FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002271328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178042
Sequences flagged as poor quality0
Sequence length15-160
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA3033217.036429606497343No Hit
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA2286612.84303703620494No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA99795.604857280866312No Hit
CAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCCTCCTCA72974.098471147257389No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC69733.91649161433819No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA49592.7852978510688486No Hit
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC48292.712281371811146No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG47142.6476898709293315No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA34111.915840082677121No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA26801.5052628031588051No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG26241.473809550555487No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC25291.4204513541748576No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA21711.2193752036036443No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA18321.0289706923085564No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGG16660.9357342649487199No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC10790.606036777838937No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG9420.5290886420058188No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAGA8040.4515788409476416No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGC6470.3633974006133384No Hit
CGATTCTCAGGGCGCTCTCCCAGCAAAGCAGTGGCCGCCCGGGCGGCGGG6320.35497242223744957No Hit
GTTTCCCATCAGCCGCCCAAACCGTGGCAGCAGGACCGACAAGTCAAGTG5850.3285741566596646No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG4690.2634209905527909No Hit
CGATTCTCAGGGCGCCATCGCCAAGTAAGAAAGTGAAGCAAATCAGAAAC4110.23084440749935406No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA3980.22354275957358377No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC3950.221857763898406No Hit
CCTGAATGCCCCAACAGCCCTCCTGTGCCTGCTTCCCTGTGTGTCGGGGT3950.221857763898406No Hit
CAACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAGTCT3920.2201727682232282No Hit
TACAAAGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATC3870.21736444209793193No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA3740.21006279417216164No Hit
CAGATGGCTACAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG3470.19489783309556172No Hit
CAGATGGCTATAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG3300.18534952426955437No Hit
GCAGAGAGGCTCAAAGGAGTCAACATTTAGATCTGGAAGGGACAAGTCAT3170.17804787634378405No Hit
CAGATGGCTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACT2600.14603295851540649No Hit
TCTCACCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAG2540.14266296716505095No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC2450.13760798013951764No Hit
CGATTCTCAGGGCGCCCAGTGAAGAAATCTATACGAGATACTCCAGCCAA2320.13030633221374732No Hit
TCTCAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT2260.12693634086339178No Hit
GATAACTTCCAATCCAGGAGGCCGGCACGGAGGTGGTCAAGGCTAAAGCC2120.1190730277125622No Hit
NNNNNNNNNNNNNNN2110.11851136248750295No Hit
GTGTCTCCAGAGCAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCT2060.11570303636220668No Hit
CCTGAATGCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCAC1980.11120971456173262No Hit
CCTGAATGCCCCAACAGCAGCAACCCTATCATCGTCTCCTGTGGCTGGGA1930.10840138843643635No Hit
CAACAGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCG1910.10727805798631783No Hit
TGGCTACAATGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA1890.10615472753619934No Hit
GGGGTACAGTGTCTCTAGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA1840.10334640141090304No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACACCCTGCA1810.10166140573572527No Hit
CAGACCCCAGGACCCATTAAGCCACCCCCGTGTTCCTGCCGTCAGTGCCA1800.10109974051066603No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATC1790.10053807528560676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)