FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500832
Sequences flagged as poor quality0
Sequence length15
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN5143510.269910868315124No Hit
AGTGAGCTTTAAGGG102912.054780844674462No Hit
CACCTAGACGGTGGC78501.5673918599450516No Hit
CGAGTGATAGACTTG35620.7112165356846208No Hit
ACGGTAGCAGCAGCC30230.6035956168934893No Hit
TATAGGCGTAAATCT28990.5788368155389432No Hit
CACCTAGGGGGTGAC25480.5087534342853491No Hit
ACTTGGCTTGTTTTC14000.2795348540029391No Hit
AACTCGTGTGCGCGG12890.25737173343556324No Hit
GGCGATTCCCCGGTG12520.24998402657977126No Hit
CACCTAGACGGAGGC10870.2170388473579963No Hit
GTCACACCCGGTTGT10490.20945147274934509No Hit
ATAACCGAATATGGA10460.20885246949076736No Hit
TTCAGTCTAGTGACT10090.20146476263497537No Hit
AGCAGGTATTTATTG9900.1976710753306498No Hit
TCTGGAGTAACAAGC9870.19707207207207206No Hit
CACCTAGACGGGGGC8970.1791019743147403No Hit
CACCTAGACGGCGGC8950.1787026388090218No Hit
TGGAGCATAACGTAT8190.16352788959171938No Hit
ATAGCGAGCACTCAC7600.1517474921730241No Hit
TCCTGTAGCGTTGGG7550.15074915340872788No Hit
GGCGATTGGCTTCTT7510.1499504823972909No Hit
AGGCTAGTGCGGGAT7180.14336144655293592No Hit
AACAGCTTGTGCCAC6720.13417672992141078No Hit
AGTGAGCCAGCTATA6640.13257938789853682No Hit
ATCCAGGTGGTGGCA6620.13218005239281835No Hit
ATGACGTTAGAGTAG6560.1309820458756629No Hit
AAGCCTTTTGATGGT6440.12858603284135198No Hit
CTTCGAATGGCAGAG6380.12738802632419655No Hit
CCTGACTTTATTAGG6360.12698869081847805No Hit
AACCTTGGAGGGCCT6350.12678902306561882No Hit
ATAACCGGCCGGTAC6270.12519168104274486No Hit
GGTATCACCTGACTC6160.1229953357612932No Hit
ACGCTTCAGTATTAT6070.12119832598556005No Hit
CACCTAGCCGGTGGC5950.11880231295124913No Hit
AAGACTCCTCTGCTT5900.1178039741869529No Hit
AGCCTTCCTCGGGCC5790.11560762890550123No Hit
CGGATGTCCGCTTTT5780.11540796115264201No Hit
CTGATCAGTCAATGG5760.11500862564692352No Hit
GGCTAGAGATGGAAA5760.11500862564692352No Hit
GCTACCATGGTTCGT5730.11440962238834579No Hit
AGCTAGTCGTCGGTC5700.11381061912976806No Hit
CGAGAGATAGACTTG5640.11261261261261261No Hit
GTCGAGAACGGGTGT5590.1116142738483164No Hit
CTAAGCGCCAGTGTG5430.10841958980256854No Hit
AGTCTCCAGGATGTT5390.10762091879113155No Hit
ACTACTGGGCAAGCC5100.10183055395821354No Hit
TATCGCGGGGAGGAG5100.10183055395821354No Hit
ACGTCAAGAGGAGGT5080.10143121845249504No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)