FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002271334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478010
Sequences flagged as poor quality0
Sequence length15-160
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC260345.44632957469509No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA199654.1766908642078615No Hit
GTTTCCCATCAGCCGCCCAAACCTTAGAAAGCTGGATGATACAGACCCTG150293.14407648375557No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA148463.1057927658417186No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA71111.4876257818874081No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG68661.436371624024602No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC57371.2001840965670174No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC52761.1037425995272065No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC50481.056044852618146No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG35580.744335892554549No Hit
CGGTTCTTTGCAGTCAGGCCTGACGAGTGCTGACAGCTATGAAGAAGACC35360.7397334783791134No Hit
GTTTCCCATCAGCCGCCCAAACCGTGGCAGCAGGACCGACAAGTCAAGTG33340.6974749482228405No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAGA33140.6932909353360809No Hit
CGATTCTCAGGGCGCTCTCCCAGCAAAGCAGTGGCCGCCCGGGCGGCGGG30720.6426643794062886No Hit
CGATTCTCAGGGCGCCATCGCCAAGTAAGAAAGTGAAGCAAATCAGAAAC30130.6303215413903475No Hit
GTTTCCCATCAGCCGCCCAAAGAACTGGTCAATGAGTGGTCATTGAAGAT29620.6196523085291104No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA27120.5673521474446142No Hit
CAGACCCCAGGACCGACGGGAAGGTTTTCCAGTTTCTTAATGCGAAATGC24850.5198636011798916No Hit
CGATTCTCAGGGCGCCCAGTGAAGAAATCTATACGAGATACTCCAGCCAA22980.48074308068868854No Hit
GGAACGATTTTCTGCTGAATTTCCCAACCCACCCCCAAACTAAACACTAA22880.47865107424530867No Hit
CGGTTCTTTGCAGTCAGGCCTGACTGGCATTGTATTAGCAAACTCATCAC21980.4598230162548901No Hit
GATAACTTCCAATCCAGGAGGCCGGCACGGAGGTGGTCAAGGCTAAAGCC20710.43325453442396605No Hit
CAGACCCCAGGACCGGCCGAACGCAGAGGTTGATTCTTCACCACACTGAA19890.41610008158825135No Hit
GTTTCCCATCAGCCGCCCAAACCAGGGCTATGAAGCAGGTAGCAGGTACC18840.3941340139327629No Hit
CCTGAATGCCCCAACAGCAGCAACCCTATCATCGTCTCCTGTGGCTGGGA18200.3807451726951319No Hit
CAGACCCCAGGACCGGACAGCCTGCGTTTGAATCTTGACTCTGCTGCATA17830.3730047488546265No Hit
CCTGAATGCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCAC17790.3721679462772745No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT17760.3715403443442606No Hit
CAGACCCCAGGACCTTCCATATTTTTATCTAAGGTAAAGTGCTTCTCAAT17680.3698667391895567No Hit
TCTCCACTCTGACGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC17110.3579423024622916No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA14520.3037593355787536No Hit
GCTGAAAGGACTGGAGGACGAGCAAAAGATGCTTACCGAATCCGGAGATC13750.28765088596472876No Hit
CGATTCTCAGGGCGCCCAACGATCTGTAAAGGTGTCTTCCACGCTATAGT12530.26212840735549464No Hit
GCTGAAAGGACTGGAGGGCCAGCAGAAGGTCAGCAACTTACTCTTAAAAC12010.2512499738499195No Hit
CAGACCCCAGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG12000.2510407732055815No Hit
GCTGAAAGGACTGGAGGGACTGTCCCTGGATCTGCGGAGGTAGACAGTTT11560.24183594485471016No Hit
CGATTCTCAGGGCGCCCAACGATAGCGACTCTGTCTCAGGGGATCTGCAT11380.2380703332566264No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG11130.23284031714817682No Hit
CGGTTCTTTGCAGTCAGGCCTCAAATAAATCATTATGAATCTTGTAGTTG10900.22802870232840317No Hit
TCTCACCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAG10820.2263550971736993No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG10780.22551829459634734No Hit
CAGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT10670.22321708750862954No Hit
NNNNNNNNNNNNNNN10590.22154348235392562No Hit
GATAACTTCCAATCCAGGAGGCCGCCAAGAAGAAGCCAGGCATCTTCATC10500.21966067655488378No Hit
CGATTCTCAGGGCTGCCCAACGATGACTAATCAAACTAACCTCAAAACAA10100.21129265078136442No Hit
CAGACCCCAGGACCCATTAAGCCACCCCCGTGTTCCTGCCGTCAGTGCCA9860.2062718353172528No Hit
CAGACCCCAGGACCAGCTGACAGCACTCACTGGGCGGATCCAGGAGGCCG9800.20501663145122487No Hit
CAGACCCCAGGACCAACCCGGAGCCCCCCTGGACCCCACACAGCAGTCCT9700.20292462500784503No Hit
CGATTCTCAGGGCGCCACGGTGTGCACCATTGTCTGGCCCACACTCACCT8710.18221376121838453No Hit
GCTGAAAGGACTGGAGGGACGGGGAGGATATCATCCTCAGGGTGCCCATC8640.18074935670801867No Hit
GATAACTTCCAATCCAGGAGGCAGTGGAGGAAGTGGGGGTTATGGTGGGA8410.17593774188824501No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG8410.17593774188824501No Hit
CCTGAATGCCCCAACAGCTCTCACGGCTGCTGCTCCTCTGGACTCCCCAA8320.17405493608920317No Hit
CGATTCTCAGGGCGCCCAACGATAGAAGATTCCTACAGAAAGCAAGTTGA8170.1709169264241334No Hit
CAGACCCCAGTCCACCATGATCCATCTGGGTCACATCCTCTTCCTGCTTT8060.16861571933641556No Hit
CGATTCTCAGGGCGCCCAGTGAACAAATCCGGGGGCAAGGCCAAAAAGAA8020.16777891675906362No Hit
GCAGAGAGGCTCAAAGGAGGGGAAGGAGCCCCGAGGAGGCTCTGAGTTGA7940.16610531160435976No Hit
CTGCAGAGAGGCCTAAGGCGGAGAAGAACGCTGTCTCCCATCGCTTCCGG7750.16213049936193805No Hit
CAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC7620.15941089098554423No Hit
CCTGAATGCCCCAACAGCCCTCCTGTGCCTGCTTCCCTGTGTGTCGGGGT7510.15710968389782642No Hit
CAGACCCCAGGACCAGACAGATTCACAGCCGAATTCTACCAGATGTACAA7430.15543607874312254No Hit
CGATTCTCAGGGCGCCAGTCAGCCTGGTTGTGCGAATGGAGGTCGTGTTC7030.14706805296960315No Hit
CAGATGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGAT6850.14330244137151943No Hit
CGATTCTCAGGGCTGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGC6750.1412104349281396No Hit
CGATTCTCAGGGCGCCCACGATGCTCTTAGCTGAGTGTCCCGCGGGGCCC6580.13765402397439383No Hit
CGATTCTCAGGGCGCCCAACGATAAAGAAAAGCACATATTTCAGTGATGA6540.13681722139704192No Hit
TCTCAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT6510.13618961946402794No Hit
CGATTCTCAGGGCGCCAGCCGAGTGCTTATTTCTACAGATGTCTGGGCCA6280.1313780046442543No Hit
CGATTCTCAGGGCGCCCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCA6220.13012280077822638No Hit
CTCAGCAGAGATGCCTAAGGCGGAGAAGAACGCTGTCTCCCATCGCTTCC6180.12928599820087447No Hit
CGGTTCTTTGCAGTCAGGCCTGTTATAACCGCTGAGCAGCCACACTCGCA6170.12907679755653648No Hit
CGGTTCTTTGCAGTCAGGGCGCAAAGGAACTGACATCATGTACACAGGCA6120.12803079433484657No Hit
GTTGTCTCCAGATCCAAGACAGAGAATTTCCCCCTCACTCTGGAGTCAGC6080.1271939917574946No Hit
CGATTCTCAGGGCGCCCAGTGACACCATCGAAAATGTGAAGGCCAAGATC6060.12677559046881864No Hit
GTTTCCCATCAGCCGCCCAAACCCACCTTCAGGTCCTACCTCCTGCCACC5710.11945356791698918No Hit
CGATTCTCAGGGCGCAAAGGAACTGACATCATGTACACAGGCACGCTTGA5670.11861676533963725No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG5660.11840756469529926No Hit
GCTGAAAGGACTGGAGGGACAAGGATCTTAAACCCAAAGTACGAGAAATG5610.11736156147360935No Hit
TCTCACCTAAATCTCCAGACAACCTTAGCCAAACCATTTACCCAAATAAA5550.11610635760758144No Hit
CTCAGCAGAGATGATAACATGTTTCAGATTGGGAAAATGAGGTACGTTAG5490.11485115374155352No Hit
GCTGAAAGGACTGGAGGGACGCCACTGCTGTACGCTGTGCGCGGGAACCA5420.11338674923118763No Hit
CAGACCCCAGGACCGAGCAGGATGGGCATGATATTGAGAACGAGTGTCTA5360.11213154536515973No Hit
TCTCACCTAAATCTCCAGACAAAGCTAAGACCCCCGAAACCAGACGAGCT5300.11087634149913182No Hit
CTCAGCAGAGATGCCTGATGGTTCAAGTAGAGGCCCAGTACATCTTGATC5250.10983033827744189No Hit
CGATTCTCAGGGCGCCAAGTAGACAAAAATCTTTGCCCACAGTTACATTC5230.10941193698876593No Hit
CGATTCTCAGGGCGCCAGGCTGTCCTTAGCATGGTCCGGAAGGCAAAGTA5130.10731993054538608No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG5100.10669232861237213No Hit
TGGATACAGTGTCTCTCGACAGGCGAACAGGATATCTGAAGGGGTATACT5090.10648312796803414No Hit
CAGACCCCAGGACCGGCCGAACGCAGAGAAGGTAGAAGATAGCACCATGC5030.10522792410200624No Hit
CAGACCCCAGGACCTCAGAGTGTGACTGTATTTGGAGATAGGATCTCTCA4860.1016715131482605No Hit
CGATTCTCAGGGCGCCACAGGAACATGCGACTTGGATGTTCCTGAAATTG4840.10125311185958452No Hit
CGATTCTCAGGGCGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAAC4830.10104391121524654No Hit
CGATTCTCAGGGCGCCCAGTGACACCATTGAGAATGTCAAAGCCAAAATT4810.10062550992657056No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC4790.10020710863789462No Hit
GCTGAAAGGACTGGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA4790.10020710863789462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)