FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352077
Sequences flagged as poor quality0
Sequence length15
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN297778.457524916424532No Hit
GCTTCATGGGGTGAT53741.526370651874448No Hit
ATGAGCGTCCTAAGG20040.5691936707027156No Hit
CTTGCCTGATTTTTT18320.5203407209218438No Hit
TAGAGCCTGTTAGCG15580.4425168358058038No Hit
GCTGAATATCGGGAT14600.4146820155818187No Hit
GTAATCCGTAAAAAC14230.4041729508033754No Hit
ATCAGGCGATGCGAG12830.3644089219119681No Hit
GACCTGTGTCTAAAT11870.3371421592435746No Hit
CGTCAGAACGGACCG11670.3314615836876592No Hit
ACTCAGGTAATAGTA11240.3192483462424413No Hit
GAGCTGACTAATGGG11230.3189643174646455No Hit
GCCATAGTTTATCTT10590.3007864756857165No Hit
CTATGCCGCTTGGTC10480.297662159129963No Hit
TAGCGTGCTTTCAGT10390.29510590012980115No Hit
CGTTGAAGAACGACG10020.2845968353513578No Hit
GCTTGTACAGCCACC10010.28431280657356206No Hit
AGCCGATGGCAGTAG9300.26414676335006265No Hit
TAGCGCAGGCCATAT9180.26073841801651343No Hit
ATCGTGATGGAGTGT9090.25818215901635155No Hit
ATGCCAATTTAGCAA8820.25051338201586587No Hit
GACCTGTGCGCCAGG8690.24682100790452088No Hit
ATGCCAAGGCTCGTC8440.2397202884596267No Hit
ATGCCAACTGTGTCT8290.23545985679269024No Hit
ATGCCAAAGACTTAT8060.22892719490338762No Hit
GACCTGTCCCTGTCT7680.2181341013471485No Hit
ATGCCAATCTTCGGG7170.2036486336795644No Hit
AACAGCTGTGAAAGT7090.2013764034571983No Hit
GGACTAGGCAAGGAG6910.19626388545687448No Hit
GCCTACATCTGGGGT6880.1954117991234872No Hit
CGTCCAAGGATGTGG6820.19370762645671258No Hit
GGATCTAGAACTCTT6760.192003453789938No Hit
ATGCCAAAGTTTTTC6310.17922215878912853No Hit
TAGCGCATCGGTAGA5650.16047625945460794No Hit
ATGCCAAGGCCAGGT5560.15792000045444604No Hit
AAGGCTAGTTCAGAT5460.15507971267648837No Hit
ATGCCAATGGGTGTG5100.14485467667584082No Hit
CGAATTCTGTGATCT5100.14485467667584082No Hit
CAGTCAACATGCGGT5030.14286647523127044No Hit
AGTCTCCTATCGTTT4950.1405942450089043No Hit
CCATGGTGGTTAAGG4850.13775395723094663No Hit
GCTTCATGGGGGGAT4790.13604978456417205No Hit
TCGTAGCTATGGTAG4720.13406158311960167No Hit
ATGCCAACGGGGGAA4620.131221295341644No Hit
CCATGGTAGATATAT4590.13036920900825671No Hit
TGACGGAGGACCACT4580.13008518023046095No Hit
CAGTCAACGTAACGT4310.12241640322997525No Hit
GGATCTAGGGAGCGG3990.11332748234051074No Hit
CTGTCATCGAAAGTC3860.10963510822916578No Hit
CACAGATTGGACAGG3800.1079309355623912No Hit
CAGTCAAATTACGGC3770.10707884922900389No Hit
AAGTGCATCGATGGG3760.10679482045120811No Hit
GGATCTATTTAGTTT3760.10679482045120811No Hit
GATGTACGGACGAGT3710.10537467656222929No Hit
ATGCCAACATGGTAG3680.10452259022884199No Hit
TCTCGAATTAGTCTT3680.10452259022884199No Hit
GTCCAAGCGAGGGTA3630.10310244633986315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)