FastQCFastQC Report
Wed 14 Sep 2022
EGAF00002271412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34277287
Sequences flagged as poor quality0
Sequence length68
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCC2391840.6977915142467372No Hit
TCCCCGCGTCGACGGATCCGTTGGATAGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG1671150.48753858495277064No Hit
ATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGG1428390.4167161771000138No Hit
TCCCCGCGTCGACGGATCCGCCAACTTGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG1268990.3702130801658836No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1043400.3043998202074744No Hit
GCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACC940800.27446746295878083No Hit
TCCCCGCGTCGACGGATCCTGTCTAGTGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG840930.24533155147313732No Hit
ACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCC733870.21409804107308728No Hit
GGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTT698380.20374424615343684No Hit
TCCCCGCGTCGACGGATCCGGGGGACGGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG687460.20055846310123668No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCC686130.20017045106282771No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA676270.19729391068785576No Hit
TCCCCGCGTCGACGGATCCAAGTTGCAGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG661660.19303161303285177No Hit
AGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGC630150.18383893684468086No Hit
TCCCCGCGTCGACGGATCCATTACTGGGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG615750.17963790424837298No Hit
TCCCCGCGTCGACGGATCCGCTGAACGGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG592280.17279080459314064No Hit
ATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCG583330.1701797461391854No Hit
TCCCCGCGTCGACGGATCCCTCGTGTGGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG554810.16185936769149786No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG553860.16158221623549146No Hit
ATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA532000.1552048153635963No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTC523030.1525879221421462No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGT488740.1425842132721881No Hit
TCCCCGCGTCGACGGATCCTAGAGGTGGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG482920.14088629593118032No Hit
TCCCCGCGTCGACGGATCCTCGAGAGGGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG473790.13822272456977125No Hit
AATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAAT472900.1379630774162494No Hit
GCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCCC466340.1360492736779314No Hit
TCCCCGCGTCGCCATGCCTCGGAAAATTGAGGAGATCAAGGACTTTCTGCTGACAGCCCGGCGGAAGG436920.12746633069297464No Hit
ATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATA413450.12061923103774227No Hit
TTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGC412030.12020496254560636No Hit
TCCCCGCGTCGACGGATCCCTTGTTCCGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG404700.11806652025873578No Hit
AATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGG395060.11525416232620743No Hit
ATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGA379640.11075555658766109No Hit
TCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGC378210.11033837071177774No Hit
CTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCT364300.10628028991909425No Hit
GCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCC361300.10540507479486344No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG355660.10375967036130951No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCG354820.10351461012652488No Hit
TCCCCGCGTCGACGGATCCATTTTGAGGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG347210.10129448109472608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)