FastQCFastQC Report
Wed 22 Feb 2023
EGAF00002273189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002273189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32519990
Sequences flagged as poor quality0
Sequence length16-125
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1017190.3127891490741541No Hit
GGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGCCGGG925930.28472640981746916No Hit
GACGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGCCGGGCCCC803650.247124922240136No Hit
CGGACGGCGCCGGACGCGCACGCCCCGCCGGGCCCCCCGCACGCACGCGC762800.23456341776242862No Hit
GTGCGGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCG656520.2018819808985181No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA642350.19752466098544313No Hit
CCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACG623880.19184507744313575No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG583740.17950190021583648No Hit
CCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAA568140.1747048507702493No Hit
CCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAG567610.17454187409036717No Hit
CGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTA547160.16825343427227377No Hit
CGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGG537420.16525835340047768No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG508920.15649451306719342No Hit
CTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTG462720.14228786663218532No Hit
GGGAGGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGC459710.14136228209172266No Hit
CGGCGGTGAACGGGGAGGAGGCGGGAACCGAAGAAGCGGGGCGCGCCGAC458570.14101172847839127No Hit
CGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGG451050.1386993046430826No Hit
GTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGC450540.13854247802659225No Hit
GGACGGACGGCGCCGGACGCGCACGCCCCGCCGGGCCCCCCGCACGCACG444620.13672205926262584No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA435800.13400988130685157No Hit
CCGGACCCCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGG435620.13395453073632557No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC421840.12971713705939023No Hit
GTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGC410990.12638072766935046No Hit
CCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACG409690.12598097354888485No Hit
CGGACCCCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGG405140.12458183412725526No Hit
CACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTT392310.12063656846142941No Hit
GGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTT385240.11846252105243574No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCA383280.11785981484004146No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA376340.11572574284309436No Hit
CAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCT372590.1145726059571359No Hit
CAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGT370290.11386534866708138No Hit
GGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGG366010.1125492351012408No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCA365920.11252155981597779No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG363230.11169437628978361No Hit
TCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTC362970.11161442546569049No Hit
CGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGCCGGGCCCCCC362600.11150064929294258No Hit
GCCGCCCCGGCGGTGAACGGGGAGGAGGCGGGAACCGAAGAAGCGGGGCG362380.11143299859563303No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC360590.11088256792206885No Hit
ACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTC360550.11087026779528529No Hit
GGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGT358850.11034751240698415No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT355260.1092435760281599No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC354930.10914209998219558No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA347690.10691577703437179No Hit
GTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGC346470.10654062316747331No Hit
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC346440.10653139807238562No Hit
CCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGA339410.10436965079017552No Hit
CCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGA339370.10435735066339197No Hit
CCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACG336000.10332106498187729No Hit
CTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGG332850.10235242999767222No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA330940.10176509894375738No Hit
GCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCGCCG325350.1000461562257553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)