326765936850497No Hit
TATCGCGTTTTCCTG2310.13153773880363293No Hit
AGCACTGATTGGGTT2230.126982319277966No Hit
ATAACCGCGGATACT2160.12299632719300743No Hit
GACGTAGCCATGTGT2070.11787148022663213No Hit
TAGCGCAGGCTGGGT2020.11502434302309028No Hit
TTAACCGTGGATACT1990.11331606070096517No Hit
GACATCCGGTTTAAC1910.10876064117529825No Hit
AAGACTCTATAATGA1790.10192751188679783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)