FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002287106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002287106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237332
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGACAACATCTCGTAT148031.1963644357375385TruSeq Adapter, Index 16 (97% over 38bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18960.1532329237423747No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATAT12440.10053890144278171No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)