FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002287246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002287246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127428
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAACAAATCTCGTAT410433.6404098532234435TruSeq Adapter, Index 10 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28600.2536747357702665No Hit
GAAGAGCACACGTCTGAACTCCAGTCACTGGAACAAATCTCGTATGCCGT25540.22653331299204915TruSeq Adapter, Index 10 (96% over 31bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGGAACAAATCTCGTATGCC25530.2264446155319896TruSeq Adapter, Index 10 (96% over 33bp)
ATATATATATATATATATATATATATATATATATATATATATATATATAT19700.17473399631728145No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAACAAACCTCGTAT15510.13756976055233683TruSeq Adapter, Index 10 (97% over 36bp)
TATATATATATATATATATATATATATATATATATATATATATATATATA14560.12914350184668114No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)