FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478010
Sequences flagged as poor quality0
Sequence length15
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN4795110.031380096650699No Hit
TCGCCAAGGTTGTTT169913.5545281479466957No Hit
ACTGCAGCCTCTTCG86021.799543942595343No Hit
GTAATCCGTGTTGAT59591.24662663961005No Hit
TAATGCCGTGTATCA33070.691826530825715No Hit
GAATAGCAGATCTAG31630.6617016380410451No Hit
CAGTCAATTTCAGGT28650.5993598460283258No Hit
GCAGTAGGTCAAGGG21880.4577310098115102No Hit
AGCAGGTTGAGTTGG19830.41484487772222334No Hit
TGACGTTAGTGACCT17670.3696575385452187No Hit
TACCGGAGTGGGGGG16290.3407878496265769No Hit
AAGGCTAATAGGGTC14430.30187652977971174No Hit
TCTACAGTTGAGGTT11970.2504131712725675No Hit
TCTTAGGGACGTTTT11420.23890713583397835No Hit
GGATTCAATCATCGT10840.22677349846237527No Hit
AGGACATGTTTATGC10640.22258948557561556No Hit
TTTTTTTTTTTTTTT10050.2102466475596745No Hit
TACCGGAGTCCTCGC9640.2016694211418171No Hit
TATCGCGACCTGTAA9200.1924645927909458No Hit
ATAGCGACGCAAACC8710.18221376121838453No Hit
CGTATGACTTAGTTA8590.17970335348632874No Hit
AGTTCGCTGGTGATA8510.17802974833162488No Hit
CGCCATTTCTGGTGT7580.1585740884081923No Hit
ACGGATAAATTGTTT7420.15522687809878455No Hit
GTAACTGTGGTTATG7220.15104286521202484No Hit
CGAGTAGTCTGGCTT7130.149160059412983No Hit
TACCGGACTTCATAT6810.1424656387941675No Hit
CGTAACTGGTCGGCT6400.1338884123763101No Hit
GATCACAACTGGGCA6290.1315872052885923No Hit
TAGACGCGGTCCTCC6260.13095960335557832No Hit
TAGAGCCGCACCCGT6100.12761239304617059No Hit
GCAGTAGTATGTGTG6050.12656638982448065No Hit
AGGACATTTTGCGGT5950.12447438338110081No Hit
CTGATGGTTTAGGGG5910.12363758080374887No Hit
AGATCACTAAGGCTG5690.1190351666283132No Hit
CGCCATTTTTGCATT5670.11861676533963725No Hit
CAGTCAATTTCAGGG5670.11861676533963725No Hit
TGATGACTATAATTC5650.11819836405096128No Hit
ACTCGGTAGGTCGGG5540.11589715696324344No Hit
CGTCAGAGGCAAACG5300.11087634149913182No Hit
TGAGCAGCGTATGGG5220.10920273634442794No Hit
CGTTGAAAACCTTCC5200.10878433505575197No Hit
TAACAGCTTTGATAG5140.10752913118972406No Hit
ATTCCAGAGTAATTT5060.1058555260350202No Hit
TATCGCGGGCCATTT4970.10397272023597832No Hit
TCGCCAAGGGTGTTT4810.10062550992657056No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)