FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002271342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241279
Sequences flagged as poor quality0
Sequence length15-160
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC154226.391770522921597No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA88923.685360101790873No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA72252.994458697192876No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG59842.480116379792688No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA51282.125340373592397No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGG40241.6677787955023022No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC34571.4327811371897263No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC27241.1289834589831689No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG26681.1057738137177293No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG25461.0552099436751643No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC23930.9917978771463741No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA22350.926313520861741No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC21100.8745062769656704No Hit
GTTTCCCATCAGCCGCCCAAACCTTAGAAAGCTGGATGATACAGACCCTG20380.8446653044815339No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCA20000.8289159023371283No Hit
GTTTCCCATCAGCCGCCCAAACCGTGGCAGCAGGACCGACAAGTCAAGTG18090.7497544336639326No Hit
CGATTCTCAGGGCGCCATCGCCAAGTAAGAAAGTGAAGCAAATCAGAAAC15400.6382652447995889No Hit
GATAACTTCCAATCCAGGAGGCCGGCACGGAGGTGGTCAAGGCTAAAGCC15320.6349495811902404No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG15170.628732711922712No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG13870.5748531782707985No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA11660.48325797106254587No Hit
GATAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACAT11360.4708242325274889No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG11310.46875194277164617No Hit
GCAGAGAGGCTCAAAGGAGTCAACATTTAGATCTGGAAGGGACAAGTCAT11050.4579760360412634No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC10860.4501013349690607No Hit
CCTGAATGCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCAC9690.40160975468233867No Hit
GCTAAGTGCCTCCCAAATTCACCCTGTAGCCTTGAGATCCAGGCTACGAA9280.38461697868442757No Hit
CGATTCTCAGGGCGCCCAGTGAAGAAATCTATACGAGATACTCCAGCCAA8940.3705254083446964No Hit
CAGACCCCAGGACCCCCTCTCAGGTCTAGAAGATCCAGTTGGGCAGTGTT8600.3564338380049652No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC8290.3435856415187397No Hit
CGATTCTCAGGGCGCTCTCCCAGCAAAGCAGTGGCCGCCCGGGCGGCGGG8120.3365398563488741No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATC7980.33073744503251423No Hit
CCTGAATGCCCCAACAGCAGCAACCCTATCATCGTCTCCTGTGGCTGGGA7740.3207904542044687No Hit
CAGACCCCAGGACCAGCTGACAGCACTCACTGGGCGGATCCAGGAGGCCG7730.3203759962533001No Hit
CAGACCCCAGGACCCATTAAGCCACCCCCGTGTTCCTGCCGTCAGTGCCA7720.31996153830213153No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA7320.303383220255389No Hit
GATAACTTCCAATCCAGGAGGCAGTGGAGGAAGTGGGGGTTATGGTGGGA7160.29675189303669197No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT6810.2822458647457922No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTGTATC6490.26898321030839817No Hit
CGGTTCTTTGCAGTCAGGCCTGACTGGCATTGTATTAGCAAACTCATCAC6400.26525308874788106No Hit
GTTTCCCATCAGCCGCCCAAACCAGGGCTATGAAGCAGGTAGCAGGTACC6380.2644241728455439No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC6260.25945067743152117No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATTCAGCCTGCAGA6110.2532338081639927No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA6060.25116151840814993No Hit
CGATTCTCAGGGCGCCACCCTTGACGCTTGCAGCTTCGGAGTCACGGGTT5870.24328681733594718No Hit
TCTCCACTCTGACGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC5540.22960970494738456No Hit
CAGACCCCAGGACCGGCCGAACGCAGAGGTTGATTCTTCACCACACTGAA5440.22546512543569894No Hit
TCTCACCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAG5380.22297837772868753No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAGA5320.22049163002167615No Hit
CGATTCTCAGGGCGCCCAACGATCTGTAAAGGTGTCTTCCACGCTATAGT4960.20557114377960783No Hit
CAGACCCCAGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG4560.18899282573286527No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG4330.1794602928559883No Hit
CCTGAATGCCCCAACAGCCCTCCTGTGCCTGCTTCCCTGTGTGTCGGGGT4030.16702655432093136No Hit
CCTGAATGCCCCAACAGCATTCTTTTTCTCCATAGCACATAACACATTTG3960.1641253486627514No Hit
CAGACCCCAGGACCGGCGGCACAATTTGCAGGGCCAAGTGCAAAACGAAC3940.1632964327604143No Hit
CGGTTCTTTGCAGTCAGGCCTGTTATAACCGCTGAGCAGCCACACTCGCA3850.15956631119989723No Hit
CAGACCCCAGGACCGGCTTCTGGGCGGCTGAAAGGGGCAAGGAGGCAAGG3840.15915185324872866No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG3660.1516916101276945No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG3620.15003377832302023No Hit
CAGACCCCAGGACCGACGGGAAGGTTTTCCAGTTTCTTAATGCGAAATGC3560.14754703061600885No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA3550.14713257266484028No Hit
CAGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT3520.1458891988113346No Hit
CCTGAATGCCCCAACAGCTCTCACGGCTGCTGCTCCTCTGGACTCCCCAA3470.14381690905549177No Hit
NNNNNNNNNNNNNNN3390.14050124544614326No Hit
CGATTCTCAGGGCGCCCAGTGACACCATCGAAAATGTGAAGGCCAAGATC3330.13801449773913188No Hit
CGATTCTCAGGGCGCCCAACGATAAAGAAAAGCACATATTTCAGTGATGA3310.13718558183679475No Hit
CGATTCTCAGGGCTGCCCAACGATGACTAATCAAACTAACCTCAAAACAA3280.13594220798328907No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC3260.13511329208095194No Hit
CGATTCTCAGGGCGCCCACGATGCTCTTAGCTGAGTGTCCCGCGGGGCCC3210.13304100232510913No Hit
CGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCA3200.13262654437394053No Hit
CAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC3090.12806750691108634No Hit
CGATTCTCAGGGCTGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGC3080.12765304895991778No Hit
CAGACCCCAGTCCACCATGATCCATCTGGGTCACATCCTCTTCCTGCTTT3060.12682413305758064No Hit
GCTGAAAGGACTGGAGGGACTGTCCCTGGATCTGCGGAGGTAGACAGTTT2980.12350846944823213No Hit
CAGACCCCAGGACCACTCCGCCCCCGCTAACTCAATGCAGCTGACCCTTA2740.11356147862018659No Hit
CTCAGCAGAGATGCCTGAATCTGCAATCTCGATTTGGGTGGCTGCAGCTG2720.11273256271784947No Hit
CGGTTCTTTGCAGTCAGGCCTGACGAGTGCTGACAGCTATGAAGAAGACC2630.10900244115733237No Hit
CGATTCTCAGGGCGCCCAACGATCTATAAACCTAGCCATGGCCATCCCCT2460.10195665598746678No Hit
TCTCACCTAAATCTCCAGACAAAGCATCAAACAGCAGGGAGCTAGTGGAG2450.10154219803629823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)