FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178042
Sequences flagged as poor quality0
Sequence length15
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGTTCAGTGGTG2896716.269756574291456No Hit
NNNNNNNNNNNNNNN86134.837622583435369No Hit
CAGGAATCGGGATGT34471.9360600307792544No Hit
TGTCTCACGTGTAGG19281.0828905539142448No Hit
TCTAGTCGCATGTAG14360.8065512631850912No Hit
TGTGACTTTTTATGT12960.7279181316767954No Hit
TTGGTCAGTAAAAAT12270.6891632311477067No Hit
GCTTAGATGGGCTGG10460.5875018254119815No Hit
TCTCATGTAGACCGG10340.5807618427112703No Hit
AGATCACGGATTATC9940.5582952337089001No Hit
CGAGATTCTGGAGGC8090.45438716707293786No Hit
ACGGTAGATGACTAG8030.4510171757225823No Hit
GCTCTAAACGGTTAT7720.4336055537457454No Hit
TTAGCGTCAATCCGT6940.38979566619112344No Hit
AGGCGTTCAGTGGGG6730.3780006964648791No Hit
GTCACACACAAATCT6560.3684523876388717No Hit
CGAGTGAATCCGTCG6120.3437391177362645No Hit
TCTAGTCTGAAGTAT5950.33419080891025715No Hit
TCTCGAAGCCTTAGC5890.33082081755990156No Hit
AGGCGTTCAGGGGTG5390.3027375563069388No Hit
AGGCGATCAGTGGTG5370.3016142258568203No Hit
AGGACATGGTTTACT4830.2712843037036205No Hit
TGCACAAATGGATTT4510.25331101650172433No Hit
AGGCGTTCAGTGGAG3780.21230945507239868No Hit
CTATGCCGCTCTCCG3760.21118612462228012No Hit
AGGCGTGCAGTGGTG3360.1887195156199099No Hit
GCCATAGTGCTTGAA3290.18478785904449513No Hit
AGATCACCGAAAGTG2890.1623212500421249No Hit
GAATAGCTCCTTGCG2800.15726626301659158No Hit
AGGCGGTCAGTGGTG2780.1561429325664731No Hit
GAATAGCGGGGCCGA2610.14659462374046572No Hit
TAGGAATCGGGATGT2600.14603295851540649No Hit
TGCACAATGGAGTCC2570.14434796284022872No Hit
AACCTTGCCTAGGTC2550.14322463239011018No Hit
TCTCTAAACGGTTAT2550.14322463239011018No Hit
TCTTAGATGGGCTGG2510.14097797148987318No Hit
ATGCCAAAGTGTCTG2510.14097797148987318No Hit
CAATCGAGTGGAAAC2280.1280596713135103No Hit
CAGGAATCGGGATGG2140.12019635816268072No Hit
AGGCGTTCAGAGGTG2090.11738803203738445No Hit
CAGGAAGCGGGATGT1970.11064804933667337No Hit
TCTAGACGCATGTAG1930.10840138843643635No Hit
CATCCGTATGCGAAG1890.10615472753619934No Hit
TTGGGCAGTAAAAAT1880.10559306231114006No Hit
AACGTTCGGGTTGGC1870.10503139708608082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)