FastQCFastQC Report
Wed 14 Sep 2022
EGAF00002273187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002273187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32124138
Sequences flagged as poor quality0
Sequence length16-125
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGCCGGG984940.3066043359669293No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA915560.28500686928937985No Hit
CGGACGGCGCCGGACGCGCACGCCCCGCCGGGCCCCCCGCACGCACGCGC860450.2678515451527446No Hit
GACGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGCCGGGCCCC852230.2652927216288263No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC828610.2579399951525548No Hit
CGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTA764930.238116895152175No Hit
CACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTT660040.20546543536825798No Hit
CCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACG630170.1961671313950899No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA619510.1928487544163831No Hit
ACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTC582850.18143677505058656No Hit
CCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAA570430.1775705234487537No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT566610.17638138648265053No Hit
TCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTC540610.16828778409556078No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCA537310.16726051917719942No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCA534450.16637022291461953No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC533310.1660153495791856No Hit
CGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGG532490.16576008981159276No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA530300.1650783594566802No Hit
GGACGGACGGCGCCGGACGCGCACGCCCCGCCGGGCCCCCCGCACGCACG508890.15841358918331133No Hit
GTGCGGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCG499110.15536914951616757No Hit
CAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCT493770.15370684810281915No Hit
GGGAGGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGC482350.15015188890048972No Hit
CTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGG479150.14915575322207866No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT474760.14778917958825852No Hit
CCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAG469990.14630431484262707No Hit
CGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGG459190.1429423569279898No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG457250.1423384496729531No Hit
CAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGT455780.14188084984568303No Hit
GTTCTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGC447790.13939362357365045No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA435570.13558963045171826No Hit
CGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA419000.13043151539194608No Hit
CCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACG415400.12931086275373366No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACC413800.12881279491452813No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA409150.12736528525683707No Hit
CTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTG407640.12689523373358688No Hit
GTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCAC400510.12467571892512727No Hit
CGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGCCGGGCCCCCC394560.12282352914808174No Hit
CGGCGGTGAACGGGGAGGAGGCGGGAACCGAAGAAGCGGGGCGCGCCGAC380820.11854637157890431No Hit
AGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGG380070.11831290227927672No Hit
CCCGCACGCACGCGCGCGCGCGCGCGCGGACAAACCCTTGTGTCGAGGGC374620.11661635870198292No Hit
GTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGC370410.11530581770007339No Hit
GTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGC366620.1141260195059553No Hit
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCT360260.11214619984511334No Hit
GACAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAG355210.11057417322762092No Hit
CCGGACCCCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGG354290.11028778422007776No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC348010.10833286795119608No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCT345080.10742078122065098No Hit
CACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCAT344600.1072713608688893No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGA343310.10686979367352985No Hit
CCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGA342010.10646511355417536No Hit
CCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACG339610.10571801179536709No Hit
CATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTA335930.10457245576519437No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC335560.1044572775773781No Hit
CAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAAC329370.10253037762445175No Hit
GTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGC329130.1024556674485709No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC328680.10231558586879437No Hit
CGGACCCCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGG326930.10177082416966332No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGG323430.10068130077140125No Hit
ACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGAC322950.10053188041963959No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)