FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241279
Sequences flagged as poor quality0
Sequence length15
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN179137.42418527928249No Hit
GATGTACTGTCGTGG27491.139344907762383No Hit
GCCTACAGCGGCAGC19670.8152387899485658No Hit
GCTGACAGGAGGAAG14880.6167134313388235No Hit
CGGTATTTGTACATC13670.5665640192474273No Hit
CAGTCAATTATTTGG13190.5466700375913361No Hit
GCTGACAACGCCGCT12700.5263615979840766No Hit
CGCATATGCTTGCCG11550.4786989335996916No Hit
AGGCATGTCAGAAGG10640.4409832600433523No Hit
CCTGACTTGCTATCT10570.4380820543851724No Hit
CGCATATTTGGGTAA8400.3481446789815939No Hit
ATGTCCGATCCTGGT8200.3398555199582226No Hit
GACGTAGCCTGATCG7810.32369165986264864No Hit
CTAGAGTAGGGAGTT7540.3125012951810974No Hit
GCCATAGACGCGGCG7480.310014547474086No Hit
GGACTAATTTATACG7240.30006755664604046No Hit
AGGCATGAGCGTTCT7210.29882418279253475No Hit
CAGTTGTGTATATAA6830.28307478064812935No Hit
CTACAGCTTTTGGGT6700.27768682728293803No Hit
CTACAAGTCACTTTG6670.27644345342943233No Hit
TCAGTTGTAAGCAGT6660.27602899547826376No Hit
GCTGACATTCGTACT6320.2619374251385326No Hit
TCCTGTATGCCTAAT6170.2557205558710041No Hit
CGCATATTAAGATTT6050.25074706045698136No Hit
CTAGAGTGCTAATTT5960.24701693889646426No Hit
GACGTTGGCCCTCCT5920.24535910709179No Hit
ACTGCAGGGTATCAC5900.24453019118945285No Hit
TAATGCCATCCAAGT5750.23831332192192442No Hit
TACCAGTTACGGGGG5710.23665549011725015No Hit
ATAACCGTATAGGCG5700.23624103216608158No Hit
TGACACAGCGTATGT5500.22795187314271032No Hit
TACCAGTGGGATTAC5480.22712295724037318No Hit
CTAGAGTTGTGTCAG5420.22463620953336177No Hit
AAGGTACATTGGTCT5260.21800488231466475No Hit
GGAGACTTGTTTAGT5250.21759042436349618No Hit
CTGATCAAGGTTTAA5140.21303138690064197No Hit
CGCATATTTAGGCAT5060.20971572329129348No Hit
CGATATCGGTTGGAT4710.19520969500039376No Hit
CGGTTATCTCGGTAG4550.1885783677816967No Hit
GATAGTCAACGGAAT4520.187334993928191No Hit
CTACAGCCGAGTCTT4380.1815325826118311No Hit
AGATGCGAGGCCGAT4320.17904583490481973No Hit
TGAAGCTCGTTTATT4290.17780246105131406No Hit
CAGTTGTTGCCTGCC4280.17738800310014546No Hit
CAGTTGTGGGGGTAC4270.17697354514897692No Hit
CGAGTGAGCGGCTAA4210.17448679744196552No Hit
TCCGCATCCTTATGG4090.16951330202794276No Hit
GTCACTAGGAATTTT3970.16453980661391998No Hit
ACCGATCTAGATTTT3960.1641253486627514No Hit
TAGAGCCTCCCAAGT3930.1628819748092457No Hit
GGACTAAGTCGAGAG3870.16039522710223436No Hit
TCTGGAGGCATTTGA3410.1413301613484804No Hit
TGACACACGGCTGTG3390.14050124544614326No Hit
GCAACTAGCGTGTAA3370.13967232954380612No Hit
ATGCCAAGTTTTGAA2870.11894943198537793No Hit
AAGGTACGGGGCGTC2840.11770605813187222No Hit
CAGCACTGGGAGACG2820.11687714222953512No Hit
TTGCAGATGCGTGTG2800.11604822632719797No Hit
ACTCGGTTTTTGATT2770.11480485247369228No Hit
ACTGCAGTCGAACTT2710.11231810476668089No Hit
TGCACAACGGGCTGC2680.11107473091317521No Hit
TGACACATTTGTCGT2560.10610123549915243No Hit
GATGTACTGTCGCGG2510.10402894574330962No Hit
CGGTTATCGGGATAA2500.10361448779214104No Hit
CTAGAGTGCGTAAGT2430.1007132821339611No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)