FastQCFastQC Report
Tue 27 Sep 2022
EGAF00002271413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29438942
Sequences flagged as poor quality0
Sequence length68
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCCGCGTCGACGGATCCGGGCGGGAGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG1794830.6096788396811271No Hit
GTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCC1736260.5897834236026552No Hit
TCCCCGCGTCGACGGATCCTTTGGTGGGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG1258950.427647841420388No Hit
TCCCCGCGTCGACGGATCCGCCAACTTGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG1129890.3838079507069242No Hit
TCCCCGCGTCGACGGATCCTGTCTAGTGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG974680.3310852679420341No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT943830.3206059511241946No Hit
GGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTT883580.3001398623632602No Hit
ATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGG880320.2990324856103864No Hit
TCCCCGCGTCGACGGATCCAAGTTGCAGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG771510.26207123883731964No Hit
GCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACC747740.2539968997527153No Hit
TCCCCGCGTCGACGGATCCCTCGTGTGGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG633720.21526588829177354No Hit
TCCCCGCGTCGACGGATCCGACAGTCCGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG553910.18815553901359636No Hit
TCCCCGCGTCGACGGATCCTAGAGGTGGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG494310.16791024623099565No Hit
TCCCCGCGTCGACGGATCCGCTGAACGGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG483820.16434693882680973No Hit
TCCCCGCGTCGCCATGCCTCGGAAAATTGAGGAGATCAAGGACTTTCTGCTGACAGCCCGGCGGAAGG464130.15765851911390022No Hit
TCCCCGCGTCGACGGATCCATTTTGAGGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG456970.15522636649102403No Hit
AGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGC392860.13344908930490776No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA389510.13231114080118775No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCC387870.13175405556354572No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGT387820.13173707125752007No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG387800.13173027753510977No Hit
TCCCCGCGTCGACGGATCCGGGGGACGGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAG365350.1241043241295832No Hit
GCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCCC361070.12265046753378568No Hit
CTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCT348680.11844175650062425No Hit
ATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCG342910.11648176758526171No Hit
ACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCC340590.11569369578567056No Hit
TCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGC337580.11467124056292512No Hit
CCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGT337510.11464746253448917No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTC335140.11384240642887235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)