FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002271324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193414
Sequences flagged as poor quality0
Sequence length15-160
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA4617023.87107448271583No Hit
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC98825.1092475208619845No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC86884.491918889015273No Hit
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA78364.051413031114604No Hit
GATAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACAT76673.964035695451208No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA75883.923190668720982No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA64803.350326243188187No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG56402.916024693145274No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA49452.5566918630502444No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA28401.4683528596688966No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG16940.8758414592532081No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC13280.686610069591653No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC9990.5165086291581789No Hit
CAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCCTTCTCA9910.5123724239196749No Hit
CAGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT9880.510821346955236No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC8530.441022883555482No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC7820.4043140620637596No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATC7420.3836330358712399No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG6840.3536455478920864No Hit
CTCCGCACAACAGTTCCCTGACTTGCACCCTGAACTAAACCTGAGCTCTC6300.32572616253218484No Hit
GTTTCCCATCAGCCGCCCAAACCTTAGAAAGCTGGATGATACAGACCCTG6110.315902675090738No Hit
CGATTCTCAGGGCGCTCTCCCAGCAAAGCAGTGGCCGCCCGGGCGGCGGG5820.30090893110116124No Hit
TACAAAGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATC5770.2983238028270963No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC5660.29263652062415335No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA4990.25799580175168296No Hit
GTTTCCCATCAGCCGCCCAAACCGTGGCAGCAGGACCGACAAGTCAAGTG4860.251274468239114No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT4570.23628072424953725No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG4520.23369559597547232No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG4460.23059344204659438No Hit
TCTCAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT4230.21870185198589556No Hit
CAGATGGCTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACT3880.2006059540674408No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC3710.19181651793561996No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA3630.18768031269711605No Hit
CAGACCCCAGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG3620.18716328704230303No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGG3420.1768227739460432No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT3400.17578872263641723No Hit
CAGATGGCTACAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT3200.16544820954015738No Hit
TCTCACCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAG3140.16234605561127943No Hit
TGGCTACAATGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA3080.1592439016824015No Hit
GATAACTTCCAATCCAGGAGGCCGGCACGGAGGTGGTCAAGGCTAAAGCC3050.1576928247179625No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA2960.15303959382464558No Hit
CAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC2820.1458012346572637No Hit
GGGGTACAGTGTCTCTAGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA2780.14373313203801172No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC2770.14321610638319873No Hit
CAGACCCCAGGACCAGCTGACAGCACTCACTGGGCGGATCCAGGAGGCCG2630.13597774721581685No Hit
TCTCCACTCTGACGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC2530.13080749066768693No Hit
GGTACATCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA2500.12925641370324795No Hit
CGATTCTCAGGGCTGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGC2470.127705336738809No Hit
CCTGAATGCCCCAACAGCCCTCCTGTGCCTGCTTCCCTGTGTGTCGGGGT2450.126671285429183No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGGGACA2410.12460318280993103No Hit
CCTGAATGCCCCAACAGCTAATCAGTAGAGACCTGGCGAGCATTTTACTT2300.11891590060698812No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG2240.11581374667811017No Hit
TCTCACCTAAATCTCCAGACAACCTTAGCCAAACCATTTACCCAAATAAA2190.1132286184040452No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG2140.11064349012998025No Hit
CGATTCTCAGGGCGCCCACGATGCTCTTAGCTGAGTGTCCCGCGGGGCCC2130.11012646447516726No Hit
CCTGAATGCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCAC2000.10340513096259837No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)