FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002271354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184077
Sequences flagged as poor quality0
Sequence length15-160
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA5488629.816870114137018No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC142357.7331768770677485No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC49092.666818776924874No Hit
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC46872.54621707220348No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG44022.3913905593854747No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA39522.1469276444096765No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC31411.7063511465310714No Hit
CAGACCCCAGGACCGGCCCATGTCCCCCGGAGACTGTCCTCCGGAAACAA25921.4081063902605975No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC25261.3722518293974804No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT19611.0653150583723114No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC15720.8539904496487883No Hit
GTTTCCCATCAGCCGCCCAAACCTTAGAAAGCTGGATGATACAGACCCTG14560.7909733426772493No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA14060.7638107965688271No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGC9950.5405346675575982No Hit
CGGTTCTTTGCAGTCAGGCCTGACTGGCATTGTATTAGCAAACTCATCAC9310.5057666085388179No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC8570.46556604029835347No Hit
CGATTCTCAGGGCGCCATCGCCAAGTAAGAAAGTGAAGCAAATCAGAAAC8280.44981176355546865No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG7130.387337907506098No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG6520.3541996012538231No Hit
CAGACCCCAGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG6320.3433345828104543No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG6060.32921005883407484No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCA5780.3139990330133585No Hit
CAGATGGCTATAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG5590.3036772654921582No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGCGCA5280.2868364869049365No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT5260.2857499850605997No Hit
CAGATGGCTACAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG5050.2743417156950624No Hit
GTGTCTCCAGAGCAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCT4870.26456319909603043No Hit
GATCCATCTCCACTCTGACTGTGAGCAACATGAGCCCTGAAGACAGCAGC4700.255327933419167No Hit
GTTTCACATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA4580.2488089223531457No Hit
GGAACGATTTTCTGCTGAATTTCCCAACCCACCCCCAAACTAAACACTAA4530.24609266774230348No Hit
CAGACCCCAGGACCGGCCCCATCATTGAGGAGGTTGATTGAATGGCCCTT4500.2444629149757982No Hit
CAGACCCCAGGACCGGCGCAAGGAGGTCACGGATGAGATAGTGAAAGAGT4340.2357709002211031No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG4150.22544913269990277No Hit
CAGACCCCAGGACCGGCTTCTGGGCGGCTGAAAGGGGCAAGGAGGCAAGG3680.19991633935798606No Hit
CGATTCTCAGGGCGCCCAGTGAAGAAATCTATACGAGATACTCCAGCCAA3480.18905132091461724No Hit
NNNNNNNNNNNNNNN3310.17981605523775376No Hit
CCTGAATGCCCCAACAGCTCAGTGCTTTCCTGACACTCCAGTGTCTGGGG3170.1722105423273956No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG3080.16732128402787963No Hit
GTTTCCCATCAGCCGCCCACACCTAACATTCTCAACTCTGACTGTGAGCA2950.16025902203968992No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGCCTGTGAGCA2900.1575427674288477No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG2800.1521102582071633No Hit
GTTTCCCATCAGCCGCCCAACCCTAACATTCTCAACTCTGACTGTGAGCA2750.1493940035963211No Hit
CCTGAATGCCCCAACAGCTCTGTATAGGAGATAAGGATTGCTGACTCCAC2560.13907223607512073No Hit
GGCTACAATGTATCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCT2500.1358127305421101No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGGGAGCA2360.12820721763175194No Hit
GTTTCCCATCATCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA2360.12820721763175194No Hit
GTTTCCCATCAGCCGCCAAACCTAACATTCTCAACTCTGACTGTGAGCAA2360.12820721763175194No Hit
GTTTCCCATCAGCCGCCCAAACCTACCATTCTCAACTCTGACTGTGAGCA2360.12820721763175194No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCCACTCTGACTGTGAGCA2350.1276639667095835No Hit
CAGACCCCAGGACCCATTAAGCCACCCCCGTGTTCCTGCCGTCAGTGCCA2310.12549096302090973No Hit
GTTTCCCATCAGCCGCCCAAACCTAACCTTCTCAACTCTGACTGTGAGCA2290.12440446117657285No Hit
GTTTACCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA2160.11734219918838312No Hit
GGGGTACAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC2130.11571244642187781No Hit
CAGACCCCAGTCCACCATGATCCATCTGGGTCACATCCTCTTCCTGCTTT2060.11190968996669871No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG1990.10810693351151962No Hit
CGGTTCTTTGCAGTCAGGCCTGGGTGGAGCCCAGGTTCTGCTCATCACTC1980.1075636825893512No Hit
CAGACCCCAGGACCGACGGGAAGGTTTTCCAGTTTCTTAATGCGAAATGC1960.10647718074501432No Hit
GATAACTTCCAATCCAGGAGGCCACGGTCTGAGGAACAACCAAACCCATG1920.10430417705634056No Hit
CCTGAATGCCCCAACAGCTAATCAGTAGAGACCTGGCGAGCATTTTACTT1870.10158792244549836No Hit
CCTGAATGCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCAC1850.10050142060116148No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)