FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002271348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217107
Sequences flagged as poor quality0
Sequence length15-160
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA9832845.29011040638948No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA108194.983257103640141No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC83413.84188441643982No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG46892.1597645400654977No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA22581.0400401645271686No Hit
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC22181.0216160694956864No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC17420.8023693386210485No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA14800.6816915161648404No Hit
GATAACTTCCAATCCAGGAGGCCGGCACGGAGGTGGTCAAGGCTAAAGCC14240.6558977831207654No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC13990.644382723726089No Hit
CAGACCCCAGGACCGCTCCAAGCAGTTCATGCACAGCCGTGGCCTCCGCA12170.5605530913328451No Hit
CCTGAATGCCCCAACAGCTAATCAGTAGAGACCTGGCGAGCATTTTACTT11940.5499592366897429No Hit
CAGACCCCAGGACCGGCCGAACGCAGAGGTTGATTCTTCACCACACTGAA10590.48777791595849046No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC9410.4334268356156181No Hit
CAGACCCCAGGACCAATCCCTGTCACCATCTTGATGAAGAGGCAGCGCCA8830.40671189781996897No Hit
GATCCATCTCCACTCTGACTGTGAGCAACATGAGCCCTGAAGACAGCAGC8180.37677274339381045No Hit
GTTTCACATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA8120.3740091291390881No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA8070.37170611726015285No Hit
GTTTCCCATCAGCCGCCCAAACCGTGGCAGCAGGACCGACAAGTCAAGTG8040.37032431013279166No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA7690.35420322698024476No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGCGCA7570.3486759984708001No Hit
CGGTTCTTTGCAGTCAGGCCTGACTGGCATTGTATTAGCAAACTCATCAC7050.3247246749298733No Hit
CGATTCTCAGGGCGCCTGGGACACCGGCTTTCTAACATTTTTACAGCTCA6970.32103985592357687No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG6520.3003127490131594No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC6200.2855734729879737No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA6030.27774323259959377No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG5770.26576757082913033No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGC5510.25379190905866694No Hit
CAGACCCCAGGACCCATTAAGCCACCCCCGTGTTCCTGCCGTCAGTGCCA4980.22937998314195304No Hit
CAGACCCCAGGACCGGCTGCAACCCCACCTCCTGGAGATGCTGATTCAGC4970.228919380766166No Hit
GTTTCCCATCAGCCGCCCAACCCTAACATTCTCAACTCTGACTGTGAGCA4080.18792576932111815No Hit
NNNNNNNNNNNNNNN4050.186543962193757No Hit
GTTTCCCATCAGCCGCCAAACCTAACATTCTCAACTCTGACTGTGAGCAA3890.1791743241811641No Hit
CGATTCTCAGGGCGCCCAGTGAAGAAATCTATACGAGATACTCCAGCCAA3830.1764107099264418No Hit
CAGACCCCAGGACCGCCACCTCTTCCTGTTCAACAGCATGATCCTTTACT3730.17180468616857125No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGCCTGTGAGCA3710.17088348141699714No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG3700.1704228790412101No Hit
GTTTCCCATCAGCCGCCCACACCTAACATTCTCAACTCTGACTGTGAGCA3580.16489565053176544No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCCACTCTGACTGTGAGCA3540.16305324102861724No Hit
GTTTACCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA3530.16259263865283016No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTTACTGTGAGCA3330.1533805911370891No Hit
CAGACCCCAGGACCAGCTGACAGCACTCACTGGGCGGATCCAGGAGGCCG3310.15245938638551498No Hit
CAGACCCCAGGACCGGCTTCTGGGCGGCTGAAAGGGGCAAGGAGGCAAGG3270.15061697688236675No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG3230.14877456737921854No Hit
CAGATGGCTATAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG3220.1483139650034315No Hit
GGAACGATTTTCTGCTGAATTTCCCAACCCACCCCCAAACTAAACACTAA3170.14601095312449622No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG3150.1450897483729221No Hit
CAGACCCCAGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG3130.14416854362134798No Hit
GTTTCCCATCATCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA3120.14370794124556094No Hit
GTGTCTCCAGAGCAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCT3110.1432473388697739No Hit
GTTTCCCATCAGCCGCCCAAACCTACCATTCTCAACTCTGACTGTGAGCA3060.14094432699083861No Hit
GTTTCCCATCAGCCGCCCAAACCTAACCTTCTCAACTCTGACTGTGAGCA3030.13956251986347745No Hit
GTTTCCCATCAGCCGCACAAACCTAACATTCTCAACTCTGACTGTGAGCA3020.1391019174876904No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGGGAGCA2910.1340352913540328No Hit
CAGATGGCTACAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG2860.13173227947509755No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGATCA2670.1229808343351435No Hit
CCTGAATGCCCCAACAGCCGCCCAAACCTAACATTCTCAACTCTGACTGT2650.12205962958356939No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCA2570.11837481057727296No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC2500.11515059394676358No Hit
CGATTCTCAGGGCGCTCTCCCAGCAAAGCAGTGGCCGCCCGGGCGGCGGG2460.11330818444361536No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTTAGCA2400.11054457018889302No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGAGAGCA2350.10824155830995777No Hit
GTTTCCCATCAGCCGCCCAAACCTAAAATTCTCAACTCTGACTGTGAGCA2300.10593854643102249No Hit
GTTTCCCATCAGACGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA2260.10409613692787427No Hit
GTTTCCCATCAGCAGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA2200.10133252267315195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)