FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002271332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691578
Sequences flagged as poor quality0
Sequence length15-160
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC253023.658589486652265No Hit
GCAGAGAGGCTCAAAGGAGTCAACATTTAGATCTGGAAGGGACAAGTCAT159972.3131158018329097No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC123791.7899644002556472No Hit
GTTTCCCATCAGCCGCCCAAACCGTGGCAGCAGGACCGACAAGTCAAGTG98861.4294844543927077No Hit
GTTTCCCATCAGCCGCCCAAACCTCTGCCTTAAACCTAGGTCACTCCACT76011.0990806532307273No Hit
CGATTCTCAGGGCGCCATCGCCAAGTAAGAAAGTGAAGCAAATCAGAAAC69381.0032129419964197No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG64320.9300469361373555No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC51840.7495900679315999No Hit
CAGACCCCAGGACCCACTCCTGAAGCTGCCTTCTCCAGGATGTGCCCTGG51590.7459751466935038No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG45780.6619643771201513No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG43580.630153070224906No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG42910.6204650813068086No Hit
GTTTCCCATCAGCCGCCCAAACCAGGGCTATGAAGCAGGTAGCAGGTACC38010.5496126250401256No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCA35680.5159215591010703No Hit
CAGACCCCAGGACCTTCCATATTTTTATCTAAGGTAAAGTGCTTCTCAAT35290.5102822819696404No Hit
TGGATACAGTGTCTCTCGACAGCAGAGTTGATAAAGCAGAAGAAAGAATA35260.5098484914210689No Hit
CCTGAATGCCCCAACAGCACAGACCTATGTATAACATTAGTTCCAGCAAG34750.5024740520953529No Hit
CGATTCTCAGGGCGCCCAGTGAAGAAATCTATACGAGATACTCCAGCCAA34410.49755775921154227No Hit
CGATTCTCAGGGCGCCCAACGATAGAGCTTACAGAAGAGAAAAAGGTATT31690.45822741614105716No Hit
CTGCAGAGAGGCTCAAAGGAGTCAACATTTAGATCTGGAAGGGACAAGTC31490.45533547915058026No Hit
GATAACTTCCAATCCAGGAGGCAGTGGAGGAAGTGGGGGTTATGGTGGGA31400.4540341075048657No Hit
CGATTCTCAGGGCGCCCAACGATCTGTAAAGGTGTCTTCCACGCTATAGT28350.40993206840009366No Hit
NNNNNNNNNNNNNNN26170.37840995520389603No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA25810.37320446862103773No Hit
CGATTCTCAGGGCGCCCAACGATAAAGAAAAGCACATATTTCAGTGATGA25710.37175850012579925No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG24970.3610583332610349No Hit
CAGACCCCAGGACCGGCCCTGCTGCTATGCTGACTTCTTTCCTCTCAAAC22900.3311267854095995No Hit
CGGTTCTTTGCAGTCAGGCCTGACGAGTGCTGACAGCTATGAAGAAGACC22530.3257767019772173No Hit
CGATTCTCAGGGCGCCAGCACACCTGGGTAATTAAGATTTTTTTTGTAGA20950.3029303997524502No Hit
GATAACTTCCAATCCAGGAGGCCGGCACGGAGGTGGTCAAGGCTAAAGCC20560.2972911226210203No Hit
CAGACCCCAGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG20410.2951221698781627No Hit
GGAACGATTTTCTGCTGAATTTCCCAACCCACCCCCAAACTAAACACTAA20320.2938207982324481No Hit
CGATTCTCAGGGCGCCAGTTCTCCAACAAGAGTTCTTAACCAGGCTGAAC20100.2906396675429236No Hit
GCTGAAAGGACTGGAGGGACATACTGGGTTCTCCTTCTTTTCGTAGACGT18830.27227586765339556No Hit
CCTGAATGCCCCAACAGCTCTGAAATATACACTCTTATCATCATGACTTG18390.26591360627434646No Hit
CCTGAATGCCCCAACAGCAGCAACCCTATCATCGTCTCCTGTGGCTGGGA17240.2492849685791046No Hit
CAGACCCCAGGACCGGCCTGGGAAGCTTTCAAGCAGATGTCTTTCTTCCT17070.24682682213719928No Hit
CAGACCCCAGGACCGCCACCTCTTCCTGTTCAACAGCATGATCCTTTACT15650.22629406950481365No Hit
CAGACCCCAGGACTGCAGGGGATTGAGCCCCAGCTTTGGGGCTGTGGACA15300.22123317977147916No Hit
CAGACCCCAGGACTGGTCACCTCATTCTGTGTTCAATCCGTTTCTAAGGA14860.21487091839243005No Hit
CAGACCCCAGGACCGACGGGAAGGTTTTCCAGTTTCTTAATGCGAAATGC14780.21371414359623933No Hit
CGATTCTCAGGGCGCCCAACGATAAACTCAAAAGTCTATGACAAATAGGG14450.20894244756195254No Hit
CGATTCTCAGGGCGCCAAGCAGTGACTTCTGACCAACAGAGTGTGGGGAG14340.20735188221719025No Hit
CCTGAATGCCCCAACAGCCATCAATTAATAAAGAGAATTATACATGGATA14070.2034477672800465No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG14070.2034477672800465No Hit
CCTGAATGCCCCAACAGCTCATTGAGAACGGGCCAGGATGACAATGGCGG13920.20127881453718888No Hit
CCTGAATGCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCAC13490.19506115000766364No Hit
CGGTTCTTTGCAGTCAGGCCTGACTGGCATTGTATTAGCAAACTCATCAC12410.17944469025908863No Hit
GGAACGATTTTCTGCTGAATTTCCCTCTGACAGAGGAAAAAAACCAACTT12260.177275737516231No Hit
CAGACCCCAGGACCGACCAAAGCCCGCGCGCCGCTGCATCCCGCGTCCAG12120.1752513816228972No Hit
CTCAGCAGAGATGCCTGATGGTTCAAGTAGAGGCCCAGTACATCTTGATC12110.17510678477337335No Hit
CAGACCCCAGGACCCAGCAGATGGGTTCAGTCCCAGAGCACTGGTGATCT12100.17496218792384952No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA12080.17467299422480184No Hit
TCTCACCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAG11620.16802153914670506No Hit
CTGCAGAGAGGCCCTGCTCATTATAGTCACACAAGGATTTAAGCTGATGG11490.16614178010289513No Hit
CTCAGCAGAGATGATAACATGTTTCAGATTGGGAAAATGAGGTACGTTAG11270.16296064941337057No Hit
CGATTCTCAGGGCGCAAAGGAACTGACATCATGTACACAGGCACGCTTGA11190.16180387461717985No Hit
CGATTCTCAGGGCGCCCAGAGAGGACCAGGGGCAAAGATAATTTTTTGTT10860.15703217858289303No Hit
TCTCACCTAAATCTCCAGACAAAGCTGGATTTGAATCCTGGCCTTACCTC10840.15674298488384536No Hit
GTTTCCCATCAGCCGCCCAAACCTTAGAAAGCTGGATGATACAGACCCTG10350.14965773925717707No Hit
CAGACCCCAGGACCAAGGCCAGAGCTAGACTGAGGCAGGCTCTGCTCCTA9730.14069273458669884No Hit
CCTGAATGCCCCAACAGCTGTGGAATACACATTTTTTCCAATAGCATGTG9640.13939136294098425No Hit
CGATTCTCAGGGCGCTCTCCCAGCAAAGCAGTGGCCGCCCGGGCGGCGGG9530.137800797596222No Hit
GGGGTACAGTGTCTCTCGACAGCAGAGTTGATAAAGCAGAAGAAAGAATA9430.13635482910098354No Hit
GCTGAAAGGACTGGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA9370.1354872480038405No Hit
CCTGAATGCCCCAACAGCCCTCCTGTGCCTGCTTCCCTGTGTGTCGGGGT9270.13404127950860206No Hit
GTTTCCCATCAGCCGCCCAAACCGCATTCACTCTAGTGTTTTTTAACCTG9080.13129393936764908No Hit
CAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC9000.1301371645714583No Hit
GGGGTACAGTGTCTCACGACTGTAATCCCAGCACTTTGGGAGGCAGAGGT8970.12970337402288679No Hit
GCTGAAAGGACTGGAGGGACAAGGATCTTAAACCCAAAGTACGAGAAATG8790.1271006307314576No Hit
CAGACCCCAGGACCAGCTGACAGCACTCACTGGGCGGATCCAGGAGGCCG8770.12681143703240993No Hit
CAGACCCCAGGACCACTCCGCCCCCGCTAACTCAATGCAGCTGACCCTTA8760.1266668401828861No Hit
GCTAAGATGCCTAATGCATCAGCTGCACCAAGGCAAAATCATTTTTGCCA8740.1263776464838384No Hit
GGGGTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG8690.1256546622362192No Hit
CGATTCTCAGGGCGCCCAACGATAGCAGAAGAAAGTAAATAACTAAAATC8670.1253654685371715No Hit
TGGATACAGTGTCTCTCGACAGGTGGGGCTGAGAAGAAAACTGAGCCAAG8640.12493167798859998No Hit
CCTGAATGCCCCAACAGCTCATTGAGAACGGGCCATGATGACGATGGCGG8550.1236303063428854No Hit
CCTGAATGCCCCAACAGCTTCATCCTGGGTCTGAAGCAGCAGATTGAAGA8240.1191478040076463No Hit
CAGACCCCAGGACCGGCAGTTCACTCATTGTCAAAACAGGGAGGGCAGGG8070.11668965756574096No Hit
CAGACCCCAGGACCAAGATAACCACTAGGAAGGTGAAAAGCATATGTAAA8030.11611127016764558No Hit
TCTCAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT8020.11596667331812176No Hit
GTTTCCCATCAGCCGCCCAAACACAGGACAATCTGAAACCTAGGAGACTC7990.11553288276955022No Hit
CAGACCCCAGGACCACACACATTCAAGACAACACCCCATACCTCATACCG7860.11365312372574027No Hit
CAGACCCCAGGACCTGTGCAATCAAATATTGTGGAGAATTCCCTAGCTGG7860.11365312372574027No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG7760.11220715523050184No Hit
GCTGAAAGGACTGGAGGACGAGCAAAAGATGCTTACCGAATCCGGAGATC7660.1107611867352634No Hit
TGGATACAGTGTCTCTCGACAGGCGAACAGGATATCTGAAGGGGTATACT7610.1100382024876442No Hit
CGGTTCTTTGCAGTCAGGGCGCAAAGGAACTGACATCATGTACACAGGCA7570.10945981508954884No Hit
CGATTCTCAGGGCTGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGC7520.10873683084192962No Hit
CAGACCCCAGGACCCATTAAGCCACCCCCGTGTTCCTGCCGTCAGTGCCA7400.1070016686476435No Hit
CGATTCTCAGGGCGCCCAGTGAACAAATCCGGGGGCAAGGCCAAAAAGAA7200.10410973165716667No Hit
CAGATGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGAT7190.10396513480764281No Hit
CCTGAATGCCCCAACAGCTCATTGAGAACGGGCCATGATGACAATGGCGG7090.10251916631240439No Hit
CGATTCTCAGGGCGCCAGCCGAGTGCTTATTTCTACAGATGTCTGGGCCA7090.10251916631240439No Hit
CCTGAATGCCCCAACAGCTCACTGAGAACGGGCCATGATGACAATGGCGG7070.1022299726133567No Hit
CGGTTCTTTGCAGTCAGGCCTGAACCCGTCCATTCAGAAAGCTTCAAATT6950.10049481041907059No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)