FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002287094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002287094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1389163
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTTCACAATCTCGTAT590744.2524887288244795TruSeq Adapter, Index 8 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTTCACAACCTCGTAT22680.16326377826072247TruSeq Adapter, Index 8 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCTTCACAATCTCGTATGCC19670.1415960546026636TruSeq Adapter, Index 8 (97% over 34bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19650.1414520830168958No Hit
GAAGAGCACACGTCTGAACTCCAGTCACTCTTCACAATCTCGTATGCCGT15760.11344960958505229TruSeq Adapter, Index 8 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)