FastQCFastQC Report
Thu 16 Mar 2023
EGAF00002273219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002273219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115345393
Sequences flagged as poor quality0
Sequence length16-125
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA4827340.41851173024309696No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA3077400.2667986921679655No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG2910140.2522978962844229No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCA2811480.24374445540273987No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT2795070.24232177179369443No Hit
CTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGG2530080.21934816243592842No Hit
GGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGCCGGG2489910.21586557861049552No Hit
GACGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGCCGGGCCCC2465050.21371031264334933No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCA2411630.20907900500195964No Hit
CGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGG2301310.19951468716223456No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA2162600.187489065991565No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC2155070.1868362440795533No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA1981570.17179446430079787No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG1976870.17138699245664712No Hit
GTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCAC1920730.16651987132247234No Hit
CGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTA1869360.16206629076204196No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA1855350.16085167788192462No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCT1844800.1599370336360118No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA1835730.15915069967293796No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACC1797860.15586751696272774No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG1787290.1549511387940739No Hit
TCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTC1769650.15342181893645288No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTA1764580.1529822695215924No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT1759540.15254532099084356No Hit
CGGACGGCGCCGGACGCGCACGCCCCGCCGGGCCCCCCGCACGCACGCGC1743180.15112697218865082No Hit
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG1742920.15110443119301697No Hit
CCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACG1694060.14686845793659048No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC1690990.14660230079583675No Hit
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCT1658250.14376386926871018No Hit
ACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTC1644760.14259433837986055No Hit
CGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGCTAGT1634700.1417221752411039No Hit
CCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGAC1617830.14025961140901397No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC1612450.13979318619166697No Hit
GTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGG1606680.13929294948086918No Hit
CAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAAC1597480.1384953450199784No Hit
GCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAA1589480.13780177592355164No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1579120.13690360394367898No Hit
CGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA1577330.13674841785835345No Hit
GTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCGGGCCC1550390.13441282392613635No Hit
TGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGT1491620.12931769195151124No Hit
GAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCA1483490.12861285235726752No Hit
CCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAA1471980.1276149798197835No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGG1456380.12626252008175132No Hit
CAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGT1455800.1262122363222604No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT1449990.12570853176598046No Hit
TGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTC1435560.12445750650830069No Hit
GTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCCCGTTC1400460.12141447209772825No Hit
AGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGG1387870.12032296773222663No Hit
GGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTG1373070.11903986490383713No Hit
GGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCA1371970.11894449915307843No Hit
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC1363630.11822145337005353No Hit
ACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGAC1360770.11797350241808098No Hit
CACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTT1330560.11535441211769941No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA1327570.11509519066790991No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1314310.11394559989058253No Hit
GAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGC1313330.11386063767627025No Hit
CTCGCGGGGACACCGGGGGGGCGCCGGGGGCCTCCCACTTATTCTACACC1301450.11283068756807653No Hit
CATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCAC1299450.11265729529396983No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC1295100.11228016709778778No Hit
ACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGC1294150.11219780576758709No Hit
CACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCAT1278350.11082800680214423No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC1274680.11050983197915847No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA1267690.10990382598115556No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC1261520.10936891081553643No Hit
CCCGCACGCACGCGCGCGCGCGCGCGCGGACAAACCCTTGTGTCGAGGGC1256500.10893369620752864No Hit
CAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCT1250260.10839271231231576No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGA1246480.10806500091425411No Hit
CATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTA1242480.10771821636604073No Hit
ACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATG1235220.10708880241103345No Hit
TGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGC1227700.10643684746039228No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT1214730.10531239856281038No Hit
GGGAGGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGC1184520.10269330826242884No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG1182990.10256066317273721No Hit
GGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTC1164540.10096111944410298No Hit
CCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAA1158520.10043920869904184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)