FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277621
Sequences flagged as poor quality0
Sequence length15
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN2852010.272998080116418No Hit
ATAACCGTGCGTGTT24560.8846593017098849No Hit
CAGCACTACTCGAAC23760.8558430378105404No Hit
GGAATCGCTCATTTC16880.6080231682761751No Hit
TACCAGTCAATCGGT15140.5453477942951002No Hit
TCGACTCAAGGTATG11510.41459399685182313No Hit
TACCATGAATGTTAT9850.35480024926068277No Hit
AGATACCTAGAGGGG9800.3529992327669737No Hit
GCTTCATCCGGCACT9640.34723597998710476No Hit
GGAATCGGTGAAGAT9250.33318805133617413No Hit
CGATATCCGGCACGT8650.31157585341166555No Hit
GGAATCGTGGTCTTC8650.31157585341166555No Hit
CGAGATTGGCAGCGG8080.2910442653833824No Hit
CAGTTCAAGTTACGG7680.27663613343370996No Hit
AGTCTCCGGCGGCTT7310.263308611380263No Hit
TCGCCAAGCGAGTAG6960.2507014959242997No Hit
TCGCCAATGCATTTG6570.23665356727336906No Hit
TGGCTATGGGAAAGT6150.2215250287262131No Hit
GCTTCATGCTCGACT5990.2157617759463441No Hit
CGAATTCGTGAAGAT5380.1937893747230937No Hit
AGTACCTGCATACCT5020.18082205596838855No Hit
CCGCATTGCGATACG4850.1746985998897778No Hit
TGGCTATGTCATGCC4770.17181697349984332No Hit
GAATAGCAAGGATGT4770.17181697349984332No Hit
GAATAGCTCGCACGC4760.1714567702011015No Hit
AGAGCATCTCACGGG4700.16929555040865063No Hit
AACGGTGGGCGTCTG4570.16461290752500712No Hit
CACCTAGTTATGTTT4480.1613710778363308No Hit
ATGCGCAAAGTTAAG4270.1538068085627528No Hit
AGATACCGGATCCTT4130.1487639623803675No Hit
TGGATGCTTAAATGT4100.14768335248414205No Hit
GGACTAAATCGGTTA3930.1415598964055313No Hit
AGTACCTACATACAC3650.1314742040407606No Hit
CGACACTTTTTCATT3640.1311140007420188No Hit
TCAACGTCTTGTTGA3600.12967318754705154No Hit
CGCTACAGCTATGTT3590.12931298424830975No Hit
ACGTCAATAGGCAAC3570.12859257765082613No Hit
ATTCGCGTCTTTCTT3570.12859257765082613No Hit
GGTATCACGATACTT3560.12823237435208432No Hit
CGAGATTGTTTGGTT3530.12715176445585888No Hit
CTAAGCGGTCGGCTT3470.12499054466340803No Hit
CGATATCGTTTCTAC3450.12427013806592441No Hit
TCGCCAAGAGCGGAC3300.11886708858479726No Hit
GCAATTGTTGCGGAC3220.11598546219486279No Hit
GTCAGTGAGAGAGAG3160.11382424240241193No Hit
GCGACTAAAGACTGT3140.11310383580492829No Hit
TAATGCCACTCTGTC3080.11094261601247744No Hit
CGCCATTCATTCTCT3020.10878139622002658No Hit
GAAGTCCAAGAAATT2970.10698037972631755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)