FastQCFastQC Report
Sun 14 May 2023
EGAF00002273215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002273215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49282973
Sequences flagged as poor quality0
Sequence length16-125
%GC70

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGAT4279960.8684459843767948No Hit
CGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTCCCGCCCCCCGTTCC3227510.6548935268170611No Hit
CTTTCCCGCCCCCCGTTCCTCCCGACCCCTCCACCCGCCCTCCCTTCCCC2915650.5916140651660767No Hit
GTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTCCCGCCCCCCGTTCCTCC2327890.4723517795892711No Hit
CGTTCCTCCCGACCCCTCCACCCGCCCTCCCTTCCCCCGCCGCCCCTCCT2242330.45499081396733104No Hit
CCCGACCCCTCCACCCGCCCTCCCTTCCCCCGCCGCCCCTCCTCCTCCTC2048150.41558978189079626No Hit
GGTAAACGGGTGGGGTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGG2045470.4150459835286317No Hit
CCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTCCCGCCCC1963550.3984236097120196No Hit
CCTCCACCCGCCCTCCCTTCCCCCGCCGCCCCTCCTCCTCCTCCCCGGAG1930550.391727585103277No Hit
CCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGG1915300.3886332100946913No Hit
CCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTCCCGCCC1885840.38265548630761376No Hit
GGTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGT1868930.37922428097022476No Hit
ATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTC1727390.3505044226938176No Hit
CAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTCCCG1673620.3395939607782996No Hit
GTTCAAGAGGGCGTGAAACCGTTAAGAGGTAAACGGGTGGGGTCCGCGCA1574830.3195484980177637No Hit
GAAACCGTTAAGAGGTAAACGGGTGGGGTCCGCGCAGTCCGCCCGGAGGA1561280.3167990697314466No Hit
CCCGCCCCCCGTTCCTCCCGACCCCTCCACCCGCCCTCCCTTCCCCCGCC1497970.3039528479744921No Hit
CCGGCCGTGTCGGCGGCCCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCG1356680.27528371715724215No Hit
CCCCCACGCCCGGGGCACCCCCCTCGCGGCCCTCCCCCGCCCCACCCCGC1338460.27158669993386964No Hit
CGGGTGGGGTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTC1205170.24454084780964816No Hit
AACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTCCCGC1187870.241030507635974No Hit
GTAAACGGGTGGGGTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGC1154530.23426549368277763No Hit
CGTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCC1056040.2142809038732302No Hit
TTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTCC1050040.21306344485345882No Hit
GTTCCTCCCGACCCCTCCACCCGCCCTCCCTTCCCCCGCCGCCCCTCCTC1032030.20940903869577834No Hit
CCGTTAAGAGGTAAACGGGTGGGGTCCGCGCAGTCCGCCCGGAGGATTCA1029910.2089788698421258No Hit
TTCCCGCCCCCCGTTCCTCCCGACCCCTCCACCCGCCCTCCCTTCCCCCG1010900.20512155384781677No Hit
CCCGTTCCTCCCGACCCCTCCACCCGCCCTCCCTTCCCCCGCCGCCCCTC947610.19227939028759486No Hit
CCGCCCCCCGTTCCTCCCGACCCCTCCACCCGCCCTCCCTTCCCCCGCCG945810.19191415258166344No Hit
CCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCT939020.1905363947909555No Hit
GGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCC933830.18948329273885325No Hit
GGAAAGTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCG928370.18837540503086125No Hit
GTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAG918520.18637674314006988No Hit
GAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATC913740.18540683412098535No Hit
CGGCCGTGTCGGCGGCCCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGA891150.18082310091154605No Hit
GCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTCCCGCCCCCCGTTC864490.1754135246670285No Hit
CCCGCCCTCCCTTCCCCCGCCGCCCCTCCTCCTCCTCCCCGGAGGGGGCG860720.17464855458293882No Hit
AACCGTTAAGAGGTAAACGGGTGGGGTCCGCGCAGTCCGCCCGGAGGATT856890.1738714099086514No Hit
CGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTC842910.17103473039258407No Hit
CCACGCCCGGGGCACCCCCCTCGCGGCCCTCCCCCGCCCCACCCCGCGCG839930.17043005907943096No Hit
GGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTC820840.16655651029819163No Hit
CCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGC810180.16439349143973112No Hit
CCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGA793020.16091155864318493No Hit
GGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTT783870.15905493363803355No Hit
GGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTC703080.14266184793681178No Hit
GGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTCCCGCCCCCCGTTCCTC693850.1407889901447301No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCC688330.13966892784654042No Hit
GGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCG680470.1380740565306399No Hit
TCCCGCCCCCCGTTCCTCCCGACCCCTCCACCCGCCCTCCCTTCCCCCGC675930.13715284587234622No Hit
GTCGGCGGCCCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCC671960.1363472938209308No Hit
CACGCCCGGGGCACCCCCCTCGCGGCCCTCCCCCGCCCCACCCCGCGCGC668830.1357121860322834No Hit
CCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCG666830.13530636635902626No Hit
GTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTG663120.1345535708651343No Hit
CGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTG663120.1345535708651343No Hit
CCGTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGC660940.134111227421284No Hit
ATTGGCTCTAAGGGCTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGG660880.1340990528310863No Hit
CGGCGGCCCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCCAC657650.13344365405877603No Hit
GGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCCACCCGCCCTCC649440.13177776430005553No Hit
GCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGC640130.12988867372104357No Hit
CCCCCCTCCCGGCGCCCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTCT637750.1294057483098676No Hit
CTTCCCCCGCCGCCCCTCCTCCTCCTCCCCGGAGGGGGCGGGCTCCGGCG628040.12743549379620422No Hit
CCGACCCCTCCACCCGCCCTCCCTTCCCCCGCCGCCCCTCCTCCTCCTCC626100.1270418487131448No Hit
CAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAGAGAGTTCA606840.12313380525967862No Hit
CGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCCACCCGCCCTCCCT606000.12296336099691065No Hit
GGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTCCCGCCCCCCGTTCCT602190.12219027451935581No Hit
CTCCACCCGCCCTCCCTTCCCCCGCCGCCCCTCCTCCTCCTCCCCGGAGG602080.12216795443732666No Hit
GCCGTGTCGGCGGCCCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACC598410.12142327533689982No Hit
CCGTTCCTCCCGACCCCTCCACCCGCCCTCCCTTCCCCCGCCGCCCCTCC591580.12003740115272671No Hit
GATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCCACCCGCCCTCCCTTC590080.11973303639778386No Hit
GTTAAGAGGTAAACGGGTGGGGTCCGCGCAGTCCGCCCGGAGGATTCAAC586840.11907560852710733No Hit
GGGAAAGTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACC584650.11863123598489078No Hit
GCTGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT576460.11696940442290282No Hit
ACCGTTAAGAGGTAAACGGGTGGGGTCCGCGCAGTCCGCCCGGAGGATTC572580.11618211425678399No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA568410.11533598023804287No Hit
CTTCGGGATAAGGATTGGCTCTAAGGGCTGGGTCGGTCGGGCTGGGGCGC567990.11525075810665886No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG559620.11355240277407777No Hit
CCCCGTTCCTCCCGACCCCTCCACCCGCCCTCCCTTCCCCCGCCGCCCCT556600.11293961506745952No Hit
CGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCC547790.11115197940676183No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG543070.11019424497787501No Hit
GGCGGCCCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCCACC542740.1101272847317876No Hit
GTTACAGCCCCCCCGGCAGCAGCACTCGCCGAATCCCGGGGCCGAGGGAG536000.10875967243291106No Hit
GATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTT534820.10852023882568936No Hit
CCCGCCGCCCCTCCTCCTCCTCCCCGGAGGGGGCGGGCTCCGGCGGGTGC531220.10778976341382651No Hit
GAGGGCGTGAAACCGTTAAGAGGTAAACGGGTGGGGTCCGCGCAGTCCGC520720.10565921012922659No Hit
AGGTAAACGGGTGGGGTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCG519650.105442096604034No Hit
GGGAAGTCGGCAAGCCGGATCCGTAACTTCGGGATAAGGATTGGCTCTAA518070.10512149906216088No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT510610.10360779168091178No Hit
GTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG510060.10349619127076608No Hit
GGGTGGGGTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCC496500.10074473388608272No Hit
CCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAG495130.10046674740990161No Hit
GGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCT493890.10021513921248217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)