FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002287070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002287070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894159
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGTCAATCTCGTAT148311.6586535504311872TruSeq Adapter, Index 18 (97% over 38bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18140.20287219610829843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)