FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002271353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056837
Sequences flagged as poor quality0
Sequence length15
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTGCCGTACGTAG65967362.419559496876055No Hit
NNNNNNNNNNNNNNN496484.6977916178180745No Hit
ATGTGCCGGACGTAG183861.739719559402254No Hit
GCTAGAATTTTGTTC111571.0556973308088191No Hit
ACACGTGGCGAGACC106431.0070616376981503No Hit
TCACAGTTTAGACTC103130.9758363872574484No Hit
ATGTGCCGTACGCAG102000.9651441045307838No Hit
ATGTGCCGTACGTCG81280.7690873805515893No Hit
ATGTGCCGTACGGAG70770.6696396889965056No Hit
ATGTGCCGTCCGTAG49510.4684733785815599No Hit
ATGTGCCGTACGTAT36270.3431938889346228No Hit
ATGTGCCGTACGAAG35050.3316500084686664No Hit
ATGAGCCGTACGTAG32650.30894073542088324No Hit
ACGTGCCGTACGTAG30460.28821852376478113No Hit
ATCCAGGTTGTTGAG29530.27941868045876517No Hit
ATGGGCCGTACGTAG28930.2737413621968194No Hit
GAGGATCGTAACTCA26280.2486665398732255No Hit
GTGTGCCGTACGTAG26140.2473418322787715No Hit
AAGGTACGCGAAGGG24810.2347571101314583No Hit
ATGTGCCGTACGTTG23940.2265249986516369No Hit
ATGTGCCTTACGTAG22100.2091145559816698No Hit
ATGCGCCGTACGTAG20340.1924610890799622No Hit
CTAGGGTGTACTGAG20030.18952780797795687No Hit
ATGTGCCGTAAGTAG16770.15868104542138475No Hit
TCACAGGTTAGACTC15710.1486511164919472No Hit
GCACAGTTTAGACTC14410.13635026025773134No Hit
ATGTGCCGTTCGTAG14090.13332235718469357No Hit
AGGTGCCGTACGTAG12280.11619578042782379No Hit
ATAGGTCTTATGTTT11780.1114646818762023No Hit
ACACGTGGCGAGCCC11340.10730131515077539No Hit
GCTAGCATTTTGTTC11170.10569274164322408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)