FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002271358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255003
Sequences flagged as poor quality0
Sequence length15-160
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA12933150.717442539891685No Hit
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA104694.105441896761999No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG104674.104657592263621No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC87223.4203519174284223No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA43791.7172346991996175No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG31641.2407697164347087No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCA25781.0109684984098226No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA24990.9799884707238739No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC19020.7458735779579064No Hit
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC18980.7443049689611495No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC15550.6097967474892452No Hit
CAGACCCCAGGACCGGCCGAACGCAGAGGTTGATTCTTCACCACACTGAA15160.5945028097708654No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC14230.5580326505962675No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGCGCA9120.3576428512605734No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCTTGATTCTGG8690.34078030454543673No Hit
GATCCATCTCCACTCTGACTGTGAGCAACATGAGCCCTGAAGACAGCAGC8490.3329372595616522No Hit
GTTTCACATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA7440.2917612733967836No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA7340.28783975090489133No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG7120.27921240142272835No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG7110.2788202491735391No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGC6280.24627161249083343No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG6270.2458794602416442No Hit
GTTTCCCATCAGCCGCCAAACCTAACATTCTCAACTCTGACTGTGAGCAA5970.23411489276596747No Hit
CGGTTCTTTGCAGTCAGGCCTGACTGGCATTGTATTAGCAAACTCATCAC5430.2129386713097493No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG5380.21097791006380318No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC5170.20274271283082945No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG5080.1992133425881264No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG4930.19333105885028803No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATC4660.18274294812217895No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT4480.1756842076367729No Hit
GTTTCCCATCAGCCGCCCACACCTAACATTCTCAACTCTGACTGTGAGCA3750.14705709344595946No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGATTTCCCCCTGATCCTGGAGTCG3680.1443120277016349No Hit
GTGTCTCCAGAGCAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCT3590.14078265745893187No Hit
CAGATGGCTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT3560.13960620071136418No Hit
GCTGAAAGGACTGGAGGGACGCAAGGCGTTTCTGATGAAGCCGATACATT3520.13803759171460728No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC3510.13764543946541805No Hit
CAGATGGCTATAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG3500.13725328721622881No Hit
GTTTCCCATCAGCCGCCCAACCCTAACATTCTCAACTCTGACTGTGAGCA3480.13646898271785038No Hit
GATAACTTCCAATCCAGGAGGCCGGCACGGAGGTGGTCAAGGCTAAAGCC3460.13568467821947192No Hit
CAGACCCCAGGACCGCGCCCCCAGAGATTTCTGCGTCAAGTTCCCTGAGG3450.1352925259702827No Hit
CCTGAATGCCCCAACAGCCGCCCAAACCTAACATTCTCAACTCTGACTGT3410.1337239169735258No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA3380.13254746022595812No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGCCTGTGAGCA3350.13137100347839045No Hit
CAGACCCCAGGACCGGCCCCATCATTGAGGAGGTTGATTGAATGGCCCTT3240.127057328737309No Hit
CAGACCCCAGGACCGGCAGCTGACCCTGAGCGGGATCTACGCCCACATCA3190.12509656749136283No Hit
CAGATGGCTACAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG3180.12470441524217361No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTTACTGTGAGCA3090.1211750449994706No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA3030.11882213150433525No Hit
GTTTCCCATCATCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA3020.11842997925514602No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCCACTCTGACTGTGAGCA3010.1180378270059568No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG2870.11254769551730763No Hit
GTCTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA2780.10901832527460462No Hit
GTTTCCCATCAGCCGCCCAAACCTAACCTTCTCAACTCTGACTGTGAGCA2750.10784186852703694No Hit
CAGATGGCTATAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT2710.10627325953028004No Hit
GTTTACCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA2570.10078312804163089No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)