FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002271344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500832
Sequences flagged as poor quality0
Sequence length15-160
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC283335.65718644176091No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA222594.444404510893873No Hit
CCTGAATGCCCCAACAGCTCAGTGCTTTCCTGACACTCCAGTGTCTGGGG100912.0148472941026134No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC94941.8956456456456456No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA72821.4539805763210019No Hit
CTGCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGG50491.008122484186314No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC47370.9458261452942305No Hit
CGATTCTCAGGGCGCCCAGTGAAGAAATCTATACGAGATACTCCAGCCAA33840.6756756756756757No Hit
GATAACTTCCAATCCAGGAGGCCGGCACGGAGGTGGTCAAGGCTAAAGCC32370.6463245160053671No Hit
GTTTCCCATCAGCCGCCCAAACCGTGGCAGCAGGACCGACAAGTCAAGTG31790.634743786339531No Hit
CCTGAATGCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCAC30980.6185706983579324No Hit
CAGATGGCTATAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACT28110.5612660532873298No Hit
CAGACCCCAGGACCGCCCTTAGAAGGCTGCGCCGGCCCGCGAGAGGTCTG27960.5582710369944412No Hit
CGGTTCTTTGCAGTCAGGCCTGTTATAACCGCTGAGCAGCCACACTCGCA25570.5105504440610823No Hit
GGAACGATTTTCTGCTGAATTTCCCAACCCACCCCCAAACTAAACACTAA25370.5065570890038975No Hit
CGATTCTCAGGGCGCTCTCCCAGCAAAGCAGTGGCCGCCCGGGCGGCGGG24690.49297968180946905No Hit
CAGATGGCTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACT24490.4889863267522842No Hit
CGGTTCTTTGCAGTCAGGCCTGACTGGCATTGTATTAGCAAACTCATCAC23550.4702175579835154No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG22970.4586368283176794No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA22870.45664015078908693No Hit
GATAACTTCCAATCCAGGAGGCAGTGGAGGAAGTGGGGGTTATGGTGGGA22740.4540444700019168No Hit
GTTTCCCATCAGCCGCCCAAACCAGGGCTATGAAGCAGGTAGCAGGTACC21110.4214986262858603No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG19610.39154846335697396No Hit
TCTCACCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAG18370.36678966200242796No Hit
CTGCAGAGAGGCCTAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGG18140.3621973036866654No Hit
CCTGAATGCCCCAACAGCAGCGCGGGGCCGCCAAGGAGGCCGCGGGGAAG17800.3554086000894512No Hit
CGATTCTCAGGGCGCCCAACGATCTGTAAAGGTGTCTTCCACGCTATAGT16500.3294517922177497No Hit
CAGACCCCAGGACCAGCTGACAGCACTCACTGGGCGGATCCAGGAGGCCG16290.3252587694077056No Hit
CCTGAATGCCCCAACAGCAGCAACCCTATCATCGTCTCCTGTGGCTGGGA16020.319867740080506No Hit
CGATTCTCAGGGCGCCATCGCCAAGTAAGAAAGTGAAGCAAATCAGAAAC14930.2981039550188486No Hit
NNNNNNNNNNNNNNN14510.28971790939876046No Hit
CAGACCCCAGGACCGACGGGAAGGTTTTCCAGTTTCTTAATGCGAAATGC14490.289318573893042No Hit
CGATTCTCAGGGCGCCAGTTCTCACTGTTCTGCAATGCTCATGGCAAGTT14450.288519902881605No Hit
CAGACCCCAGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG14030.28013385726151685No Hit
TCTCACCTAAATCTCCAGACAAAGCATCAAACAGCAGGGAGCTAGTGGAG13810.27574116669861354No Hit
CGATTCTCAGGGCTGCCCAACGATGACTAATCAAACTAACCTCAAAACAA12990.25936841096415564No Hit
CAGACCCCAGGACCCATTAAGCCACCCCCGTGTTCCTGCCGTCAGTGCCA12820.25597405916554855No Hit
GCTGAAAGGACTGGAGGACGAGCAAAAGATGCTTACCGAATCCGGAGATC12710.25377771388409687No Hit
CGGTTCTTTGCAGTCAGGCCTGACGAGTGCTGACAGCTATGAAGAAGACC12510.249784358826912No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA11390.22742157050667688No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG11380.22722190275381765No Hit
CAGACCCCAGGACCAAGACCTGCACACAGCCAGGACTCAAGAAGTGTTCG10080.2012650948821162No Hit
CGATTCTCAGGGCGCCCAACGATAAAGAAAAGCACATATTTCAGTGATGA9650.19267938150916875No Hit
GCTGAAAGGACTGGAGGGCCAGCAGAAGGTCAGCAACTTACTCTTAAAAC9580.19128170723915405No Hit
CGGTTCTTTGCAGTCAGAGAAAAAAATGTATTCACTAAACTAATAGAAAA9450.1886860264519839No Hit
CGGTTCTTTGCAGTCAGGCCTCAAATAAATCATTATGAATCTTGTAGTTG9370.18708868442910997No Hit
CGATTCTCAGGGCGCCAGTGACCTCAGGAGTTTGAGACCAGCCTGGCCAA9290.18549134240623602No Hit
CAGACCCCAGGACCGGCTTCTGGGCGGCTGAAAGGGGCAAGGAGGCAAGG9180.18329499712478436No Hit
CAGACCCCAGGACCACTCCGCCCCCGCTAACTCAATGCAGCTGACCCTTA9090.18149798734905118No Hit
TCTCACCTAAATCTCCAGACAACCTTAGCCAAACCATTTACCCAAATAAA9010.17990064532617725No Hit
GCAGAGAGGCTCAAAGGAGTCAACATTTAGATCTGGAAGGGACAAGTCAT8990.17950130982045875No Hit
CTGCAGAGAGGCCGAGCGCTGCATGTGAAAGATAATGGGGGAATTTATAT8690.1735112772346815No Hit
CGATTCTCAGGGCTGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGC8650.17271260622324452No Hit
CCTGAATGCCCCAACAGCTCATTGAGAACGGGCCATGATGACAATGGCGG8570.1711152642003706No Hit
CGATTCTCAGGGCGAGGGTTATCCATTGTGTTCCGGATGTGCTGACCTCT8560.17091559644751134No Hit
GTTTCCCATCAGCCGCCCAAACCTCGCAGGGCTCCAGCAGGCCAACCGGC8490.16951792217749664No Hit
GTCTCTAGATTAAACACAGAGGACCTCCCCCTCACTCTGGAGTCTGCTGC8190.16352788959171938No Hit
CAGACCCCAGGACCTTCCATATTTTTATCTAAGGTAAAGTGCTTCTCAAT8120.16213021532170468No Hit
GGGGTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGG8120.16213021532170468No Hit
CGATTCTCAGGGCGCCCAACGATCTATAAACCTAGCCATGGCCATCCCCT7900.15773752475880135No Hit
CGGTTCTTTGCAGTCAGGCTTCATACGCTATTGTCCTGCCCGTTAGAGCA7560.15094882116158712No Hit
CGATTCTCAGGGCGCCCAACGATGTAAAATTTATAATGACCAGCATCCAA7510.1499504823972909No Hit
CGATTCTCAGGGCGCAAAGGAACTGACATCATGTACACAGGCACGCTTGA7460.1489521436329947No Hit
CCTGAATGCCCCAACAGCTCCGAAGAGACAGAGACCATGGAGAACGGGCC7300.1457574595872468No Hit
TCTCACCTAAATCTCCAGACAAAGCTGGGAGGCCCAGCAAAAAGAAAAGC6800.13577407194428473No Hit
CAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC6570.13118171362852216No Hit
CGATTCTCAGGGCGCCCAGTGACACCATCGAAAATGTGAAGGCCAAGATC6530.13038304261708517No Hit
CTCAGCAGAGATGATAACATGTTTCAGATTGGGAAAATGAGGTACGTTAG6420.1281866973356335No Hit
GTTTCCCATCAGCCGCCCAAACCGAAGTCATAGCCACACTCAAGAATGGG6310.12599035205418183No Hit
CCCTGATGGCTACAATGTCTCTAGATCAAACACAGAGGACCTCCCCCTCA6280.12539134879560412No Hit
GCTGAAAGGACTGGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA6260.12499201328988563No Hit
GTCTCCAGATCAACCACAGAGCTGCAGGCAGCCGAGGTTTGATCCATAGC6240.12459267778416716No Hit
CGATTCTCAGGGCGCCACCCTTGACGCTTGCAGCTTCGGAGTCACGGGTT6200.12379400677273017No Hit
CAGACCCCAGGACCGGCGCAAGGAGGTCACGGATGAGATAGTGAAAGAGT6180.1233946712670117No Hit
GATAACTTCCAATCCAGGAGGCAGAGGTTGCAGTGAGCCAAGATCGCACC6170.12319500351415245No Hit
CCCTGATGGCTACAATGTCTGAATCTTTGGAAAAGAAAGCCCCTTTCTCA6120.12219666474985624No Hit
GTTTCCCATCAGCCGCCCAAACCTGGAGACCTGATTGAGATTTTCCGCCT6080.12139799373841927No Hit
CAGACCCCAGGACCGGCACTTCTTAATGTTTGTGGCTTTCTCTCCAGCCT6080.12139799373841927No Hit
TCTCAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT6040.1205993227269823No Hit
TCTCACCTAAATCTCCAGACAAAGCTAAGACCCCCGAAACCAGACGAGCT6020.12019998722126381No Hit
CGATTCTCAGGGCGCCAGCCGAGTGCTTATTTCTACAGATGTCTGGGCCA5910.11800364193981215No Hit
CCTGAATGCCCCAACAGCCCTCCTGTGCCTGCTTCCCTGTGTGTCGGGGT5870.11720497092837519No Hit
CGATTCTCAGGGCGCCCTGGCTTCTCCCCAAGGAGATGAGGAGCGGTGAT5850.11680563542265669No Hit
CAGACCCCAGTCCACCATGATCCATCTGGGTCACATCCTCTTCCTGCTTT5720.11420995463548655No Hit
CGATTCTCAGGGCGCCCAACGATACCAAAGAATATCCCAATTATCCATAA5630.11241294485975337No Hit
CAGACCCCAGGACCTATGCCAGTCTGGACCAGCTGTCGGAGTTAAAGAAT5630.11241294485975337No Hit
CAGACCCCAGGACCGGCCACTGCCCCAGGCACCCTGTGTGACTTCCCTGA5550.11081560283687944No Hit
GTCTCCAGATCAACCACAGAGGATGCTGACATCATTTGTATTATGTTCCA5390.10762091879113155No Hit
CAGATGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGAT5340.10662258002683533No Hit
TCTCACCTAAATCTCCAGACAAAGCAGCATCTGGAAGCTTCCATGGTGAA5290.10562424126253914No Hit
CAGACCCCAGGACCTCCCTGACCCCCAACCAGGCCAGCGGAAGCCTGCCA5280.10542457350967989No Hit
CGATTCTCAGGGCGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAAC5190.10362756373394671No Hit
CGATTCTCAGGGCGCCCAACGATAGCGACTCTGTCTCAGGGGATCTGCAT5160.10302856047536897No Hit
CGGTTCTTTGCAGTCAGGCCTGAACCCGTCCATTCAGAAAGCTTCAAATT5100.10183055395821354No Hit
CCTGAATGCCCCAACAGCTGACCAAAGAAAGCCCAAGTTCTCCTTCTGTC5100.10183055395821354No Hit
CGATTCTCAGGGCGCCAGCCTGGCAGTCTCCACGGTGACTCTGCTGCTTC5090.10163088620535429No Hit
CGATTCTCAGGGCGCCCACGATGCTCTTAGCTGAGTGTCCCGCGGGGCCC5050.10083221519391732No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)