FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93053
Sequences flagged as poor quality0
Sequence length15-160
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA2569427.6122209923377No Hit
CGGTTCTTTGCAGTCAGGCCTGTTATAACCGCTGAGCAGCCACACTCGCA29273.1455192202293314No Hit
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC24232.6038924054033723No Hit
GATAACTTCCAATCCAGGAGGCCGGCACGGAGGTGGTCAAGGCTAAAGCC17041.8312144691734817No Hit
CGATTCTCAGGGCGCCATCGCCAAGTAAGAAAGTGAAGCAAATCAGAAAC13291.4282183271898812No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC12111.3014088745123749No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA10221.09829881895264No Hit
CCTGAATGCCCCAACAGCTAATCAGTAGAGACCTGGCGAGCATTTTACTT9351.0048037140124446No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG7990.8586504465197254No Hit
CCTGAATGCCCCAACAGCTCTCACGGCTGCTGCTCCTCTGGACTCCCCAA7460.8016936584527098No Hit
GTTTCCCATCAGCCGCCCAAACCGTGGCAGCAGGACCGACAAGTCAAGTG6760.7264677119491043No Hit
CAGACCCCAGGACCGGCCCCATCATTGAGGAGGTTGATTGAATGGCCCTT5870.6308232942516631No Hit
CGGTTCTTTGCAGTCAGGCCTGACTGGCATTGTATTAGCAAACTCATCAC5540.5953596337571062No Hit
CAGACCCCAGGACCAGGCTCAGAGGGACATCATATTTGAACTTCGAAGAA4830.5190590308748777No Hit
CGATTCTCAGGGCGCCTGGCTTGAGAGTTGAGAGGCTAAGTGTGCAATGT4730.5083124670886484No Hit
CAGACCCCAGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG4500.4835953703803209No Hit
NNNNNNNNNNNNNNN4190.4502810226430099No Hit
GTTTCCCATCAGCCGCCCAAACCGTAGTGGCACTGGACCATGTTGCCCGG3810.40944408025533835No Hit
CAGACCCCAGGACCGTGCAGCATATAAAGAGTACATCTCCAATAAACGTA3620.3890256090615026No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG3510.3772043888966503No Hit
CGATTCTCAGGGCGCCCAGTGAAGAAATCTATACGAGATACTCCAGCCAA3370.3621591995959292No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGCGCA3250.349263323052454No Hit
GTTTCACATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA3160.33959141564484757No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG3150.3385167592662246No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG3110.33421813375173287No Hit
CAGACCCCAGGACCGACCAAAGCCCGCGCGCCGCTGCATCCCGCGTCCAG3070.32991950823724114No Hit
CAGACCCCAGGACCAGCTGACAGCACTCACTGGGCGGATCCAGGAGGCCG2890.3105756934220283No Hit
GATAACTTCCAATCCAGGAGGCCACAGTGTAAGCAATAACAGATCTGCCA2770.2976798168785531No Hit
GTTTCCCATCAGCCGCCCAAACCTCAGCACCACACAAATATCCTGTAACA2700.2901572222281925No Hit
GGTACAGCGTCTCTCGGGACAACTGGCACAAGCGCCGCAAAACCGGGGGC2560.27511203292747144No Hit
CAGACCCCAGGACCGGCCTTTCTGACAAGAGCTAGACTTCGGGCTCCTTG2520.2708134074129797No Hit
CGATTCTCAGGGCGCTCTCCCAGCAAAGCAGTGGCCGCCCGGGCGGCGGG2510.26973875103435674No Hit
CAGACCCCAGGACCCGATGCCTTCACTGCTGAATTCTGTCAAACATTTAA2480.266514781898488No Hit
GATCCATCTCCACTCTGACTGTGAGCAACATGAGCCCTGAAGACAGCAGC2410.2589921872481274No Hit
GATAACTTCCAATCCAGGAGGCAGTGGAGGAAGTGGGGGTTATGGTGGGA2370.25469356173363566No Hit
CTCAGCAGAGATGCCTGATGGTTCAAGTAGAGGCCCAGTACATCTTGATC2350.2525442489763898No Hit
GATAACTTCCAATCCAGGAGGCCGCCAAGAAGAAGCCAGGCATCTTCATC2300.24717096708327513No Hit
CGATTCTCAGGGCGCCTACCAGGAGGGCGTGCTGCTGCTGAACAAGGCCC2230.23964837243291456No Hit
GTCTCCAGATCAACCACAGAGGATGCTCAGGCCGCAGTGGATTATTACAC2110.22675249588943935No Hit
CAGACCCCAGGACCGCAGCAGCAGCCAGGTGCCGAGTGGATTGAGTGCCA1930.20740868107422653No Hit
GATAACTTCCAATCCAGGAGGCTGAGGGAGGAGGATGGCTTGAACCCAGG1880.20203539918111185No Hit
GTTTCCCATCAGCCGCCCAACCCTAACATTCTCAACTCTGACTGTGAGCA1860.19988608642386596No Hit
CGATTCTCAGGGCGCCCAACGATTAACTGAATATTCACGAACACTGAGTG1850.19881143004524304No Hit
CCTGAATGCCCCAACAGCCCTCCTGTGCCTGCTTCCCTGTGTGTCGGGGT1820.19558746090937423No Hit
GTTTCCCATCAGCCGCCCACACCTAACATTCTCAACTCTGACTGTGAGCA1740.18699020988039075No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGCCTGTGAGCA1730.1859155535017678No Hit
GTTTCCCATCAGCCGCCCAAACCTGCCCTTCCTCTTCTAGCCCCATTTTC1730.1859155535017678No Hit
CGATTCTCAGGGCGCCACCCTTGACGCTTGCAGCTTCGGAGTCACGGGTT1650.17731830247278432No Hit
CAGACCCCAGGACCGACGGGAAGGTTTTCCAGTTTCTTAATGCGAAATGC1570.16872105144380084No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC1560.16764639506517792No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG1550.16657173868655498No Hit
CCTGAATGCCCCAACAGCTTCATCCTGGGTCTGAAGCAGCAGATTGAAGA1450.15582517490032563No Hit
CCTGAATGCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCAC1400.15045189300721096No Hit
GTTTCCCATCAGCCGCCCAAAGTGCTGGGATTACAGGCCTGAGCCACCAC1400.15045189300721096No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTTACTGTGAGCA1370.14722792387134215No Hit
CTCCGCACAACAGTTCCCTGACCAAGGGCAAGGTGTGTCTCAGGAAGGTG1360.1461532674927192No Hit
CCTGAATGCCCCAACAGCTCATTGAGAACGGGCCATGATGACGATGGTGG1340.14400395473547334No Hit
CTCCGCACAACAGTTCCCTGACTTTGACACACATGGCCACCTTGGCACAA1310.14077998559960453No Hit
CGATTCTCAGGGCGCCCAACGATCTGTAAAGGTGTCTTCCACGCTATAGT1300.13970532922098158No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCCACTCTGACTGTGAGCA1290.13863067284235867No Hit
GTTTCCCATCAGCCGCCCAAACCTACCATTCTCAACTCTGACTGTGAGCA1290.13863067284235867No Hit
CTCAGCAGAGATGCCTGATGCCAAGGCACTGGCAAAATTGTTGGTAGGCT1270.13648136008511277No Hit
GTTTACCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA1270.13648136008511277No Hit
CGATTCTCAGGGCGCCAAGCAGTGACTTCTGACCAACAGAGTGTGGGGAG1270.13648136008511277No Hit
CAGACCCCAGGACCGGCTTCTGGGCGGCTGAAAGGGGCAAGGAGGCAAGG1230.13218273457062105No Hit
GTTTCCCATCAGCCGCCCAAACCTAACCTTCTCAACTCTGACTGTGAGCA1230.13218273457062105No Hit
CTCAGCAGAGATGCCACAAACAGGCTGTCCTCCAGAGCCAGCTCCCCCAC1200.12895876543475224No Hit
CGATTCTCAGGGCGCCAGTCCTATACTCTTAGACAGCTGGATAATGCTGT1180.12680945267750637No Hit
CCTGAATGCCCCAACAGCTCTCTTCCTGCTAACATCTCAGGCTCCCAGCC1170.12573479629888343No Hit
GTTTCCCATCAGCCGCCCCAACCTAACATTCTCAACTCTGACTGTGAGCA1130.12143617078439169No Hit
CAGACCCCAGGACCGGCGAAGAGAAAAGTCTCCAGCAAAACAAAAATAAT1110.11928685802714582No Hit
GTTTCCCATCAGCCGCCAAACCTAACATTCTCAACTCTGACTGTGAGCAA1110.11928685802714582No Hit
CGGTTCTTTGCAGTCAGGGCGCAAAGGAACTGACATCATGTACACAGGCA1110.11928685802714582No Hit
GTTTCCCATCAGCCGCACAAACCTAACATTCTCAACTCTGACTGTGAGCA1080.11606288889127701No Hit
CTCAGCAGAGATGCCTGATGCAGCAAAAAAGGGAGTTGTCAAGGCTGAAA1080.11606288889127701No Hit
GCTGAAAGGACTGGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA1070.11498823251265408No Hit
GATAACTTCCAATCCAGGAGGCCATGGAGGAGAGCAAGACACACTTCCGC1070.11498823251265408No Hit
CGATTCTCAGGGCGCCCACGAATAAAAAAAGATGAGAAGCAGAACTTATT1070.11498823251265408No Hit
CGATTCTCAGGGCGCCCAACGATGTAAACTTTACAATATCTAGAATCTAA1060.11391357613403114No Hit
GTTTCCCATCATCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA1050.1128389197554082No Hit
CAGACCCCAGGACCGGCTGACAGCACTCACTGGGCGGATCCAGGAGGCCG1040.11176426337678527No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCGGACTGTGAGCA1040.11176426337678527No Hit
GTTGTCTCCAGATCCACCAACAGCAACTTTTCTAAGACCTCTCCTAAGGC1040.11176426337678527No Hit
CGATTCTCAGGGCGCCCAGTGACACCATCGAAAATGTGAAGGCCAAGATC1040.11176426337678527No Hit
GTTTCCCATCAGCCGCCCAAACCACCAACCGCCAGCAGGGTCAGTGGCAG1030.11068960699816234No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGGGAGCA1020.10961495061953941No Hit
GTTTCCCATCAGCCGCCCAAACCAGGGCTATGAAGCAGGTAGCAGGTACC1010.10854029424091646No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTTAGCA1000.10746563786229353No Hit
CGGTTCTTTGCAGTCAGGCCCATGATAGTCAGTGCAGTTGGGGCAGTTGT1000.10746563786229353No Hit
CCTGAATGCCCCAACAGCTCTCCTGTGCCTGCTTCCCTGTGTGTCGGGGT1000.10746563786229353No Hit
CAGACCCCAGGACCGGGCTCAGAGGGACATCATATTTGAACTTCGAAGAA990.1063909814836706No Hit
CGATTCTCAGGGCGCCCAACGATATTCAAAGGAAATGTTTATTGAAACAT980.10531632510504765No Hit
GTTTCCCATCAGACGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA980.10531632510504765No Hit
CGATTCTCAGGGCGCCCAGTGAAGAAAGGACAAGAATCCTTCAAGAAACA980.10531632510504765No Hit
CAGACCCCAGGACCAGCGAGCGCAGGGGGCAGTGCTGAGGCCCTGCTGAG960.10316701234780179No Hit
CAGACCCCAGGACCTGTGCAATCAAATATTGTGGAGAATTCCCTAGCTGG960.10316701234780179No Hit
GTTTCCCATCAGCCGCCCAAACCTGGAGACCTGATTGAGATTTTCCGCCT950.10209235596917886No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGATCA940.10101769959055593No Hit
CGGTTCTTTGCAGTCAGGCCCCCGCACACCCAACCCCTATGCCTCGGACA940.10101769959055593No Hit
CGGTTCTTTGCAGTCAGGCTTCATACGCTATTGTCCTGCCCGTTAGAGCA940.10101769959055593No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)