FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193414
Sequences flagged as poor quality0
Sequence length15
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTACGCGGTCAT3841719.862574580950707No Hit
NNNNNNNNNNNNNNN142837.384677427693963No Hit
ATGCTACGCGGGCAT10050.5196107830870568No Hit
CTAGAGTTTGCGGCT9280.4797998076664564No Hit
AGCTGTTAGATATGG9090.4699763202250096No Hit
AGATCACAGCTCTGT8660.44774421706805095No Hit
AGCTCAATGGGCATG8350.43171642176884817No Hit
TAACAGCTTTTTGAT7780.40224595944450764No Hit
ATGCGACGCGGTCAT7230.3738095484297931No Hit
CTGTGGACCAGGCGT6730.3479582656891435No Hit
ATGCTACGCGGACAT6580.3402028808669486No Hit
ATTCAGCTCTGTGTC6570.3396858552121356No Hit
TATAGGCTGTAATTT5900.3050451363396652No Hit
TCTACAGGCCAACGC5170.26730226353831676No Hit
ACTGTCCTGTTGGTT5120.26471713526425183No Hit
ACTCTTGAATACCCG5100.2636830839546258No Hit
TTGCTACGCGGTCAT5040.2605809300257479No Hit
CTGTGGATGTGTAAA4720.24403610907173215No Hit
CTGATGGGTTTGGGG4460.23059344204659438No Hit
TCAAACGTCGCTGGG4290.2218040059147735No Hit
TACGTACCGAAATTT4170.21559969805701756No Hit
CTAGAGTTACCGGCT4030.2083613388896357No Hit
ATGCTACTCGGTCAT4010.2073272875800097No Hit
ATTCTACGCGGTCAT3530.18251005614898613No Hit
ATGCTACGCGTTCAT3430.1773397996008562No Hit
ATGCTACGCGGTCCT3430.1773397996008562No Hit
ATGCTACGCTGTCAT3420.1768227739460432No Hit
CGATATCGCAGATCT3410.17630574829123022No Hit
CTAGAGTGGCGACTT3130.16182902995646645No Hit
CTGATCACTTAGTGA3070.1587268760275885No Hit
ATGCTACGCGGTCAG2760.14269908072838572No Hit
ATGCTACGCGGCCAT2640.13649477287062983No Hit
TCAACGTTTAGTCAT2600.13442667025137786No Hit
CTAGAGTCCTCTGGC2580.1333926189417519No Hit
ATACTACGCGGTCAT2520.13029046501287395No Hit
CGTATGAATGGGTGT2500.12925641370324795No Hit
GAGCTGACGCTGTAA2210.11426266971367119No Hit
TTGGTCAAATCTTGA2080.1075413362011023No Hit
TATCGCGCTGCGCGT2060.10650728489147632No Hit
CTGCTACGCGGTCAT2060.10650728489147632No Hit
TGCTGTTAGATATGG2000.10340513096259837No Hit
TCCGCATGCTTGGGT1980.10237107965297239No Hit
ACGCTACGCGGTCAT1960.1013370283433464No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)