FastQCFastQC Report
Mon 18 Sep 2023
EGAF00002273210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002273210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56854931
Sequences flagged as poor quality0
Sequence length16-125
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATGCGCCCGGCGG6487531.1410672541313962No Hit
CCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCG6366391.1197603951009982No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC5845701.0281781891530217No Hit
CCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATGCGCCCGGCGGCG3919780.6894353631349934No Hit
CCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGC3059980.5382083745735264No Hit
CCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATGCGCCCGGCGGCGG2726170.47949578902839574No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCCCCTCG2699720.47484359799856235No Hit
CCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCGCAC2541720.4470535721870808No Hit
CCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATGCGC2418980.42546529517378184No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCTCCGGGGGCGGGGAGCG2118190.3725604732507722No Hit
CCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG1999550.35169332981865725No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC1809160.31820634871582204No Hit
CCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCG1621020.28511511165144143No Hit
GCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCG1615790.28419522661983354No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1607190.28268260496174025No Hit
GGGGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGC1548730.2724002954114921No Hit
GCCGGTCGCGGCGCACCGCCGCGGTGGAAATGCGCCCGGCGGCGGCCGGT1460610.2569012000032152No Hit
GGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCC1375390.2419121746889465No Hit
CCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG1330410.23400081164463993No Hit
GCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGC1288130.2265643414464789No Hit
GGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGC1265600.22260162447475312No Hit
GTCGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCG1251430.22010931646368545No Hit
CCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCG1236560.21749388808509854No Hit
GGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGA1133230.19931956297686826No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA1120530.19708580773759096No Hit
CTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCG1094440.19249693575391025No Hit
GGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTC1064860.18729422079502656No Hit
GTCGGATTCCCCTGGTCCGCACCAGTTCTAAGTCGGCTGCTAGGCGCCGG1014590.1784524195447533No Hit
GACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGC1008250.17733730078750778No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG1005980.17693803902426686No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCC969020.17043728361925195No Hit
GGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAA967430.170157624498744No Hit
CGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGG929790.16353726645099614No Hit
GGGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCG921080.1620052973065784No Hit
GGTCGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCC916640.16122436240402788No Hit
CCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCGCA904370.15906623824765526No Hit
GGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCGATGCTTT903070.15883758613654814No Hit
GCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAA902490.1587355721177465No Hit
GCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATGCGCCCGG900050.15830640969382234No Hit
CTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCC883670.15542539309387254No Hit
CCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATGCGCC880240.15482210329302834No Hit
CCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGC870430.15309665928536612No Hit
CAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATGCGCCC845520.14871533306407494No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC832550.14643408854018308No Hit
GCCCCGGGGGCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCG815770.14348271744450802No Hit
CGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC805460.1416693303171892No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG800550.14080572888216153No Hit
GCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCCCCT769510.13534622001388058No Hit
GTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGG756320.13302628051733983No Hit
CCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCTCCGGGGGCGGGGAG744240.13090157474643668No Hit
CACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCT743440.13076086575498613No Hit
GACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTC736980.12962464064902304No Hit
CTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATG735310.12933091062937002No Hit
GGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCA725820.12766175021828802No Hit
CGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCCCC705180.12403145823886409No Hit
CTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTG703500.12373596935681797No Hit
GGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGG699200.12297965852777132No Hit
CCGGTCGCGGCGCACCGCCGCGGTGGAAATGCGCCCGGCGGCGGCCGGTC697110.12261205628760678No Hit
CCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGG691460.12161829903548735No Hit
GCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCG689080.12119968978592201No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA687990.12100797378507062No Hit
GGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCAT685570.12058232908593275No Hit
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCC683870.12028332247910037No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGC664250.11683243446377589No Hit
CGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGT659240.11595124440481688No Hit
GCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAA653420.11492758649201422No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG651580.11460395581167797No Hit
CGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATGCGCCCGGCGGCGGC646210.11365944670656623No Hit
GGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGA643430.1131704829612756No Hit
GGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCG634870.11166489675275483No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT620230.10908992220920997No Hit
CCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCC618510.1087873978775913No Hit
CGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGG615370.10823511508614794No Hit
CACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCC602940.1060488491314852No Hit
GGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTT599070.10536816938534321No Hit
GTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCGCGGCC592960.10429350446313972No Hit
CGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTC579740.10196828837941954No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC577780.10162355135036573No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCT571860.10058230481363173No Hit
GTCGCGGCGCACCGCCGCGGTGGAAATGCGCCCGGCGGCGGCCGGTCGCC571250.1004750142076507No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)