FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343721
Sequences flagged as poor quality0
Sequence length15
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN341959.948475653218743No Hit
CTTGTACACTGGCTT29770.8661094317775172No Hit
GGATCTATGATTGGG26880.7820296112253834No Hit
CGGTCTAGGCTTTTT26230.7631189249420315No Hit
TATCGCGGTCCTAAC23480.6831121752816965No Hit
CTACAAGAAGTTATT19430.5652840530546577No Hit
GTATGCGTACGATAG15090.4390188554088926No Hit
CAACTGATCGCACGG14490.4215628373011832No Hit
CAACTGATGAGAAGC14420.4195263018552837No Hit
TCGACTCTGGACAGA14240.4142894964229709No Hit
ACGATGCAGTAGGGG12930.37617719022113866No Hit
CGAATTCACGCTTGC11790.3430107558164907No Hit
TCAACGTCTAAGGCT11490.334282746762636No Hit
CTAGAGTCGCAGCTT11060.32177260045211087No Hit
ACGTGGTGGTTATCT11030.32089979954672543No Hit
CGGAATGTCCTACAT10780.31362645866851313No Hit
TACGAGGATAATGCA10210.29704324146618916No Hit
TACCGATGAAAAAGG9830.2859877633313065No Hit
CATCCGTAGTAGGAG9370.2726048161153959No Hit
CGGTATTTGGGACGG9010.2621312052507702No Hit
GCATGGATCATTGCG8920.25951280253461384No Hit
ACGCTTCCACGATAC8420.2449661207781893No Hit
ACGTTGGACATTATT8360.24322051896741834No Hit
AGGCTAGGCGTCCTT8340.24263865169716134No Hit
AAGGTACAGCGCGTT7840.22809196994073683No Hit
ATGAGCGGTATGATG7370.21441808908969776No Hit
TAGACGCGGCTTATT7300.21238155364379832No Hit
CTATTCGGTAGGCGG6220.1809607210499213No Hit
AGTTGCAGAATGATT6170.17950605287427884No Hit
CTAGAGTGCTTGTTC6030.17543298198247997No Hit
TCAGTGGATCCGTAG5870.17077804382042414No Hit
AATCACGCAGATGTG5620.16350470294221187No Hit
GGTTCAAACGTCCGT5470.15914069841528447No Hit
AGTCTCCTGGGTTGT5400.15710416296938504No Hit
GCCTAATTAATGCCT5370.1562313620639996No Hit
CCATGACTTAAACGC5230.15215829117220073No Hit
GTTGCTAGTATCGTT5200.15128549026681523No Hit
TCGAAGGAATCTTAG4830.1405209457670611No Hit
TAGACGCAGTTGCGG4800.13964814486167562No Hit
CGGTATTAAGTAGGC4590.1335385385239773No Hit
GCTTAAGTCTGTTAG4420.12859266672679295No Hit
TCGCCAAGCGGTTAG3990.11608252041626783No Hit
TGCACTCAAATGGAT3870.11259131679472595No Hit
CTTCGAAGGTTTCGA3820.11113664861908348No Hit
GAATAGCCTCGACGT3810.110845714983955No Hit
AAGCCTTTGGAGAAG3720.1082273122677986No Hit
CGGATGTCTGAAGGG3690.10735451136241311No Hit
AACGTTCAGATGTAT3500.10182677229497179No Hit
TCTGGAGCGGTGGGT3440.10008117048420084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)